Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LF296_RS04820 Genome accession   NZ_CP085083
Coordinates   1004630..1005748 (-) Length   372 a.a.
NCBI ID   WP_272655644.1    Uniprot ID   A0AAJ6NKK7
Organism   Acinetobacter vivianii strain KJ-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 999630..1010748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LF296_RS04790 (LF296_04790) - 1000293..1001141 (+) 849 WP_032863849.1 class II glutamine amidotransferase -
  LF296_RS04795 (LF296_04795) - 1001425..1001880 (+) 456 WP_004770972.1 bacteriohemerythrin -
  LF296_RS04800 (LF296_04800) - 1002127..1003209 (+) 1083 WP_272655643.1 hypothetical protein -
  LF296_RS04805 (LF296_04805) - 1003209..1003529 (+) 321 WP_005256404.1 RnfH family protein -
  LF296_RS04810 (LF296_04810) - 1003586..1003984 (-) 399 WP_004655148.1 outer membrane protein assembly factor BamE -
  LF296_RS04815 (LF296_04815) fur 1004096..1004533 (+) 438 WP_004770976.1 ferric iron uptake transcriptional regulator -
  LF296_RS04820 (LF296_04820) pilU 1004630..1005748 (-) 1119 WP_272655644.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LF296_RS04825 (LF296_04825) pilT 1005773..1006810 (-) 1038 WP_004770978.1 type IV pilus twitching motility protein PilT Machinery gene
  LF296_RS04830 (LF296_04830) - 1006937..1007629 (+) 693 WP_272655645.1 YggS family pyridoxal phosphate-dependent enzyme -
  LF296_RS04835 (LF296_04835) - 1007626..1008210 (-) 585 WP_272655646.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41761.90 Da        Isoelectric Point: 6.1349

>NTDB_id=615112 LF296_RS04820 WP_272655644.1 1004630..1005748(-) (pilU) [Acinetobacter vivianii strain KJ-1]
MDFNDLLNLMIQQNASDLFVTADVEPSMKINGQIVPVSKTKLSGEIVDQLVQSIMTDKQRKEFADTRECNFAIMNHEKTA
RFRVSAFQQRDMPGMVLRKIETHIPTMDDLKLPEVLKELAMTKRGIIIFVGATGTGKSTSLASMIGFRNQNSKGHIITIE
DPIEFVHQHAGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFEADRHSQLYMDLSLNLKALVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLSEEGADQLLSASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=615112 LF296_RS04820 WP_272655644.1 1004630..1005748(-) (pilU) [Acinetobacter vivianii strain KJ-1]
ATGGACTTTAATGATTTACTCAATTTGATGATTCAACAGAATGCCTCGGACTTATTTGTCACAGCAGACGTTGAACCTTC
AATGAAAATTAACGGGCAGATTGTACCTGTTTCAAAAACCAAGCTGTCAGGGGAAATTGTTGATCAACTAGTACAGTCCA
TCATGACTGACAAACAGCGTAAAGAATTTGCAGATACACGCGAATGTAACTTTGCGATCATGAACCATGAAAAGACCGCA
CGTTTCCGTGTCAGTGCCTTTCAGCAACGTGATATGCCGGGCATGGTGTTACGTAAAATCGAAACCCATATTCCAACCAT
GGATGACCTGAAACTGCCTGAAGTTCTGAAAGAACTGGCGATGACCAAACGCGGCATCATCATTTTTGTAGGGGCAACAG
GTACAGGTAAGTCGACCTCCCTCGCTTCAATGATTGGTTTCCGCAACCAGAATTCTAAAGGTCATATTATTACCATTGAA
GATCCGATCGAATTTGTGCATCAACATGCAGGTTGCATTGTGACACAACGCGAAGTGGGCATTGATACCGATTCATTTGA
AGTGGCATTGAAAAATACGCTACGTCAGGCACCAGATGTGATCTTGATTGGTGAGATTCGCTCTCGTGAAACTATGGACT
ATGCGATTGCCTTCGCAGAAACTGGTCACTTGGTACTCGCGACCCTGCACGCCAACAATGCCAACCAAGCCTTAGACCGC
ATTATTCACTTCTTTGAAGCTGATCGTCATAGCCAGCTGTATATGGACTTGTCATTGAACTTAAAAGCATTGGTGGCACA
ACAGTTAATTCCAACGCCTGACGGCAATTCGCGTCGTGCTGCGATTGAGATTCTGATTAACACCCCGCTTCTGGCCGACT
ATATCCGTAAAGGTGAAATTCATGAGATTAAAGATCTGATGAAACGTTCACGCGAGCTAGGCATGCAGACCTTCGATCAG
GCTTTATTTGACCTTTATAAAGCAGGTCAGATTACCTATAAGGATGCATTGAAGCATGCAGACTCACCAAACGATCTACG
TCTCACCATCAAACTTTCAGAAGAAGGCGCAGACCAGTTATTGAGTGCCAGCCGCAACATTACCTTTGATGGTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

70.35

99.731

0.702
  pilU Vibrio cholerae strain A1552

54.324

99.462

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

97.849

0.398
  pilT Legionella pneumophila strain ERS1305867

40.476

90.323

0.366
  pilT Legionella pneumophila strain Lp02

40.476

90.323

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.017

93.011

0.363
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36