Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   LMH63_RS02090 Genome accession   NZ_CP086615
Coordinates   446580..447002 (-) Length   140 a.a.
NCBI ID   WP_109679019.1    Uniprot ID   A0A2U2N0W4
Organism   Spiribacter halobius strain E85     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441580..452002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS02065 (LMH63_02065) - 441991..442704 (-) 714 WP_158280474.1 PEP-CTERM sorting domain-containing protein -
  LMH63_RS02070 (LMH63_02070) - 442938..443750 (-) 813 WP_373317891.1 ABC transporter permease -
  LMH63_RS02075 (LMH63_02075) - 443978..444538 (-) 561 WP_109680114.1 Uma2 family endonuclease -
  LMH63_RS02080 (LMH63_02080) - 444599..445555 (-) 957 WP_109680115.1 transposase -
  LMH63_RS02085 (LMH63_02085) - 445682..446173 (-) 492 WP_109680116.1 transposase -
  LMH63_RS02090 (LMH63_02090) pilA/pilA1 446580..447002 (-) 423 WP_109679019.1 pilin Machinery gene
  LMH63_RS02100 (LMH63_02100) pilR 447410..448744 (-) 1335 WP_109679018.1 sigma-54 dependent transcriptional regulator Regulator
  LMH63_RS02105 (LMH63_02105) - 448741..449769 (-) 1029 WP_109679017.1 PAS domain-containing sensor histidine kinase -
  LMH63_RS02110 (LMH63_02110) - 449797..450396 (-) 600 WP_109679016.1 hypothetical protein -
  LMH63_RS02115 (LMH63_02115) sucC 450805..451974 (+) 1170 WP_109679015.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14343.14 Da        Isoelectric Point: 3.8703

>NTDB_id=625707 LMH63_RS02090 WP_109679019.1 446580..447002(-) (pilA/pilA1) [Spiribacter halobius strain E85]
MKKQQGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQASEGLSATAGLRADISEQFALTDTIPAAAGDLNTNLNDIATA
QYISGVAYANGTITVTWDGATSSLAGDMTIAPVNNDPQQGWVCAAANGMEANHLPGGCAN

Nucleotide


Download         Length: 423 bp        

>NTDB_id=625707 LMH63_RS02090 WP_109679019.1 446580..447002(-) (pilA/pilA1) [Spiribacter halobius strain E85]
ATGAAGAAGCAGCAGGGCTTTACCCTTATCGAGCTGATGATCGTGGTGGCGATCATCGGTATTCTTGCGGCGATTGCGAT
TCCGGCTTATCAAGATTACACCGCACGTGCACAGGCTTCTGAAGGGCTGAGCGCGACGGCCGGTCTACGCGCAGATATCT
CGGAGCAGTTCGCACTGACCGACACAATTCCGGCCGCTGCAGGTGATCTGAACACTAACCTGAACGATATCGCGACTGCT
CAGTACATCAGCGGCGTTGCTTACGCCAATGGAACGATCACCGTCACGTGGGACGGCGCGACGTCATCGCTGGCGGGTGA
TATGACGATTGCTCCAGTTAACAACGATCCGCAGCAGGGCTGGGTGTGTGCCGCCGCTAACGGTATGGAGGCGAATCACC
TCCCCGGCGGGTGTGCTAACTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2N0W4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

48

100

0.514
  pilA Ralstonia pseudosolanacearum GMI1000

43.662

100

0.443
  comP Acinetobacter baylyi ADP1

39.865

100

0.421
  pilA2 Legionella pneumophila str. Paris

41.727

99.286

0.414
  pilA2 Legionella pneumophila strain ERS1305867

41.727

99.286

0.414
  pilA Acinetobacter baumannii strain A118

37.415

100

0.393
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.732

100

0.393
  pilE Neisseria gonorrhoeae MS11

34.395

100

0.386
  pilA Vibrio parahaemolyticus RIMD 2210633

42.857

90

0.386
  pilE Neisseria gonorrhoeae strain FA1090

33.544

100

0.379
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.957

98.571

0.364
  pilA Pseudomonas aeruginosa PAK

33.333

100

0.364