Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LJU29_RS22835 Genome accession   NZ_CP085684
Coordinates   5146030..5147724 (+) Length   564 a.a.
NCBI ID   WP_122322326.1    Uniprot ID   -
Organism   Pseudomonas ficuserectae strain ZJDX-003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5141030..5152724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJU29_RS22815 - 5141813..5142358 (+) 546 WP_011167731.1 DUF1523 family protein -
  LJU29_RS22820 - 5142408..5143694 (-) 1287 WP_122322328.1 polysaccharide biosynthesis C-terminal domain-containing protein -
  LJU29_RS22825 - 5143806..5145227 (-) 1422 WP_371321921.1 O-antigen ligase family protein -
  LJU29_RS22830 pilA2 5145389..5145805 (-) 417 WP_122322339.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LJU29_RS22835 pilB 5146030..5147724 (+) 1695 WP_122322326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LJU29_RS22840 pilC 5147727..5148944 (+) 1218 WP_122322325.1 type II secretion system F family protein Machinery gene
  LJU29_RS22845 pilD 5148946..5149818 (+) 873 WP_122322324.1 A24 family peptidase Machinery gene
  LJU29_RS22850 coaE 5149815..5150438 (+) 624 WP_005745478.1 dephospho-CoA kinase -
  LJU29_RS22855 yacG 5150435..5150644 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  LJU29_RS22860 - 5150842..5151126 (-) 285 Protein_4526 hypothetical protein -
  LJU29_RS22865 - 5151241..5151870 (+) 630 WP_003430735.1 DUF1780 domain-containing protein -
  LJU29_RS22870 - 5151867..5152400 (+) 534 WP_122322321.1 MOSC domain-containing protein -
  LJU29_RS29760 - 5152336..5152521 (+) 186 WP_324608203.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62495.82 Da        Isoelectric Point: 6.1636

>NTDB_id=619294 LJU29_RS22835 WP_122322326.1 5146030..5147724(+) (pilB) [Pseudomonas ficuserectae strain ZJDX-003]
MTDVVLTGLAKQLVLAELLTEIAAQQAYQQARREKISLVSYLVQNKLVKSLTLAEIASDQFGVPFVDLSSLDKESQPKGL
VSEKLVRQHNALPLWRRGNKLFIGISDPTNHQAITDIQFSTGLNTEAILVEDDKLSLAIDRFFDTESGLGELADIDLGLE
IEPASDKETSLATQSDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKNKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKALYMEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGTFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPALERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=619294 LJU29_RS22835 WP_122322326.1 5146030..5147724(+) (pilB) [Pseudomonas ficuserectae strain ZJDX-003]
ATGACTGATGTTGTTCTCACCGGTTTGGCCAAGCAACTGGTCTTGGCCGAGCTTCTTACAGAAATTGCCGCCCAGCAGGC
CTATCAGCAGGCGCGCCGCGAAAAAATTTCGCTGGTTAGCTATCTGGTACAAAACAAACTGGTCAAAAGCCTTACGCTGG
CAGAGATCGCGTCTGATCAGTTCGGCGTTCCTTTCGTGGATCTTTCCAGTCTTGACAAAGAAAGCCAGCCTAAGGGGTTG
GTGAGTGAGAAGCTCGTCAGACAGCATAATGCCCTCCCCCTGTGGCGCCGTGGTAACAAGCTGTTTATCGGCATATCGGA
CCCGACAAACCATCAGGCGATCACAGATATTCAGTTCAGCACTGGCCTGAATACTGAAGCGATTCTGGTTGAAGATGACA
AGCTAAGCCTTGCCATAGACCGCTTTTTTGACACTGAAAGCGGCCTCGGCGAGTTGGCGGATATTGATCTGGGTCTTGAA
ATCGAACCTGCCAGTGACAAGGAAACCTCACTGGCGACACAGAGTGATGCCGACGATGCTCCTGTTGTGCGCTTCGTCAA
CAAGATGCTGATGGATGCGATACGGCTGGGCTCGTCGGACCTGCATTTTGAACCTTACGAAAAGATTTTTCGCGTGCGCC
TGCGTACCGACGGCATTTTGCATGAAGTGGCCAGACCACCGATTCATCTGGCCAATCGGATTGCCGCACGGCTTAAGGTC
ATGGCCAGTCTCGATATCTCTGAGCGGCGTAAACCGCAGGACGGTAGGGTCAAGCTGCGAGTGTCAAAAAACAAAGCTAT
CGACTTCCGTATGAACACGCTGCCGACACTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATCCGACCAGCGCACAGA
TGGGCATTGACGCGCTGGGCTATGAGCCGGACCAAAAAGCTTTATATATGGAGGCCTTGAAACAGCCACAAGGCATGATT
CTTGTGACCGGCCCCACTGGCTCGGGTAAAACCGTCTCGCTGTACACTGGCCTGAACATTCTTAATACTGTGGACATCAA
CATTTCCACAGCTGAAGACCCGGTAGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTCAATCCGCGTCAGGGATTGG
ACTTTTCCCAGGCATTGCGCGCATTCCTGCGTCAAGATCCCGACGTAATCATGGTCGGCGAGATACGAGATCTGGAAACC
GCCGAAATCGCCATTAAAGCCTCGCAGACCGGCCACATGGTGTTATCTACTCTGCATACTAACAGCGCCGCAGAAACTCT
GACTCGCTTGCATCATATGGGTGTCGCAGCTTTCAACATCGCTACAGCAATCAACCTCATCATTGCCCAGCGGCTGGCGC
GCAAGCTGTGCAGCCATTGTAAGAAAGAACTCGATATACCCCGGGAAACGCTCATCCAGGAGGGTTTTCCGGAGGAGAAG
ATCGGTACGTTTAAAATCTATGGCCCAGTAGGGTGTGAGCATTGCAACGGCGGCTACAGGGGCAGGGTCGGGATTTATGA
AGTGGTCAAGAAAACACCTGCGCTGGAACGTATCATCATGGAAGAAGGCAATTCACTGGAGATTTCAAGGCAGATGCGCA
AGGACGGCTTCAATGATTTGAGGACCTCCGGCCTGTCGAAGGCCATGCAAGGCATTACCAGTCTTGAAGAAGTCAATCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

100

0.585
  pilB Acinetobacter baylyi ADP1

57.295

99.645

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

100

0.56
  pilB Vibrio cholerae strain A1552

50.624

99.468

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

51.083

98.227

0.502
  pilB Vibrio campbellii strain DS40M4

49.291

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.128

99.468

0.479
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.469

93.44

0.369