Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA/pilAI   Type   Machinery gene
Locus tag   K8O61_RS04555 Genome accession   NZ_CP083804
Coordinates   1051285..1051692 (+) Length   135 a.a.
NCBI ID   WP_237655666.1    Uniprot ID   -
Organism   Xanthomonas cerealis pv. cerealis strain ICMP 16317     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1051757..1071297 1051285..1051692 flank 65


Gene organization within MGE regions


Location: 1051285..1071297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O61_RS04555 (K8O61_04570) pilA/pilAI 1051285..1051692 (+) 408 WP_237655666.1 pilin Machinery gene
  K8O61_RS04565 (K8O61_04580) - 1051781..1052167 (+) 387 WP_272929860.1 pilin -
  K8O61_RS04570 (K8O61_04585) - 1052319..1054067 (+) 1749 WP_237655667.1 hypothetical protein -
  K8O61_RS04575 (K8O61_04590) - 1054280..1055569 (+) 1290 WP_237655668.1 lipopolysaccharide biosynthesis protein -
  K8O61_RS04580 (K8O61_04595) - 1055566..1056354 (+) 789 WP_237655669.1 class I SAM-dependent methyltransferase -
  K8O61_RS04585 (K8O61_04600) - 1056463..1057506 (-) 1044 WP_237655670.1 glycosyltransferase family 4 protein -
  K8O61_RS04590 (K8O61_04605) - 1057478..1058110 (-) 633 WP_237655671.1 class I SAM-dependent methyltransferase -
  K8O61_RS04595 (K8O61_04610) - 1058221..1059327 (-) 1107 WP_237655672.1 glycosyltransferase family 4 protein -
  K8O61_RS04600 (K8O61_04615) - 1059380..1060237 (-) 858 WP_237655673.1 glycosyltransferase family 2 protein -
  K8O61_RS04605 (K8O61_04620) pilB 1060389..1062125 (+) 1737 WP_237655674.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K8O61_RS04610 (K8O61_04625) - 1062135..1062752 (-) 618 WP_237655675.1 Fic/DOC family protein -
  K8O61_RS04615 (K8O61_04630) - 1062749..1062931 (-) 183 WP_003466380.1 YhfG family protein -
  K8O61_RS04620 (K8O61_04635) pilR 1063120..1064502 (-) 1383 WP_237655676.1 sigma-54-dependent transcriptional regulator Regulator
  K8O61_RS04625 (K8O61_04640) - 1064576..1066189 (-) 1614 WP_039008924.1 sensor histidine kinase -
  K8O61_RS04630 (K8O61_04645) sucC 1066431..1067594 (+) 1164 WP_053833739.1 ADP-forming succinate--CoA ligase subunit beta -
  K8O61_RS04635 (K8O61_04650) sucD 1067618..1068490 (+) 873 WP_009580520.1 succinate--CoA ligase subunit alpha -
  K8O61_RS04640 (K8O61_04655) - 1068923..1069372 (+) 450 WP_237656120.1 hypothetical protein -
  K8O61_RS04645 (K8O61_04660) - 1069441..1071297 (-) 1857 WP_155521865.1 hypothetical protein -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 14368.52 Da        Isoelectric Point: 9.2502

>NTDB_id=607988 K8O61_RS04555 WP_237655666.1 1051285..1051692(+) (pilA/pilAI) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVRKSQVTAALSDITPGKTQFEVLTNEGKSITSATDIGLTAATSRCSE
IKPTAGDNGNINCKMIGGAGVQGQEIRWTRNASGNWTCSTSAIEKYRPSTCNKAL

Nucleotide


Download         Length: 408 bp        

>NTDB_id=607988 K8O61_RS04555 WP_237655666.1 1051285..1051692(+) (pilA/pilAI) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGAAGAAGCAGCAGGGCTTTACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGGCCTACCAGAACTACGTTCGCAAGTCGCAGGTCACCGCGGCGCTGTCCGACATCACCCCGGGCAAGACGCAGTTCG
AAGTGCTGACCAACGAAGGCAAGAGCATCACCAGCGCGACCGATATCGGTCTTACTGCAGCGACCAGTCGTTGCAGCGAG
ATTAAGCCGACCGCAGGGGACAACGGCAATATCAATTGCAAAATGATCGGCGGCGCTGGTGTGCAGGGCCAGGAAATTCG
TTGGACACGCAACGCCTCCGGCAACTGGACTTGTTCGACCAGTGCTATCGAGAAGTATCGTCCGAGCACCTGCAACAAGG
CGCTGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

53.333

100

0.533

  pilA Pseudomonas aeruginosa PAK

42

100

0.467

  pilA Acinetobacter baumannii strain A118

44.853

100

0.452

  pilA Vibrio cholerae C6706

41.259

100

0.437

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.259

100

0.437

  pilA Vibrio cholerae strain A1552

41.259

100

0.437

  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.105

98.519

0.415

  pilA2 Legionella pneumophila str. Paris

40

100

0.4

  pilA2 Legionella pneumophila strain ERS1305867

40

100

0.4

  comP Acinetobacter baylyi ADP1

35.57

100

0.393

  pilA Vibrio parahaemolyticus RIMD 2210633

38.889

93.333

0.363