Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO77_RS06910 Genome accession   NZ_CP085465
Coordinates   1515515..1516654 (-) Length   379 a.a.
NCBI ID   WP_005361600.1    Uniprot ID   -
Organism   Aeromonas veronii strain 71506     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1510515..1521654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO77_RS06890 (LEO77_06890) oppF 1511265..1512260 (+) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  LEO77_RS06895 (LEO77_06895) - 1512563..1513507 (+) 945 WP_227342375.1 TerC family protein -
  LEO77_RS06900 (LEO77_06900) alr 1513572..1514798 (-) 1227 WP_040067860.1 alanine racemase -
  LEO77_RS06905 (LEO77_06905) - 1514972..1515421 (+) 450 WP_019446653.1 isoprenylcysteine carboxylmethyltransferase family protein -
  LEO77_RS06910 (LEO77_06910) pilU 1515515..1516654 (-) 1140 WP_005361600.1 type IVa pilus ATPase TapW Machinery gene
  LEO77_RS06915 (LEO77_06915) - 1516713..1517069 (-) 357 WP_005353120.1 DMT family protein -
  LEO77_RS06920 (LEO77_06920) - 1517182..1517914 (+) 733 Protein_1326 substrate-binding periplasmic protein -
  LEO77_RS06925 (LEO77_06925) btuC 1518014..1519021 (+) 1008 WP_227342376.1 vitamin B12 ABC transporter permease BtuC -
  LEO77_RS06930 (LEO77_06930) - 1519075..1519872 (+) 798 WP_227342378.1 ATP-binding cassette domain-containing protein -
  LEO77_RS06935 (LEO77_06935) - 1519989..1521050 (-) 1062 WP_064336455.1 3-deoxy-7-phosphoheptulonate synthase -
  LEO77_RS06940 (LEO77_06940) - 1521195..1521575 (+) 381 WP_005337641.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42239.31 Da        Isoelectric Point: 6.6009

>NTDB_id=618183 LEO77_RS06910 WP_005361600.1 1515515..1516654(-) (pilU) [Aeromonas veronii strain 71506]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTDIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=618183 LEO77_RS06910 WP_005361600.1 1515515..1516654(-) (pilU) [Aeromonas veronii strain 71506]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAGCCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGACATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGTTGCTCGACACCATCATGGAGAAACGCGGTCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGTAAATCCACTTCGCTGGCGGCACTTATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATTACCATCGAGGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTTGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTCAAAGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTTGATCTGGTGGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGTTGTGGAAGGAGAAGGGGCAGATTGCCAGCAGCAGCGATGCCACTGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367