Detailed information NTG0607989

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: K8O61_RS04605
• Genome accession: NZ_CP083804
• Coordinates: 1060389..1062125 (+)
• Length: 578 a.a.
• NCBI ID: WP_237655674.1
• Uniprot ID: -
• Organism: Xanthomonas cerealis pv. cerealis strain ICMP 16317
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1051757..1071297	1060389..1062125	within	0

Gene organization within MGE regions

Location: 1051757..1071297

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8O61_RS04565 (K8O61_04580)	-	1051781..1052167 (+)	387	WP_272929860.1	pilin	-
K8O61_RS04570 (K8O61_04585)	-	1052319..1054067 (+)	1749	WP_237655667.1	hypothetical protein	-
K8O61_RS04575 (K8O61_04590)	-	1054280..1055569 (+)	1290	WP_237655668.1	lipopolysaccharide biosynthesis protein	-
K8O61_RS04580 (K8O61_04595)	-	1055566..1056354 (+)	789	WP_237655669.1	class I SAM-dependent methyltransferase	-
K8O61_RS04585 (K8O61_04600)	-	1056463..1057506 (-)	1044	WP_237655670.1	glycosyltransferase family 4 protein	-
K8O61_RS04590 (K8O61_04605)	-	1057478..1058110 (-)	633	WP_237655671.1	class I SAM-dependent methyltransferase	-
K8O61_RS04595 (K8O61_04610)	-	1058221..1059327 (-)	1107	WP_237655672.1	glycosyltransferase family 4 protein	-
K8O61_RS04600 (K8O61_04615)	-	1059380..1060237 (-)	858	WP_237655673.1	glycosyltransferase family 2 protein	-
K8O61_RS04605 (K8O61_04620)	pilB	1060389..1062125 (+)	1737	WP_237655674.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K8O61_RS04610 (K8O61_04625)	-	1062135..1062752 (-)	618	WP_237655675.1	Fic/DOC family protein	-
K8O61_RS04615 (K8O61_04630)	-	1062749..1062931 (-)	183	WP_003466380.1	YhfG family protein	-
K8O61_RS04620 (K8O61_04635)	pilR	1063120..1064502 (-)	1383	WP_237655676.1	sigma-54-dependent transcriptional regulator	Regulator
K8O61_RS04625 (K8O61_04640)	-	1064576..1066189 (-)	1614	WP_039008924.1	sensor histidine kinase	-
K8O61_RS04630 (K8O61_04645)	sucC	1066431..1067594 (+)	1164	WP_053833739.1	ADP-forming succinate--CoA ligase subunit beta	-
K8O61_RS04635 (K8O61_04650)	sucD	1067618..1068490 (+)	873	WP_009580520.1	succinate--CoA ligase subunit alpha	-
K8O61_RS04640 (K8O61_04655)	-	1068923..1069372 (+)	450	WP_237656120.1	hypothetical protein	-
K8O61_RS04645 (K8O61_04660)	-	1069441..1071297 (-)	1857	WP_155521865.1	hypothetical protein	-

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62818.06 Da
• Isoelectric Point: 6.2649

>NTDB_id=607989 K8O61_RS04605 WP_237655674.1 1060389..1062125(+) (pilB) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MNAAVSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGNSLG
DDDEGMGNLEVGVGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNTCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=607989 K8O61_RS04605 WP_237655674.1 1060389..1062125(+) (pilB) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGAACGCAGCTGTCTCGGCCAACCTGGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCGAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTGGGGGTCAGCAATCCGACCCAGACTCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGAGCAACGATGCGATCGGCAACAGCCTGGGT
GACGACGACGAGGGCATGGGCAATCTCGAGGTCGGTGTCGGCGACGAAGACATGGGCAGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCTGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAATCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACGGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCGGAGATTGCGATCAAGGCGGCGCAGACCGGGCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGGTTGTGCAACACCTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGTGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCGCGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (201-468)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (51-152)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.106	98.27	0.542
pilB	Acinetobacter baumannii D1279779	54.641	98.789	0.54
pilB	Legionella pneumophila strain ERS1305867	52.548	98.443	0.517
pilB	Vibrio cholerae strain A1552	49.393	99.827	0.493
pilF	Neisseria gonorrhoeae MS11	49.12	98.27	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.316	98.616	0.457
pilB	Vibrio campbellii strain DS40M4	45.55	99.135	0.452
pilF	Thermus thermophilus HB27	40.038	90.311	0.362