Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NDN54_RS02115 Genome accession   NZ_CP107266
Coordinates   412579..413709 (+) Length   376 a.a.
NCBI ID   WP_322740572.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 9112     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407579..418709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NDN54_RS02090 (NDN54_02090) - 408025..408174 (+) 150 WP_003687747.1 hypothetical protein -
  NDN54_RS02095 (NDN54_02095) - 408398..408505 (+) 108 Protein_407 IS5/IS1182 family transposase -
  NDN54_RS02100 (NDN54_02100) lepB 408610..409629 (-) 1020 WP_003706641.1 signal peptidase I -
  NDN54_RS02105 (NDN54_02105) lepA 409775..411568 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  NDN54_RS02110 (NDN54_02110) - 411710..412411 (-) 702 WP_003687752.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  NDN54_RS02115 (NDN54_02115) pilU 412579..413709 (+) 1131 WP_322740572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NDN54_RS02120 (NDN54_02120) - 413743..414720 (+) 978 WP_003687755.1 DNA polymerase III subunit delta' -
  NDN54_RS02125 (NDN54_02125) - 414724..415074 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  NDN54_RS02130 (NDN54_02130) - 415079..415858 (+) 780 WP_003694907.1 TatD family hydrolase -
  NDN54_RS02135 (NDN54_02135) - 415884..416099 (+) 216 WP_003690799.1 DUF333 domain-containing protein -
  NDN54_RS02140 (NDN54_02140) grxD 416200..416511 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  NDN54_RS02145 (NDN54_02145) upp 416617..417243 (+) 627 WP_003690802.1 uracil phosphoribosyltransferase -
  NDN54_RS02150 (NDN54_02150) - 417266..417586 (+) 321 WP_003690805.1 DUF2322 family protein -
  NDN54_RS02155 (NDN54_02155) - 417755..418396 (+) 642 Protein_419 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42203.26 Da        Isoelectric Point: 6.2891

>NTDB_id=642872 NDN54_RS02115 WP_322740572.1 412579..413709(+) (pilU) [Neisseria gonorrhoeae strain 9112]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRTINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=642872 NDN54_RS02115 WP_322740572.1 412579..413709(+) (pilU) [Neisseria gonorrhoeae strain 9112]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCACCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTAC
TACTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(87-258)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.085

94.415

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.059

93.883

0.404
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Acinetobacter baylyi ADP1

42.424

87.766

0.372
  pilT Pseudomonas aeruginosa PAK

42.378

87.234

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362
  pilT Pseudomonas stutzeri DSM 10701

41.463

87.234

0.362