Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LA327_RS13425 Genome accession   NZ_CP083622
Coordinates   2717665..2718708 (-) Length   347 a.a.
NCBI ID   WP_008791315.1    Uniprot ID   A0A9Q2WWJ4
Organism   Thomasclavelia ramosa strain FDAARGOS_1519     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2712665..2723708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA327_RS13405 (LA327_13405) - 2713502..2714707 (-) 1206 WP_008791318.1 ATP-grasp domain-containing protein -
  LA327_RS13410 (LA327_13410) - 2714720..2715457 (-) 738 WP_008791317.1 esterase family protein -
  LA327_RS13415 (LA327_13415) - 2715457..2716647 (-) 1191 WP_003536128.1 ATP-grasp domain-containing protein -
  LA327_RS13420 (LA327_13420) - 2716787..2717644 (-) 858 WP_008791316.1 type II secretion system protein -
  LA327_RS13425 (LA327_13425) pilT 2717665..2718708 (-) 1044 WP_008791315.1 type IV pilus twitching motility protein PilT Machinery gene
  LA327_RS13430 (LA327_13430) - 2718718..2720388 (-) 1671 WP_003536125.1 GspE/PulE family protein -
  LA327_RS13435 (LA327_13435) - 2720406..2720795 (-) 390 WP_008791314.1 hypothetical protein -
  LA327_RS13440 (LA327_13440) - 2720883..2721830 (-) 948 WP_008791313.1 type II secretion system protein -
  LA327_RS13445 (LA327_13445) - 2721868..2722800 (-) 933 WP_003536123.1 type II secretion system protein -
  LA327_RS13450 (LA327_13450) - 2722797..2723261 (-) 465 WP_008791312.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38957.86 Da        Isoelectric Point: 6.2847

>NTDB_id=606721 LA327_RS13425 WP_008791315.1 2717665..2718708(-) (pilT) [Thomasclavelia ramosa strain FDAARGOS_1519]
MINEILKASRNNKCSDIHISANTGIKVRQDGLLADYPIEFPQNELIKMIMELIPKRLIDNVDRHEDADFVYHVGEERYRV
NIYYEQTNICAAIRVINDEILTLEQLEMPTVLNQIAMEPRGLVLVTGPTGSGKSTTLAAMIDLVNKQRNCHILTIEDPIE
YVYKQKQALIHQREIGEDVKDFDMALRSAMREDPDVILVGEMRDYETISAVITLAETGHLVFSTLHTIGAAKTIDRIIDI
FPQHKQAQVRTQLSGVLNAVITQTLLPHASGIGRVAAVEIMRANDAVRNLIRDNKGHQINSVIQTGKKEGMISLNHALAN
LVREGKITLDTAKKCASDISEFKQYLQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=606721 LA327_RS13425 WP_008791315.1 2717665..2718708(-) (pilT) [Thomasclavelia ramosa strain FDAARGOS_1519]
ATGATAAATGAAATTTTAAAAGCTTCTCGAAATAATAAATGTTCAGATATTCATATTAGTGCTAATACAGGAATAAAGGT
TCGTCAGGATGGTTTATTGGCAGATTATCCAATTGAGTTTCCTCAAAATGAATTAATCAAAATGATTATGGAGTTAATTC
CAAAACGATTGATTGATAATGTTGATCGTCATGAAGATGCAGATTTTGTCTATCATGTTGGGGAGGAACGTTATCGGGTC
AATATTTATTATGAACAAACTAATATTTGTGCTGCAATTAGGGTTATTAATGATGAAATTTTAACATTAGAGCAATTAGA
AATGCCCACCGTGTTAAATCAGATTGCAATGGAACCAAGAGGCTTAGTTTTAGTAACAGGACCGACAGGAAGTGGTAAAT
CAACTACTCTAGCAGCAATGATTGATTTAGTGAATAAACAGAGAAATTGTCATATTTTAACAATTGAAGATCCAATTGAA
TATGTTTATAAACAGAAACAAGCATTAATTCATCAACGTGAAATTGGTGAAGATGTAAAAGATTTTGATATGGCATTACG
CTCTGCTATGCGTGAAGACCCGGATGTTATTTTAGTTGGTGAAATGCGTGATTACGAAACAATCAGTGCCGTTATTACAT
TAGCCGAAACTGGTCATTTAGTTTTCTCAACGTTGCACACGATTGGGGCAGCTAAGACAATTGATCGAATAATTGATATT
TTTCCTCAGCATAAACAGGCTCAAGTTAGAACTCAATTATCAGGTGTTTTAAATGCGGTAATTACACAAACTTTATTACC
TCATGCAAGCGGAATAGGACGAGTTGCTGCGGTAGAAATAATGCGTGCTAATGATGCAGTTAGAAATTTAATTCGTGATA
ATAAAGGCCACCAGATCAATTCTGTTATTCAAACGGGTAAAAAAGAAGGGATGATATCTTTAAATCATGCTCTTGCAAAT
CTAGTGCGTGAAGGAAAAATAACTTTAGATACAGCTAAAAAGTGTGCTAGTGATATTTCAGAGTTTAAACAGTATTTACA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

48.986

99.424

0.487
  pilT Legionella pneumophila strain ERS1305867

48.116

99.424

0.478
  pilT Legionella pneumophila strain Lp02

48.116

99.424

0.478
  pilT Neisseria meningitidis 8013

48.529

97.983

0.476
  pilT Acinetobacter nosocomialis M2

47.536

99.424

0.473
  pilT Acinetobacter baumannii D1279779

47.536

99.424

0.473
  pilT Acinetobacter baumannii strain A118

47.536

99.424

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.536

99.424

0.473
  pilT Neisseria gonorrhoeae MS11

48.235

97.983

0.473
  pilT Pseudomonas aeruginosa PAK

47.788

97.695

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

94.524

0.464
  pilT Vibrio cholerae strain A1552

49.085

94.524

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

100

0.464