Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6982_RS02315 Genome accession   NZ_CP082215
Coordinates   559843..560976 (-) Length   377 a.a.
NCBI ID   WP_104602238.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain MI_359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 554843..565976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6982_RS02290 (K6982_02300) - 555089..555424 (-) 336 WP_104602233.1 phasin family protein -
  K6982_RS02295 (K6982_02305) queD 555617..555970 (-) 354 WP_104602234.1 6-carboxytetrahydropterin synthase QueD -
  K6982_RS02300 (K6982_02310) - 556121..557509 (-) 1389 WP_104602235.1 DEAD/DEAH box helicase -
  K6982_RS02305 (K6982_02315) - 557887..558876 (+) 990 WP_104602236.1 fumarylacetoacetate hydrolase family protein -
  K6982_RS02310 (K6982_02320) maiA 558911..559573 (+) 663 WP_104602237.1 maleylacetoacetate isomerase -
  K6982_RS02315 (K6982_02325) pilU 559843..560976 (-) 1134 WP_104602238.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6982_RS02320 (K6982_02330) - 561075..561458 (+) 384 WP_159406557.1 DUF4398 domain-containing protein -
  K6982_RS02325 (K6982_02335) - 561455..562345 (+) 891 WP_274392040.1 hypothetical protein -
  K6982_RS02330 (K6982_02340) - 562688..562906 (+) 219 WP_104602241.1 YdcH family protein -
  K6982_RS02335 (K6982_02345) - 563145..564515 (+) 1371 WP_159406560.1 pyridoxal-phosphate dependent enzyme -
  K6982_RS02340 (K6982_02350) - 564605..565804 (+) 1200 WP_159406561.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41633.94 Da        Isoelectric Point: 6.2909

>NTDB_id=600240 K6982_RS02315 WP_104602238.1 559843..560976(-) (pilU) [Xanthomonas cucurbitae strain MI_359]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIAELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKLSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGTDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=600240 K6982_RS02315 WP_104602238.1 559843..560976(-) (pilU) [Xanthomonas cucurbitae strain MI_359]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAGAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACCGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGATGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAACGCGGCGAGGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGC
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGGT
CGGGCAAGTCCACCTCGCTGGCTTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCTATCGAATACCTGCACAAGCACAAGCTGTCGATCGTGAATCAGCGCGAGGTGGGGCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACCATGGAGGCGG
CCATTGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCGCAGCG
CCTGGTCAAGGGCACGGATGGACGTCGCCGGCCGGCGACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GCCGCGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGTCGGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGATTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAACACGACCCCTATGCCGACTACGAGGCGGCATCGGCCCCGAAGATCAGTC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366