NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	K6981_RS14945	Genome accession	NZ_CP082213
Coordinates	3386969..3387388 (-)	Length	139 a.a.
NCBI ID	WP_274396444.1	Uniprot ID	-
Organism	Xanthomonas cucurbitae strain WI_206
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3378575..3389000	3386969..3387388	within	0

Gene organization within MGE regions

Location: 3378575..3389000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6981_RS14915 (K6981_14860)	-	3378575..3378793 (+)	219	WP_274396441.1	hypothetical protein	-
K6981_RS14920 (K6981_14865)	sucD	3378911..3379786 (-)	876	WP_104604276.1	succinate--CoA ligase subunit alpha	-
K6981_RS14925 (K6981_14870)	sucC	3379811..3380980 (-)	1170	WP_274396442.1	ADP-forming succinate--CoA ligase subunit beta	-
K6981_RS14930 (K6981_14875)	-	3381216..3382829 (+)	1614	WP_104604280.1	HAMP domain-containing sensor histidine kinase	-
K6981_RS14935 (K6981_14880)	pilR	3382989..3384380 (+)	1392	WP_170066996.1	sigma-54 dependent transcriptional regulator	Regulator
K6981_RS14940 (K6981_14885)	pilB	3385017..3386726 (-)	1710	WP_274397236.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K6981_RS14945 (K6981_14890)	pilA2	3386969..3387388 (-)	420	WP_274396444.1	pilin	Machinery gene
K6981_RS14950 (K6981_14895)	pilC	3387741..3389000 (+)	1260	WP_274396445.1	type II secretion system F family protein	Machinery gene

Sequence

Protein

Download Length: 139 a.a. Molecular weight: 13940.07 Da Isoelectric Point: 9.0253

                                >NTDB_id=600219 K6981_RS14945 WP_274396444.1 3386969..3387388(-) (pilA2) [Xanthomonas cucurbitae strain WI_206]

MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLIKARVSEVVLAASSARTTVSEAAAADTGTAMPAAVTIESQASKYVA

SVAYAGGTTNGVITATSQALGGTATGTITLTGTKSANGQVAWVCGGTIPAKYRPATCQG

Nucleotide

Download Length: 420 bp

                                >NTDB_id=600219 K6981_RS14945 WP_274396444.1 3386969..3387388(-) (pilA2) [Xanthomonas cucurbitae strain WI_206]

ATGAAGAAACAACAAGGTTTTACCCTGATCGAATTGATGATCGTGGTTGCGATCATCGCTATTCTGGCCGCTATCGCGCT

GCCAGCCTACCAGGACTACCTGATCAAAGCGCGGGTGTCGGAAGTGGTGCTCGCAGCTTCGTCGGCTCGTACTACCGTTT

CTGAAGCCGCAGCTGCTGACACCGGCACGGCTATGCCTGCAGCAGTGACGATTGAGAGCCAGGCATCCAAGTACGTTGCC

TCTGTGGCTTATGCTGGCGGTACAACTAATGGCGTTATCACTGCGACATCCCAGGCTCTGGGTGGTACCGCCACCGGCAC

GATTACGTTGACGGGCACCAAGTCTGCTAATGGACAGGTCGCTTGGGTCTGTGGCGGCACGATTCCTGCAAAGTACCGCC

CGGCAACTTGCCAAGGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-138)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	56.028	100	0.568
pilA2	Legionella pneumophila strain ERS1305867	55.319	100	0.561
comP	Acinetobacter baylyi ADP1	48.026	100	0.525
pilA	Ralstonia pseudosolanacearum GMI1000	41.718	100	0.489
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	35.638	100	0.482
pilA/pilA1	Eikenella corrodens VA1	40.132	100	0.439
pilE	Neisseria gonorrhoeae MS11	37.5	100	0.432
pilE	Neisseria gonorrhoeae strain FA1090	36.42	100	0.424
pilA	Haemophilus influenzae 86-028NP	39.716	100	0.403
pilA	Vibrio parahaemolyticus RIMD 2210633	39.855	99.281	0.396
pilA	Acinetobacter baumannii strain A118	37.143	100	0.374

Detailed information NTG0600219