Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K4O48_RS18365 Genome accession   NZ_CP082271
Coordinates   3963994..3965139 (-) Length   381 a.a.
NCBI ID   WP_045164002.1    Uniprot ID   A0A0D9AEN8
Organism   Pseudomonas sp. DNDY-54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3958994..3970139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4O48_RS18340 - 3959061..3960077 (+) 1017 WP_222909774.1 aspartate carbamoyltransferase catalytic subunit -
  K4O48_RS18345 - 3960077..3961348 (+) 1272 WP_222909775.1 dihydroorotase -
  K4O48_RS18350 - 3961400..3961798 (-) 399 WP_222909776.1 NINE protein -
  K4O48_RS18355 - 3961937..3963154 (+) 1218 WP_222909777.1 putative DNA modification/repair radical SAM protein -
  K4O48_RS18360 - 3963154..3963993 (+) 840 WP_222909778.1 TIGR03915 family putative DNA repair protein -
  K4O48_RS18365 pilU 3963994..3965139 (-) 1146 WP_045164002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K4O48_RS18370 pilT 3965218..3966252 (-) 1035 WP_222909779.1 type IV pilus twitching motility protein PilT Machinery gene
  K4O48_RS18375 - 3966319..3967032 (+) 714 WP_222909780.1 YggS family pyridoxal phosphate-dependent enzyme -
  K4O48_RS18380 proC 3967045..3967872 (+) 828 WP_222909781.1 pyrroline-5-carboxylate reductase -
  K4O48_RS18385 - 3968150..3968740 (+) 591 WP_222909782.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42430.68 Da        Isoelectric Point: 6.5054

>NTDB_id=600706 K4O48_RS18365 WP_045164002.1 3963994..3965139(-) (pilU) [Pseudomonas sp. DNDY-54]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKILPVNKTPMSPEMTRETVHAVMNEQQRREFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTDLGMQTFDQA
LYNLYVQGEITYEDALLHADSSNDLRLMIKLGSETDGEHLTSVSQGLTLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=600706 K4O48_RS18365 WP_045164002.1 3963994..3965139(-) (pilU) [Pseudomonas sp. DNDY-54]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATTACCGCGGGTGTGCCGCCTTC
GATGAAGGTTAACGGCAAAATCCTCCCGGTGAACAAGACGCCAATGTCGCCGGAGATGACTCGTGAGACCGTGCATGCGG
TGATGAACGAGCAACAGCGCCGTGAGTTCGCTGAAAATCACGAATGTAACTTCGCCATTAGCGCACGCGGTATAGGCCGC
TTTCGTGTCAGCGCCTTCTACCAGCGCAATCTTGCCGGCATGGTACTGCGCCGGATCGAAACCAACATACCAACGCTCGA
AGACCTGAAACTGCCCGATGTTCTGAAAAAGCTGGCGATGACCAAGCGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCCACCTCGCTGGCGGCAATGATCGGTTATCGCAACAAGAACTCCAGCGGCCATATCATTTCCATCGAAGAC
CCGATCGAGTTCATCCATCAGCATCAGAGCTGCATCGTGACCCAGCGTGAGGTCGGCATCGACACCGATTCCTTCGATGT
CGCCCTGAAAAACACGCTTCGTCAGGCACCCGACGTGATTCTCATCGGCGAAGTTCGGACTCGTGAAACCATGGATTACG
CGGTCGCGTTCGCTGAAACCGGCCATCTTTGCCTGGCAACTTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCCGACCGACAGCAGCAGGTGTGGATGGACTTGTCGCTCAACCTCAAGGCGATTGTCGCTCAGCA
GCTGATTCCGACGCCCGACGGTAAAGGCCGTCGCGCCGTCATCGAGGTGCTGATCAACACGCCGCTCGCGGCAGACCTTA
TTCGCAAGGGCGAAGTGCACGAGCTGAAGTCGCTGATGAAGCGCTCGACTGATCTGGGTATGCAGACCTTCGACCAGGCG
CTTTACAACCTCTATGTGCAAGGCGAAATCACTTACGAAGACGCGCTACTACATGCCGATTCGTCCAACGACCTGCGACT
GATGATCAAGTTGGGGTCGGAAACCGATGGCGAGCACCTGACGAGCGTCTCCCAGGGTCTGACGCTGGAAGTCAGCGACG
ATGATCCGGGCCGCAGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D9AEN8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

97.113

100

0.971
  pilU Acinetobacter baylyi ADP1

66.106

93.701

0.619
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Neisseria meningitidis 8013

40.87

90.551

0.37
  pilT Legionella pneumophila strain Lp02

42.09

87.927

0.37
  pilT Legionella pneumophila strain ERS1305867

42.09

87.927

0.37
  pilT Neisseria gonorrhoeae MS11

40.87

90.551

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.42

88.714

0.367
  pilT Vibrio cholerae strain A1552

41.42

88.714

0.367
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365
  pilT Pseudomonas stutzeri DSM 10701

41.194

87.927

0.362