Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LOY49_RS22400 Genome accession   NZ_CP087167
Coordinates   5009610..5010827 (+) Length   405 a.a.
NCBI ID   WP_258603692.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain B21-050     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5004610..5015827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY49_RS22380 (LOY49_22350) - 5006075..5006374 (+) 300 WP_024014339.1 DUF2845 domain-containing protein -
  LOY49_RS22385 (LOY49_22355) - 5006610..5006963 (-) 354 WP_016773155.1 BON domain-containing protein -
  LOY49_RS22390 (LOY49_22360) pilA/pilAI 5007258..5007680 (-) 423 WP_258603688.1 pilin Machinery gene
  LOY49_RS22395 (LOY49_22365) pilB 5007907..5009607 (+) 1701 WP_258603690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LOY49_RS22400 (LOY49_22370) pilC 5009610..5010827 (+) 1218 WP_258603692.1 type II secretion system F family protein Machinery gene
  LOY49_RS22405 (LOY49_22375) pilD 5010829..5011701 (+) 873 WP_258603693.1 A24 family peptidase Machinery gene
  LOY49_RS22410 (LOY49_22380) coaE 5011698..5012321 (+) 624 WP_258603694.1 dephospho-CoA kinase -
  LOY49_RS22415 (LOY49_22385) yacG 5012318..5012518 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  LOY49_RS22420 (LOY49_22390) - 5012526..5012741 (-) 216 WP_056790451.1 hypothetical protein -
  LOY49_RS22425 (LOY49_22395) - 5012807..5013496 (-) 690 WP_258603695.1 energy-coupling factor ABC transporter permease -
  LOY49_RS22430 (LOY49_22400) - 5013687..5014313 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  LOY49_RS22435 (LOY49_22405) - 5014310..5014837 (+) 528 WP_125925984.1 MOSC domain-containing protein -
  LOY49_RS22440 (LOY49_22410) - 5014889..5015062 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44082.76 Da        Isoelectric Point: 10.1214

>NTDB_id=627128 LOY49_RS22400 WP_258603692.1 5009610..5010827(+) (pilC) [Pseudomonas atacamensis strain B21-050]
MAVKAAKISVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSWGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=627128 LOY49_RS22400 WP_258603692.1 5009610..5010827(+) (pilC) [Pseudomonas atacamensis strain B21-050]
ATGGCGGTCAAGGCAGCGAAAATCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGGCAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAAGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGAGCTGGGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCAACGATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAGCTGGTCGACGA
GGTCAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCACGGCCCTGCGCAAGAAGCCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGACGCCGGCGAACAGTCTGGTGCCCTCGACACTTTGCTCGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCGGTGGTGACCGGCAT
TCTGCTGGTCAAGGTGGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCGATTCTGTTTGCACTGGTGGCAGCGTTTTTCGGCACTCGT
CACGCGCTGAAGACCTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCGCTGGTAGGCACCTTGAT
GTACAAGTCCGCCGTCGCCCGCTTTGCCCGCACCCTGTCGACCACTTTCGCCGCCGGGGTGCCATTGGTGGAAGCGCTGG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCTGCGCGTGCGGCAGGACGTCTCGACCGGCATG
CAGCTCAATTTTTCGATGCGCAGCACCGGTGTATTTCCGAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCACTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

58.578

100

0.59
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

39.401

99.012

0.39