Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   LMH63_RS02100 Genome accession   NZ_CP086615
Coordinates   447410..448744 (-) Length   444 a.a.
NCBI ID   WP_109679018.1    Uniprot ID   A0A2U2N0M9
Organism   Spiribacter halobius strain E85     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 442410..453744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH63_RS02070 (LMH63_02070) - 442938..443750 (-) 813 WP_373317891.1 ABC transporter permease -
  LMH63_RS02075 (LMH63_02075) - 443978..444538 (-) 561 WP_109680114.1 Uma2 family endonuclease -
  LMH63_RS02080 (LMH63_02080) - 444599..445555 (-) 957 WP_109680115.1 transposase -
  LMH63_RS02085 (LMH63_02085) - 445682..446173 (-) 492 WP_109680116.1 transposase -
  LMH63_RS02090 (LMH63_02090) pilA/pilA1 446580..447002 (-) 423 WP_109679019.1 pilin Machinery gene
  LMH63_RS02100 (LMH63_02100) pilR 447410..448744 (-) 1335 WP_109679018.1 sigma-54 dependent transcriptional regulator Regulator
  LMH63_RS02105 (LMH63_02105) - 448741..449769 (-) 1029 WP_109679017.1 PAS domain-containing sensor histidine kinase -
  LMH63_RS02110 (LMH63_02110) - 449797..450396 (-) 600 WP_109679016.1 hypothetical protein -
  LMH63_RS02115 (LMH63_02115) sucC 450805..451974 (+) 1170 WP_109679015.1 ADP-forming succinate--CoA ligase subunit beta -
  LMH63_RS02120 (LMH63_02120) sucD 452010..452882 (+) 873 WP_109679014.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49852.92 Da        Isoelectric Point: 5.0574

>NTDB_id=625708 LMH63_RS02100 WP_109679018.1 447410..448744(-) (pilR) [Spiribacter halobius strain E85]
MSRPLALIVDDEADIRELAELTLARMDIEARSAANLESAYGLLERERFGLCLTDMRLPDGDGLDLVRHIQKHHPQLPVAM
ITAYGSVDTAIQALKAGAFDFVSKPVDLQILRRLVEHAFRLSEEPVDRRSRDTLLGESATMRRIRATIVKLARSQAPVYI
CGESGTGKELIARLIHDKGPRRDQPFVPVNCGAIPSELMESEFFGHKKGSFTGATSDKQGLFQAADGGTLFLDEVADLPQ
HMQVKLLRAIQEKAVRPVGASREQQVDVRILSATHKDLAELVESGDFRRDLFYRINVIQLRAPSLRERPDDIPMLVDHVL
ERLADGGQRPRLGETALDQLKRYPFPGNVRELENILERALTLCEGDEIQPEDLQLPETTGVELPEPPTAEPDDDFELEGY
LEGIERDLIERALEKTRYNKTAAAQLLGISFRALRYRLKKLNME

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=625708 LMH63_RS02100 WP_109679018.1 447410..448744(-) (pilR) [Spiribacter halobius strain E85]
ATGAGCCGACCCCTGGCCCTGATCGTCGACGACGAGGCCGATATCCGCGAGCTCGCCGAGCTCACGCTCGCCCGCATGGA
CATCGAGGCACGCAGCGCCGCCAACCTGGAGTCTGCCTACGGGCTGCTGGAGCGCGAGCGCTTCGGCCTGTGCCTGACGG
ACATGCGCCTGCCGGACGGCGACGGGCTGGATCTGGTGCGCCACATCCAGAAGCACCACCCGCAGCTCCCGGTGGCCATG
ATCACCGCCTACGGCAGCGTCGACACCGCCATTCAGGCACTCAAGGCCGGCGCCTTCGACTTCGTCAGCAAGCCGGTGGA
CCTGCAGATCCTGCGGCGGCTGGTGGAGCACGCCTTCCGGCTCTCCGAGGAGCCGGTGGACCGCCGCTCGCGGGACACCC
TGCTCGGCGAGTCGGCTACCATGCGCCGCATCCGCGCCACCATCGTCAAGCTCGCCCGCAGCCAGGCGCCGGTCTACATC
TGCGGCGAGTCCGGCACCGGCAAGGAGCTCATCGCCCGGCTCATCCACGACAAGGGCCCGCGGCGGGACCAGCCCTTCGT
GCCGGTGAACTGCGGCGCCATCCCCAGCGAGCTCATGGAGAGCGAGTTCTTCGGCCACAAGAAGGGCAGCTTCACCGGCG
CCACCAGCGACAAGCAGGGCCTCTTCCAGGCCGCCGACGGCGGCACGCTGTTCCTCGACGAGGTGGCCGACCTGCCCCAG
CACATGCAGGTGAAGCTCCTGCGCGCCATCCAGGAGAAGGCCGTGCGCCCGGTGGGCGCGAGCCGCGAGCAGCAGGTGGA
CGTGCGTATCCTCTCTGCCACCCACAAGGACCTCGCCGAGCTGGTGGAGTCCGGCGACTTCCGCCGCGACCTCTTCTACC
GCATCAACGTCATCCAGCTCCGCGCCCCCAGCCTGCGCGAGCGCCCCGACGACATCCCCATGCTCGTCGACCACGTCCTC
GAGCGCCTGGCGGACGGCGGCCAGCGCCCCCGCCTCGGCGAGACCGCCCTCGATCAGCTCAAGCGCTACCCGTTTCCGGG
CAACGTCCGCGAGCTCGAAAACATCCTCGAGCGCGCCCTCACCCTCTGCGAGGGCGACGAGATCCAGCCCGAGGACCTCC
AGCTCCCCGAGACCACCGGCGTCGAGCTCCCGGAGCCCCCCACCGCCGAGCCGGACGACGACTTTGAGCTCGAAGGGTAT
CTCGAGGGCATCGAGCGCGACCTCATCGAACGCGCCCTGGAGAAGACGAGATACAACAAGACCGCCGCCGCCCAGCTCCT
CGGCATCAGCTTCCGCGCTCTCAGATACCGCCTGAAGAAGCTGAACATGGAGTAG

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(134-300)

Bacterial regulatory protein, Fis family (HTH_8)

(401-441)

Response regulator receiver domain (Response_reg)

(7-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U2N0M9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

67.184

100

0.682
  pilR Acinetobacter baumannii strain A118

48.608

100

0.511
  luxO Vibrio cholerae strain A1552

37.668

100

0.378