NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	LJU29_RS22845	Genome accession	NZ_CP085684
Coordinates	5148946..5149818 (+)	Length	290 a.a.
NCBI ID	WP_122322324.1	Uniprot ID	-
Organism	Pseudomonas ficuserectae strain ZJDX-003
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5143946..5154818

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LJU29_RS22830	pilA2	5145389..5145805 (-)	417	WP_122322339.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
LJU29_RS22835	pilB	5146030..5147724 (+)	1695	WP_122322326.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LJU29_RS22840	pilC	5147727..5148944 (+)	1218	WP_122322325.1	type II secretion system F family protein	Machinery gene
LJU29_RS22845	pilD	5148946..5149818 (+)	873	WP_122322324.1	A24 family peptidase	Machinery gene
LJU29_RS22850	coaE	5149815..5150438 (+)	624	WP_005745478.1	dephospho-CoA kinase	-
LJU29_RS22855	yacG	5150435..5150644 (+)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
LJU29_RS22860	-	5150842..5151126 (-)	285	Protein_4526	hypothetical protein	-
LJU29_RS22865	-	5151241..5151870 (+)	630	WP_003430735.1	DUF1780 domain-containing protein	-
LJU29_RS22870	-	5151867..5152400 (+)	534	WP_122322321.1	MOSC domain-containing protein	-
LJU29_RS29760	-	5152336..5152521 (+)	186	WP_324608203.1	DUF3094 family protein	-
LJU29_RS22875	-	5152618..5153916 (+)	1299	WP_011167718.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

Download Length: 290 a.a. Molecular weight: 32150.33 Da Isoelectric Point: 8.3769

                                >NTDB_id=619296 LJU29_RS22845 WP_122322324.1 5148946..5149818(+) (pilD) [Pseudomonas ficuserectae strain ZJDX-003]

MPLLDFLASSPLAFVITCCILGLIIGSFLNVVVYRLPRMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR

PWENLPVVSYLLLRGKCSHCKAPISMRYPMVELTCAVLSAYVAWHFGFGWQTAAMLVLSWGLLAMSLIDADHQLLPDSLV

LPLLWLGLIVNAFGLFTSLNDSLWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG

AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

Download Length: 873 bp

                                >NTDB_id=619296 LJU29_RS22845 WP_122322324.1 5148946..5149818(+) (pilD) [Pseudomonas ficuserectae strain ZJDX-003]

ATGCCCCTCCTCGATTTCCTGGCCAGTTCCCCCCTGGCCTTCGTCATTACCTGCTGCATCCTCGGCCTCATCATCGGAAG

CTTCCTCAACGTAGTCGTCTACCGCCTCCCCAGAATGATGGAGCGCGACTGGAAAGCCCAATCCCGTGAAATGCTCGGCC

TGCCCGCCGAACCTGATCAGCCTGCCTTCAACCTGAACCGCCCCCGCTCCAGTTGCCCGCACTGCGCGCACAAAATCCGC

CCGTGGGAAAACCTGCCGGTCGTCAGCTATCTGCTGCTGCGCGGTAAATGCTCACACTGCAAAGCCCCCATTAGCATGCG

CTACCCAATGGTAGAGCTGACCTGCGCAGTGTTGTCGGCCTACGTCGCCTGGCATTTCGGTTTCGGCTGGCAAACGGCGG

CAATGCTGGTGTTGAGCTGGGGCCTGCTGGCGATGAGTCTGATCGACGCCGACCACCAGCTGTTACCTGACTCTCTGGTG

CTGCCACTGTTGTGGCTGGGCCTCATCGTCAACGCTTTCGGGCTGTTCACATCGCTGAACGATTCGCTGTGGGGCGCGGT

GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTATTCAAACTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT

TCAAGTTGCTGGCCATGCTTGGCGCATGGGGCGGCTGGCAGATTCTGCCGTTGACCATCCTGCTGTCATCGCTGGTGGGT

GCGGTGCTTGGGGTGATCATGATGCGCGTGCGCAGGGTTGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT

TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(136-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio campbellii strain DS40M4	52.297	97.586	0.51
pilD	Vibrio cholerae strain A1552	53.623	95.172	0.51
pilD	Acinetobacter baumannii D1279779	51.056	97.931	0.5
pilD	Acinetobacter nosocomialis M2	50.704	97.931	0.497
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472

Detailed information NTG0619296