Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   LOY49_RS22390 Genome accession   NZ_CP087167
Coordinates   5007258..5007680 (-) Length   140 a.a.
NCBI ID   WP_258603688.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5002258..5012680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY49_RS22375 (LOY49_22345) - 5003127..5006078 (+) 2952 WP_258603685.1 DUF748 domain-containing protein -
  LOY49_RS22380 (LOY49_22350) - 5006075..5006374 (+) 300 WP_024014339.1 DUF2845 domain-containing protein -
  LOY49_RS22385 (LOY49_22355) - 5006610..5006963 (-) 354 WP_016773155.1 BON domain-containing protein -
  LOY49_RS22390 (LOY49_22360) pilA/pilAI 5007258..5007680 (-) 423 WP_258603688.1 pilin Machinery gene
  LOY49_RS22395 (LOY49_22365) pilB 5007907..5009607 (+) 1701 WP_258603690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY49_RS22400 (LOY49_22370) pilC 5009610..5010827 (+) 1218 WP_258603692.1 type II secretion system F family protein Machinery gene
  LOY49_RS22405 (LOY49_22375) pilD 5010829..5011701 (+) 873 WP_258603693.1 A24 family peptidase Machinery gene
  LOY49_RS22410 (LOY49_22380) coaE 5011698..5012321 (+) 624 WP_258603694.1 dephospho-CoA kinase -
  LOY49_RS22415 (LOY49_22385) yacG 5012318..5012518 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14103.22 Da        Isoelectric Point: 8.1079

>NTDB_id=627126 LOY49_RS22390 WP_258603688.1 5007258..5007680(-) (pilA/pilAI) [Pseudomonas atacamensis strain B21-050]
MTKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDLINQGTNPTLAAVKGDNVTTTSNCA
LTASGTAADGAGSIVCTLANAPAPVLGKTITLSRTAAGGWSCGTTVAPDYSPKGCNATGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=627126 LOY49_RS22390 WP_258603688.1 5007258..5007680(-) (pilA/pilAI) [Pseudomonas atacamensis strain B21-050]
ATGACAAAACAACAAGGTTTCACATTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCGACTATCGCCCT
GCCTCAGTATTCGAAGTATCAGGCGCGGTCGAAGGTGACGGCGGGGTTGGCGGAGATCAGCGCGATGAAGGTGCCGTTTG
AAGACCTGATCAATCAGGGGACAAATCCAACCTTGGCGGCAGTGAAAGGCGATAACGTCACCACGACCTCAAATTGCGCT
CTGACTGCCTCAGGTACCGCTGCGGATGGAGCAGGATCGATTGTATGTACGCTGGCCAACGCGCCGGCACCTGTTTTAGG
GAAAACCATCACGCTGTCTCGTACGGCGGCCGGCGGTTGGAGTTGTGGCACTACGGTCGCTCCTGACTACTCGCCAAAAG
GCTGTAACGCTACTGGTACTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

49.635

97.857

0.486
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.909

100

0.45
  pilA Vibrio cholerae strain A1552

40.909

100

0.45
  pilA Vibrio cholerae C6706

40.909

100

0.45
  comP Acinetobacter baylyi ADP1

41.176

100

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA Pseudomonas aeruginosa PAK

39.073

100

0.421
  pilA2 Legionella pneumophila str. Paris

37.671

100

0.393
  pilA Vibrio parahaemolyticus RIMD 2210633

40.299

95.714

0.386
  pilA2 Legionella pneumophila strain ERS1305867

35.616

100

0.371