Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LOY49_RS22395 Genome accession   NZ_CP087167
Coordinates   5007907..5009607 (+) Length   566 a.a.
NCBI ID   WP_258603690.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-050     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5002907..5014607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY49_RS22375 (LOY49_22345) - 5003127..5006078 (+) 2952 WP_258603685.1 DUF748 domain-containing protein -
  LOY49_RS22380 (LOY49_22350) - 5006075..5006374 (+) 300 WP_024014339.1 DUF2845 domain-containing protein -
  LOY49_RS22385 (LOY49_22355) - 5006610..5006963 (-) 354 WP_016773155.1 BON domain-containing protein -
  LOY49_RS22390 (LOY49_22360) pilA/pilAI 5007258..5007680 (-) 423 WP_258603688.1 pilin Machinery gene
  LOY49_RS22395 (LOY49_22365) pilB 5007907..5009607 (+) 1701 WP_258603690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY49_RS22400 (LOY49_22370) pilC 5009610..5010827 (+) 1218 WP_258603692.1 type II secretion system F family protein Machinery gene
  LOY49_RS22405 (LOY49_22375) pilD 5010829..5011701 (+) 873 WP_258603693.1 A24 family peptidase Machinery gene
  LOY49_RS22410 (LOY49_22380) coaE 5011698..5012321 (+) 624 WP_258603694.1 dephospho-CoA kinase -
  LOY49_RS22415 (LOY49_22385) yacG 5012318..5012518 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  LOY49_RS22420 (LOY49_22390) - 5012526..5012741 (-) 216 WP_056790451.1 hypothetical protein -
  LOY49_RS22425 (LOY49_22395) - 5012807..5013496 (-) 690 WP_258603695.1 energy-coupling factor ABC transporter permease -
  LOY49_RS22430 (LOY49_22400) - 5013687..5014313 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62512.57 Da        Isoelectric Point: 6.1671

>NTDB_id=627127 LOY49_RS22395 WP_258603690.1 5007907..5009607(+) (pilB) [Pseudomonas atacamensis strain B21-050]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLSCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLAEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=627127 LOY49_RS22395 WP_258603690.1 5007907..5009607(+) (pilB) [Pseudomonas atacamensis strain B21-050]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAACTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGCGTCTCGCTGGTCAATTACCTGGTACACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATCGCTTCCGAACATTTCGGCATGGCCCTGCTCGATCTCAGCTGCCTCGACAAGGAGACTCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTTCGTCAGCACTGCGCCCTGCCCTTGTGGCGGCGCGGCAACAAACTGTTCGTGGGCCTTTCCGA
CCCGAGCAATCAGCAGGCGATCAGCGACATCCAGTTCAGCACCGGCCTGAGTACCGAAGCGATTCTGGTCGAGGACGACA
AACTCAGCGACGCCATCGACAAGTTCTTCGACAGCCACGCCTCTGGACTGGCGGAAATGGCCGATGTCGATCTCGACGGC
CTCGACGTCGAGTCCATTGATGACAACAAACAGGACGTGATCGGCGGCCTCGATGCCGACGACGCCCCGGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGTGGCTCTTCCGACCTGCACTTCGAGCCGTACGAAAAAAACTACCGGG
TGCGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGTATTGCCGCACGTTTG
AAGGTCATGGCCAGTCTGGATATTTCCGAACGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTTCGCGTCAACACCTTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAGATGGGCATCGATGCGCTGGGCTACGAGGCCGAACAAAAGGCCTTGTACCTGGCCGCGCTGAAACAGCCGCAAGGG
ATGATTTTGGTCACTGGCCCCACCGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAATATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAATCAGGTCAACGTCAATCCCCGCCAAG
GGCTGGACTTTGCCCAGGCGCTGCGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCAATCAAGGCTGCGCAGACCGGCCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCGGCGGA
AACCCTCACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATTGCCACCTCGGTCAGCCTGATCATAGCCCAGCGTC
TGGCGCGCAAATTGTGCGCGCACTGCAAGCGACCGCTGGAAATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAGAACACACCGGACCTGCAACGGTTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGATCTGCGCACTTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.624

99.117

0.502
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.209

100

0.495
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.462

94.17

0.362