Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   LJU29_RS22830 Genome accession   NZ_CP085684
Coordinates   5145389..5145805 (-) Length   138 a.a.
NCBI ID   WP_122322339.1    Uniprot ID   -
Organism   Pseudomonas ficuserectae strain ZJDX-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5140389..5150805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJU29_RS22810 - 5140695..5141693 (-) 999 WP_122322330.1 ATP-grasp domain-containing protein -
  LJU29_RS22815 - 5141813..5142358 (+) 546 WP_011167731.1 DUF1523 family protein -
  LJU29_RS22820 - 5142408..5143694 (-) 1287 WP_122322328.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  LJU29_RS22825 - 5143806..5145227 (-) 1422 WP_371321921.1 O-antigen ligase family protein -
  LJU29_RS22830 pilA2 5145389..5145805 (-) 417 WP_122322339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LJU29_RS22835 pilB 5146030..5147724 (+) 1695 WP_122322326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LJU29_RS22840 pilC 5147727..5148944 (+) 1218 WP_122322325.1 type II secretion system F family protein Machinery gene
  LJU29_RS22845 pilD 5148946..5149818 (+) 873 WP_122322324.1 A24 family peptidase Machinery gene
  LJU29_RS22850 coaE 5149815..5150438 (+) 624 WP_005745478.1 dephospho-CoA kinase -
  LJU29_RS22855 yacG 5150435..5150644 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14163.35 Da        Isoelectric Point: 8.1070

>NTDB_id=619293 LJU29_RS22830 WP_122322339.1 5145389..5145805(-) (pilA2) [Pseudomonas ficuserectae strain ZJDX-003]
MQTQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKASELILAASSARTCVTETVASKGFLALDACGTGFVATTYAKS
LTVNATTGAITVKGFIKAADDTTVTMTPTKNAGGTDITGWTCIGTPSNWMPGSCKSNS

Nucleotide


Download         Length: 417 bp        

>NTDB_id=619293 LJU29_RS22830 WP_122322339.1 5145389..5145805(-) (pilA2) [Pseudomonas ficuserectae strain ZJDX-003]
ATGCAAACGCAAAAAGGCTTTACGTTGATCGAATTGATGATCGTTGTTGCGATCATCGGAATTTTGGCTGCTGTCGCACT
ACCCGCTTATCAGGACTACACTGCGCGAGCAAAAGCTTCGGAGCTGATCCTGGCTGCGAGTAGCGCCCGTACGTGCGTGA
CTGAGACCGTCGCCAGCAAGGGGTTTCTTGCGCTCGACGCATGTGGCACAGGATTCGTCGCTACTACATATGCGAAAAGC
TTGACTGTCAACGCGACCACCGGGGCAATTACTGTGAAAGGCTTCATTAAAGCGGCGGATGACACAACTGTCACTATGAC
GCCCACTAAAAACGCTGGTGGCACAGACATTACCGGCTGGACGTGTATCGGTACTCCTTCCAATTGGATGCCAGGCTCTT
GCAAGTCAAACAGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

48.905

99.275

0.486
  pilA2 Legionella pneumophila str. Paris

48.905

99.275

0.486
  pilA/pilA1 Eikenella corrodens VA1

43.333

100

0.471
  comP Acinetobacter baylyi ADP1

41.611

100

0.449
  pilA Haemophilus influenzae 86-028NP

40.972

100

0.428
  pilA Haemophilus influenzae Rd KW20

38.776

100

0.413
  pilA Ralstonia pseudosolanacearum GMI1000

42.748

94.928

0.406
  pilE Neisseria gonorrhoeae strain FA1090

42.308

94.203

0.399
  pilA Acinetobacter baumannii strain A118

38.194

100

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.589

100

0.384