Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OG389_RS32230 Genome accession   NZ_CP107924
Coordinates   7147111..7147740 (-) Length   209 a.a.
NCBI ID   WP_328302208.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_00435     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7142111..7152740
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OG389_RS32205 (OG389_32205) - 7142770..7143474 (+) 705 WP_328302200.1 M24 family metallopeptidase -
  OG389_RS32210 (OG389_32210) - 7143578..7143994 (-) 417 WP_328302202.1 TOBE domain-containing protein -
  OG389_RS32215 (OG389_32215) - 7144277..7144681 (+) 405 WP_328302204.1 helix-turn-helix transcriptional regulator -
  OG389_RS32220 (OG389_32220) - 7144853..7145020 (+) 168 Protein_6348 substrate-binding domain-containing protein -
  OG389_RS32225 (OG389_32225) - 7145152..7147074 (+) 1923 WP_328302206.1 ABC transporter permease -
  OG389_RS32230 (OG389_32230) pilT 7147111..7147740 (-) 630 WP_328302208.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  OG389_RS32235 (OG389_32235) - 7147983..7148816 (-) 834 WP_328302211.1 class II glutamine amidotransferase -
  OG389_RS32240 (OG389_32240) - 7149172..7149786 (+) 615 WP_328302213.1 DUF308 domain-containing protein -
  OG389_RS32245 (OG389_32245) - 7150027..7150410 (-) 384 WP_328302214.1 hypothetical protein -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 22912.22 Da        Isoelectric Point: 11.7769

>NTDB_id=649503 OG389_RS32230 WP_328302208.1 7147111..7147740(-) (pilT) [Streptomyces sp. NBC_00435]
MHILTIEDPIEYLHHHKRSAVNQREVGTDTDSFPAALRAALREDPDVLLLGEMRDPESIAAALTIAETGHLVFATLHTND
TSQTLDRIVYVFPAEQQPQIRLQLAHTLVGILNQTLIPRIGGGRVAAFEVLAGTSAIRNLRGGRAAHRLPGGHPAALRPG
RPHLTGRQGRGPHAVRLGAARAPQQPRVRVHHAIEGHRMRREVPPGRRP

Nucleotide


Download         Length: 630 bp        

>NTDB_id=649503 OG389_RS32230 WP_328302208.1 7147111..7147740(-) (pilT) [Streptomyces sp. NBC_00435]
GTGCACATCCTCACCATTGAGGACCCGATCGAGTACCTGCACCACCACAAGCGCTCGGCGGTCAACCAGCGCGAGGTCGG
CACCGACACCGACTCGTTCCCCGCCGCGCTGCGCGCGGCCCTGCGCGAGGATCCCGACGTCCTGCTCCTCGGCGAGATGC
GCGACCCGGAGAGCATCGCCGCGGCCCTGACCATCGCCGAGACCGGGCACCTGGTGTTCGCCACCCTGCACACCAACGAC
ACGTCGCAGACACTGGACCGCATCGTCTACGTGTTCCCGGCCGAGCAACAGCCACAGATCCGGCTGCAATTGGCGCACAC
CCTGGTCGGCATCCTCAACCAGACGCTCATCCCCCGGATCGGCGGGGGCCGGGTGGCCGCCTTCGAGGTGCTGGCAGGCA
CGTCGGCGATCCGCAACCTACGAGGAGGCCGTGCTGCACACCGCCTACCCGGAGGACATCCAGCGGCCCTACGTCCCGGT
CGGCCGCACCTGACCGGGCGGCAAGGCCGAGGACCACACGCCGTACGACTGGGCGCCGCCCGGGCACCTCAGCAACCGCG
CGTTCGAGTTCATCACGCGATCGAGGGCCACCGCATGCGCAGAGAGGTCCCACCCGGACGCCGCCCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(2-91)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.411

67.464

0.421
  pilT Pseudomonas aeruginosa PAK

61.871

66.507

0.411
  pilT Pseudomonas stutzeri DSM 10701

60.432

66.507

0.402
  pilT Acinetobacter baylyi ADP1

57.143

70.335

0.402
  pilT Legionella pneumophila strain Lp02

50

80.383

0.402
  pilT Legionella pneumophila strain ERS1305867

50

80.383

0.402
  pilT Neisseria meningitidis 8013

57.143

66.986

0.383
  pilT Acinetobacter nosocomialis M2

57.554

66.507

0.383
  pilT Acinetobacter baumannii D1279779

57.554

66.507

0.383
  pilT Acinetobacter baumannii strain A118

57.554

66.507

0.383
  pilT Vibrio cholerae strain A1552

56.835

66.507

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.835

66.507

0.378
  pilT Neisseria gonorrhoeae MS11

56.429

66.986

0.378