Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LCH17_RS03585 Genome accession   NZ_CP083944
Coordinates   830193..831332 (+) Length   379 a.a.
NCBI ID   WP_139392833.1    Uniprot ID   A0AAD3YMJ9
Organism   Aeromonas hydrophila strain CSUSB2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825193..836332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH17_RS03555 (LCH17_03555) - 825500..826564 (+) 1065 WP_011706545.1 3-deoxy-7-phosphoheptulonate synthase -
  LCH17_RS03560 (LCH17_03560) aac(6') 826571..827029 (+) 459 WP_224480762.1 aminoglycoside 6'-N-acetyltransferase -
  LCH17_RS03565 (LCH17_03565) - 827051..827836 (-) 786 WP_224480763.1 ATP-binding cassette domain-containing protein -
  LCH17_RS03570 (LCH17_03570) btuC 827823..828830 (-) 1008 WP_206818117.1 vitamin B12 ABC transporter permease BtuC -
  LCH17_RS03575 (LCH17_03575) - 828931..829662 (-) 732 WP_029300033.1 ABC transporter substrate-binding protein -
  LCH17_RS03580 (LCH17_03580) - 829776..830132 (+) 357 WP_005302630.1 DMT family protein -
  LCH17_RS03585 (LCH17_03585) pilU 830193..831332 (+) 1140 WP_139392833.1 type IVa pilus ATPase TapW Machinery gene
  LCH17_RS03590 (LCH17_03590) glgC 831615..832886 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  LCH17_RS03595 (LCH17_03595) - 833022..833501 (+) 480 WP_224480764.1 WbuC family cupin fold metalloprotein -
  LCH17_RS03600 (LCH17_03600) - 833603..833845 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  LCH17_RS03605 (LCH17_03605) feoB 833842..836115 (-) 2274 WP_224480765.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42278.35 Da        Isoelectric Point: 6.4290

>NTDB_id=608717 LCH17_RS03585 WP_139392833.1 830193..831332(+) (pilU) [Aeromonas hydrophila strain CSUSB2]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=608717 LCH17_RS03585 WP_139392833.1 830193..831332(+) (pilU) [Aeromonas hydrophila strain CSUSB2]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAATGGCGGCCTGCGTCCGCTCAGTGAAACGCCGCTCGAGCCCGGCGAGGTAGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGACGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGATACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACTATCGAAGAT
CCGGTGGAGTTCGTGCACCGTCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCACCCGATGTCATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACTACCGATGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTTGTCGAGGGCGTGATTGATGAGGAGGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGATG
AGAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361