Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LRR79_RS02365 Genome accession   NZ_CP088953
Coordinates   584900..586048 (+) Length   382 a.a.
NCBI ID   WP_231758819.1    Uniprot ID   -
Organism   Microbulbifer elongatus strain DSM 6810     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579900..591048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRR79_RS02345 - 581439..582044 (-) 606 WP_231758816.1 YggT family protein -
  LRR79_RS02350 proC 582069..582896 (-) 828 WP_231758817.1 pyrroline-5-carboxylate reductase -
  LRR79_RS02355 - 582920..583618 (-) 699 WP_231758818.1 YggS family pyridoxal phosphate-dependent enzyme -
  LRR79_RS02360 pilT 583730..584764 (+) 1035 WP_010133748.1 type IV pilus twitching motility protein PilT Machinery gene
  LRR79_RS02365 pilU 584900..586048 (+) 1149 WP_231758819.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRR79_RS02370 - 586073..586690 (+) 618 WP_231758820.1 LysE family translocator -
  LRR79_RS02375 - 586717..587475 (-) 759 WP_231758821.1 LytTR family DNA-binding domain-containing protein -
  LRR79_RS02380 - 587472..588758 (-) 1287 WP_231758822.1 sensor histidine kinase -
  LRR79_RS02385 argH 588871..590292 (+) 1422 WP_231758823.1 argininosuccinate lyase -
  LRR79_RS02390 cyaY 590383..590715 (-) 333 WP_231758824.1 iron donor protein CyaY -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42562.87 Da        Isoelectric Point: 6.2372

>NTDB_id=634123 LRR79_RS02365 WP_231758819.1 584900..586048(+) (pilU) [Microbulbifer elongatus strain DSM 6810]
MEIERLLQLVTEKGASDLFITAGVPASIKLHGKIVPVTTSPLTPEKAREMVIGIMNDRQKKEFVENKELNFAISLRNVGR
FRVSAFFQRNLVGMVLRRIETKIPTIDGLGLPEQLKELAMTKRGLIIFVGATGTGKSTSLASMIGHRNENSKGHIISIED
PIEFIHQHRGCIVTQREVGLDTDSFETALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHQQLWMDLSLNLQAMVAQQLVPTPDGDGRRACLEIMINTPLMSDLIRKGEVHKMKELMKRSTEQGMQTFDQA
LYELYDRGEITYEDALAHADSANDLRLMIKLKSESDAEYLNSAAEGLSLASDTDTGSGPQIY

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=634123 LRR79_RS02365 WP_231758819.1 584900..586048(+) (pilU) [Microbulbifer elongatus strain DSM 6810]
ATGGAAATCGAACGACTCCTACAACTGGTCACCGAAAAAGGCGCGTCTGACCTGTTCATCACCGCCGGTGTACCCGCGTC
CATCAAACTGCACGGCAAGATCGTCCCGGTCACCACCAGCCCGCTGACCCCGGAAAAAGCCCGGGAAATGGTGATCGGCA
TCATGAACGACCGCCAGAAAAAAGAGTTCGTTGAAAACAAAGAACTCAATTTCGCCATCTCCCTGCGCAACGTCGGCCGC
TTCCGGGTATCCGCCTTCTTCCAGCGCAACCTGGTGGGCATGGTACTGCGTCGTATCGAGACCAAGATCCCCACCATCGA
CGGACTCGGCCTGCCGGAGCAGCTCAAAGAGCTGGCCATGACCAAGCGCGGCCTGATCATCTTTGTGGGTGCGACCGGTA
CCGGTAAATCCACCTCGTTGGCCTCCATGATCGGCCACCGCAACGAGAACTCGAAGGGTCACATCATCTCCATCGAGGAC
CCCATCGAATTTATCCACCAGCACCGCGGCTGCATCGTCACCCAGCGGGAAGTCGGCCTGGATACCGACTCCTTCGAGAC
CGCCCTGAAAAACACCCTGCGTCAGGCACCCGACGTGATCCTGATTGGTGAGGTGCGTTCCCGCGAAACCATGGACCACG
CCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTGTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCACTTCTTCCCGGCGGACCGTCATCAGCAACTGTGGATGGACCTGTCCCTGAACCTGCAGGCGATGGTCGCCCAGCA
GCTGGTCCCCACTCCGGACGGCGACGGCCGCCGCGCCTGTCTCGAGATCATGATCAACACCCCGCTGATGTCGGACCTGA
TCCGCAAGGGCGAGGTGCACAAAATGAAGGAGCTGATGAAACGCTCCACCGAGCAGGGTATGCAGACCTTCGACCAGGCC
CTGTACGAACTCTACGACCGCGGCGAAATCACCTACGAAGACGCCCTCGCCCACGCGGACTCCGCCAACGACCTGCGCCT
GATGATCAAGCTCAAGTCCGAATCCGACGCCGAGTACCTCAACAGCGCCGCCGAGGGACTGAGCCTCGCCAGCGACACCG
ATACCGGCAGCGGCCCGCAGATCTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.127

98.691

0.751
  pilU Acinetobacter baylyi ADP1

66.667

93.455

0.623
  pilU Vibrio cholerae strain A1552

55.056

93.194

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.627

97.644

0.416
  pilT Legionella pneumophila strain Lp02

42.687

87.696

0.374
  pilT Legionella pneumophila strain ERS1305867

42.687

87.696

0.374
  pilT Pseudomonas aeruginosa PAK

41.194

87.696

0.361
  pilT Acinetobacter nosocomialis M2

41.194

87.696

0.361
  pilT Acinetobacter baumannii D1279779

41.194

87.696

0.361
  pilT Acinetobacter baumannii strain A118

41.194

87.696

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

87.435

0.361
  pilT Vibrio cholerae strain A1552

41.317

87.435

0.361