Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   L0B52_RS05070 Genome accession   NZ_CP090975
Coordinates   1088412..1088873 (-) Length   153 a.a.
NCBI ID   WP_311195327.1    Uniprot ID   -
Organism   Suttonella sp. R2A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1083412..1093873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0B52_RS05050 (L0B52_05050) argF 1085031..1086032 (-) 1002 WP_235063661.1 ornithine carbamoyltransferase -
  L0B52_RS05055 (L0B52_05055) - 1086115..1087728 (-) 1614 WP_235063662.1 PglL family O-oligosaccharyltransferase -
  L0B52_RS05060 (L0B52_05060) pilA/pilA1 1087902..1088336 (-) 435 WP_409202288.1 pilin Machinery gene
  L0B52_RS05070 (L0B52_05070) pilA/pilA1 1088412..1088873 (-) 462 WP_311195327.1 pilin Machinery gene
  L0B52_RS05080 (L0B52_05080) - 1089098..1090351 (+) 1254 WP_235063663.1 sodium:proton antiporter -
  L0B52_RS05085 (L0B52_05085) gshB 1091155..1092102 (-) 948 WP_235063664.1 glutathione synthase -
  L0B52_RS05090 (L0B52_05090) gshA 1092182..1093696 (+) 1515 WP_235063665.1 glutamate--cysteine ligase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15721.87 Da        Isoelectric Point: 4.2985

>NTDB_id=646837 L0B52_RS05070 WP_311195327.1 1088412..1088873(-) (pilA/pilA1) [Suttonella sp. R2A3]
MKKQMQKGFTLIELMIVIAIIGILAAIALPAYQDYIARAQASEAMKATTGLQSDIGVFLADQGNFPTASDLVSGQPIFDA
AAELEGKYFGASGVTVAATTGMITVTFDDGANSGNTVTLTPNQNAAGGQIASWTCAPGTTNGIDAKRLPSSCQ

Nucleotide


Download         Length: 462 bp        

>NTDB_id=646837 L0B52_RS05070 WP_311195327.1 1088412..1088873(-) (pilA/pilA1) [Suttonella sp. R2A3]
ATGAAAAAACAAATGCAAAAGGGTTTTACCCTAATCGAACTCATGATTGTGATCGCGATCATTGGTATTTTAGCAGCCAT
CGCGCTTCCAGCGTATCAAGACTACATTGCTCGCGCACAAGCATCTGAAGCGATGAAAGCAACCACCGGTTTGCAATCTG
ATATTGGTGTATTTTTAGCGGATCAAGGTAACTTCCCAACAGCATCAGATCTTGTTTCAGGTCAACCTATTTTTGATGCT
GCTGCTGAATTAGAGGGTAAATATTTCGGTGCTAGCGGTGTAACAGTAGCAGCGACTACTGGGATGATTACAGTTACCTT
TGATGATGGTGCAAATAGTGGTAATACTGTGACATTGACACCAAATCAGAACGCTGCTGGTGGACAAATTGCTAGTTGGA
CGTGTGCTCCTGGCACGACAAATGGTATCGATGCAAAACGCTTACCATCAAGCTGCCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)

Pilin (bacterial filament) (Pilin)

(37-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

66.013

100

0.66
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.968

100

0.464
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.741

100

0.431
  comP Acinetobacter baylyi ADP1

40.385

100

0.412
  pilA2 Legionella pneumophila str. Paris

40.132

99.346

0.399
  pilE Neisseria gonorrhoeae MS11

36.747

100

0.399
  pilE Neisseria gonorrhoeae strain FA1090

36.364

100

0.392
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.732

0.386
  pilA Ralstonia pseudosolanacearum GMI1000

34.302

100

0.386
  pilA Acinetobacter baumannii strain A118

36.076

100

0.373
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.013

100

0.373
  pilA Pseudomonas aeruginosa PAK

35.897

100

0.366