Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OHL99_RS02345 Genome accession   NZ_CP107270
Coordinates   456863..457993 (+) Length   376 a.a.
NCBI ID   WP_003704392.1    Uniprot ID   Q5F9P7
Organism   Neisseria gonorrhoeae strain 1081168     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 451863..462993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHL99_RS02320 (OHL99_02320) - 452309..452458 (+) 150 WP_003687747.1 hypothetical protein -
  OHL99_RS02325 (OHL99_02325) - 452682..452789 (+) 108 Protein_447 IS5/IS1182 family transposase -
  OHL99_RS02330 (OHL99_02330) lepB 452894..453913 (-) 1020 WP_003706641.1 signal peptidase I -
  OHL99_RS02335 (OHL99_02335) lepA 454059..455852 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  OHL99_RS02340 (OHL99_02340) - 455994..456695 (-) 702 WP_003692750.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  OHL99_RS02345 (OHL99_02345) pilU 456863..457993 (+) 1131 WP_003704392.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OHL99_RS02350 (OHL99_02350) - 458027..459004 (+) 978 WP_003687755.1 DNA polymerase III subunit delta' -
  OHL99_RS02355 (OHL99_02355) - 459008..459358 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  OHL99_RS02360 (OHL99_02360) - 459363..460142 (+) 780 WP_003690796.1 TatD family hydrolase -
  OHL99_RS02365 (OHL99_02365) - 460168..460383 (+) 216 WP_003690799.1 DUF333 domain-containing protein -
  OHL99_RS02370 (OHL99_02370) grxD 460484..460795 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  OHL99_RS02375 (OHL99_02375) upp 460901..461527 (+) 627 WP_003690802.1 uracil phosphoribosyltransferase -
  OHL99_RS02380 (OHL99_02380) - 461550..461870 (+) 321 WP_003690805.1 DUF2322 family protein -
  OHL99_RS02385 (OHL99_02385) - 462039..462680 (+) 642 Protein_459 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42215.32 Da        Isoelectric Point: 6.2891

>NTDB_id=642943 OHL99_RS02345 WP_003704392.1 456863..457993(+) (pilU) [Neisseria gonorrhoeae strain 1081168]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=642943 OHL99_RS02345 WP_003704392.1 456863..457993(+) (pilU) [Neisseria gonorrhoeae strain 1081168]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(87-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F9P7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.659

93.617

0.559
  pilU Acinetobacter baylyi ADP1

57.726

91.223

0.527
  pilU Vibrio cholerae strain A1552

54.366

94.415

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.343

93.883

0.407
  pilT Legionella pneumophila strain Lp02

43.827

86.17

0.378
  pilT Legionella pneumophila strain ERS1305867

43.827

86.17

0.378
  pilT Acinetobacter baylyi ADP1

42.727

87.766

0.375
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.896

89.096

0.364
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362