Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LJU29_RS22840 Genome accession   NZ_CP085684
Coordinates   5147727..5148944 (+) Length   405 a.a.
NCBI ID   WP_122322325.1    Uniprot ID   A0A3M5J9T5
Organism   Pseudomonas ficuserectae strain ZJDX-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5142727..5153944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJU29_RS22825 - 5143806..5145227 (-) 1422 WP_371321921.1 O-antigen ligase family protein -
  LJU29_RS22830 pilA2 5145389..5145805 (-) 417 WP_122322339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LJU29_RS22835 pilB 5146030..5147724 (+) 1695 WP_122322326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LJU29_RS22840 pilC 5147727..5148944 (+) 1218 WP_122322325.1 type II secretion system F family protein Machinery gene
  LJU29_RS22845 pilD 5148946..5149818 (+) 873 WP_122322324.1 A24 family peptidase Machinery gene
  LJU29_RS22850 coaE 5149815..5150438 (+) 624 WP_005745478.1 dephospho-CoA kinase -
  LJU29_RS22855 yacG 5150435..5150644 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  LJU29_RS22860 - 5150842..5151126 (-) 285 Protein_4526 hypothetical protein -
  LJU29_RS22865 - 5151241..5151870 (+) 630 WP_003430735.1 DUF1780 domain-containing protein -
  LJU29_RS22870 - 5151867..5152400 (+) 534 WP_122322321.1 MOSC domain-containing protein -
  LJU29_RS29760 - 5152336..5152521 (+) 186 WP_324608203.1 DUF3094 family protein -
  LJU29_RS22875 - 5152618..5153916 (+) 1299 WP_011167718.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44038.84 Da        Isoelectric Point: 10.0247

>NTDB_id=619295 LJU29_RS22840 WP_122322325.1 5147727..5148944(+) (pilC) [Pseudomonas ficuserectae strain ZJDX-003]
MASKAVKVTVYSWEGVDKKGTKISGELSGHNLALVKAQLRKQGINPTKVRKKSVSLFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIIEGSENPNMQALVNSLKQEVSAGNSFAAALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQSVFTGFGAELPGFTLMVIGLSEIVQQWWLGLVGLFFVGFFIFK
KAYKQSQKFRDSMDRFLLKTPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRHAVNQVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGSAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=619295 LJU29_RS22840 WP_122322325.1 5147727..5148944(+) (pilC) [Pseudomonas ficuserectae strain ZJDX-003]
ATGGCCAGCAAGGCAGTTAAAGTCACCGTTTACAGCTGGGAAGGTGTCGATAAAAAAGGCACTAAAATTAGTGGCGAATT
GAGTGGCCATAATCTGGCGCTCGTAAAAGCTCAACTGCGCAAGCAAGGCATCAATCCGACCAAGGTGCGCAAGAAGTCCG
TATCGTTATTCGGCAAGGGCAAGAAGATAAAGCCGCTGGACATCGCGTTCTTTTCTCGGCAGATGGCGACAATGATGAAG
GCGGGGGTGCCGCTGCTACAATCATTTGACATCATCATCGAGGGCAGCGAAAACCCTAACATGCAAGCGCTGGTAAATTC
CCTCAAACAAGAGGTGTCTGCCGGCAATAGTTTCGCAGCAGCCTTGAGGCAGAAGCCTGAATATTTCGACGACTTGTTTT
GCAACTTAGTAGACGCTGGGGAGCAGGCTGGTGCGCTAGAGAGTCTGCTAGACCGAGTGGCGAGCTATAAGGAAAAGACC
GAAAAGCTGAAAGCCAAAATTAAAAAAGCCATGACTTATCCCGCTGCCGTTTTGATAGTAGCCATTATTGTTTCCGGCAT
CTTATTGATCAAGGTAGTGCCGCAATTTCAATCTGTATTCACCGGCTTTGGCGCGGAGCTGCCGGGGTTCACATTGATGG
TCATCGGCCTATCTGAAATCGTACAACAGTGGTGGCTAGGGCTAGTTGGCTTATTTTTTGTCGGGTTCTTCATATTTAAA
AAAGCTTATAAGCAGTCTCAGAAATTTCGAGACAGCATGGATCGTTTTCTACTGAAAACACCTATCATTGGACCATTGAT
TTTTAAATCGTCAGTGGCCCGTTATGCTCGGACACTTGCTACAACTTTTGCCGCTGGCGTGCCGCTTGTCGAAGCGTTGG
ACTCTGTCGCCGGAGCCACGGGTAACGTAGTTTTTAGACATGCGGTCAATCAGGTCAAACAGGATGTCTCCACAGGTATG
CAATTGAATTTTTCGATGCGCTCTACCGGCGTTTTCCCGAGTCTGGCAATCCAAATGACTGCCATTGGCGAGGAGTCCGG
CGCATTAGACAATATGCTGGACAAAGTAGCGACCTACTACGAGGATGAAGTCGACAATATGGTAGATAGCCTGACCAGCC
TGATGGAACCGATGATCATGGCCGTGCTGGGCGTTATAGTAGGCGGCTTAGTAATTGCGATGTACCTCCCCATCTTCAAA
CTTGGAAGCGCGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M5J9T5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.416

99.753

0.583
  pilC Legionella pneumophila strain ERS1305867

52.525

97.778

0.514
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

42.26

100

0.425
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Thermus thermophilus HB27

38.653

99.012

0.383