Detailed information NTG0598652

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K5K95_RS24125
• Genome accession: NZ_CP081946
• Coordinates: 5324365..5325474 (+)
• Length: 369 a.a.
• NCBI ID: WP_222586444.1
• Uniprot ID: -
• Organism: Pseudomonas sp. DR48
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5319365..5330474

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5K95_RS24110 (K5K95_24110)	-	5320288..5321220 (+)	933	WP_222586442.1	sugar kinase	-
K5K95_RS24115 (K5K95_24115)	-	5321364..5321639 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
K5K95_RS24120 (K5K95_24120)	-	5321706..5324243 (-)	2538	WP_222586443.1	PAS domain-containing protein	-
K5K95_RS24125 (K5K95_24125)	pilU	5324365..5325474 (+)	1110	WP_222586444.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K5K95_RS24130 (K5K95_24130)	-	5325494..5327449 (-)	1956	WP_222586445.1	acetoacetate--CoA ligase	-
K5K95_RS24135 (K5K95_24135)	hbdH	5327605..5328378 (-)	774	WP_222586446.1	3-hydroxybutyrate dehydrogenase	-
K5K95_RS24140 (K5K95_24140)	-	5328386..5329777 (-)	1392	WP_222586447.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40990.95 Da
• Isoelectric Point: 6.3926

>NTDB_id=598652 K5K95_RS24125 WP_222586444.1 5324365..5325474(+) (pilU) [Pseudomonas sp. DR48]
MEIDVLLRILAHQDGSDLYLSTGAPPSARFDGVLKPLADQPFKPGEVAAIAESIMDAEQRLEFDRELEMNLAISLAGIGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLMLFVGATASGKSTSLAALIDYRNRHSSGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIADLIRSNEFADLKGIMEKSVELGMQTFDAAL
YGLVVEGAISEEEALKHADSVNNLRLRLKLHADAAPSTQPASGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=598652 K5K95_RS24125 WP_222586444.1 5324365..5325474(+) (pilU) [Pseudomonas sp. DR48]
ATGGAAATCGATGTGTTGTTACGGATACTGGCCCATCAGGACGGATCCGATCTTTACCTGTCCACCGGTGCGCCTCCCAG
CGCGCGGTTCGACGGCGTGCTCAAGCCGCTGGCCGATCAACCGTTCAAACCGGGGGAGGTCGCGGCCATTGCCGAGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGTGAACTAGAAATGAACCTGGCGATTTCGCTGGCGGGCATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGATGTGTCAATCGTGGCGCGCAATATCAAGCTCGACATTCCACGCTTCGA
GGACCTCAAGCTGCCGGCGGTGCTGCTCGAAACCGTGATGCTCAAGCAAGGCCTGATGCTGTTCGTCGGGGCTACGGCCT
CGGGCAAGTCTACGTCGCTGGCGGCGCTGATCGATTACCGCAACCGTCACAGCAGTGGCCATATCGTCACCATCGAAGAC
CCGATCGAATACATCCATCGCCACAAGCGTTCGATCATCAACCAGCGTGAAGTCGGGGTCGATACCCGCAGTTTTCATGC
TGCCTTGAAAAATACCCTGCGCCAGGCCCCGGATGTGGTGCTGATCGGCGAAATCCGTGACCGGGAAACAATGGAGCACG
CACTGGCGTTCGCCGACACTGGCCATCTGGTGATTTCCACCTTGCATGCGCACAACGCCCATCAGGCGCTGGACCGCATT
ATCAATTTTTTCCCCGAAGAGCGCCGCGCGCAACTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAACG
GCTGGTGCGCACCCGCGACGGTCAGCGTCGGGCAGCGGTGGAAGTGATGCTGGGCACACCAACCATTGCCGACCTGATTC
GTAGCAACGAGTTTGCCGATCTCAAGGGGATCATGGAGAAGTCGGTGGAACTGGGCATGCAGACGTTTGACGCCGCATTG
TATGGGTTGGTGGTCGAAGGGGCGATCAGCGAGGAAGAAGCGCTCAAGCATGCGGATTCGGTGAACAACCTTCGCCTGCG
GCTGAAGTTGCATGCCGACGCGGCTCCATCCACTCAGCCAGCCTCCGGGGAATGGGGCCTCATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.183	96.206	0.55
pilU	Acinetobacter baylyi ADP1	52.528	96.477	0.507
pilU	Vibrio cholerae strain A1552	52	94.851	0.493
pilT	Pseudomonas aeruginosa PAK	41.57	93.225	0.388
pilT	Pseudomonas stutzeri DSM 10701	40.988	93.225	0.382
pilT	Legionella pneumophila strain Lp02	41.317	90.515	0.374
pilT	Legionella pneumophila strain ERS1305867	41.317	90.515	0.374
pilT	Vibrio cholerae strain A1552	41.141	90.244	0.371
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	90.244	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.118	91.87	0.369
pilT	Acinetobacter baumannii D1279779	39.82	90.515	0.36
pilT	Acinetobacter baumannii strain A118	39.82	90.515	0.36
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36