Detailed information NTG0595491

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K3369_RS16925
• Genome accession: NZ_CP081178
• Coordinates: 3768619..3769728 (-)
• Length: 369 a.a.
• NCBI ID: WP_102595012.1
• Uniprot ID: -
• Organism: Pseudomonas mandelii strain KGI_MA19
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3763619..3774728

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K3369_RS16920 (K3369_16920)	-	3764633..3768493 (-)	3861	WP_221106131.1	hypothetical protein	-
K3369_RS16925 (K3369_16925)	pilU	3768619..3769728 (-)	1110	WP_102595012.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K3369_RS16930 (K3369_16930)	-	3769850..3772387 (+)	2538	WP_221106132.1	PAS domain-containing protein	-
K3369_RS16935 (K3369_16935)	-	3772453..3772728 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
K3369_RS16940 (K3369_16940)	-	3772850..3773785 (-)	936	WP_083376588.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41026.93 Da
• Isoelectric Point: 6.0924

>NTDB_id=595491 K3369_RS16925 WP_102595012.1 3768619..3769728(-) (pilU) [Pseudomonas mandelii strain KGI_MA19]
MEIDALLQILSDRNGSDLYLSTGAPPSARFDGVLKPLTDQPFKPGAVAAIAESIMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDCGKSTSLAALIDYRNRHSTGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLSNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIGDLIRRNQLDELKDIMEKSAEFGMQTFDGAL
YALVMEGAISEEEALKHADSLSNLKLRLKLYGGGPSTVNAPNGDWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=595491 K3369_RS16925 WP_102595012.1 3768619..3769728(-) (pilU) [Pseudomonas mandelii strain KGI_MA19]
ATGGAAATCGATGCACTGTTGCAGATACTGTCTGACCGGAATGGATCCGATCTCTATCTTTCCACCGGCGCACCACCCAG
TGCGCGGTTCGACGGTGTGCTCAAGCCGCTGACCGACCAGCCATTCAAGCCGGGAGCAGTCGCGGCCATTGCCGAGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCAATCTCCCTGGCGGGTGTCGGGCGC
TTTCGGGTCAACATCTTCAAGCAACGCAACGATGTGTCAATCGTGGCACGCAACATCAAGCTCGACATCCCGCGTTTCGA
AGACCTCAAACTACCGGCGGTGCTGCTCGAAACCGTGATGCTCAAACAAGGATTGATACTGTTCGTCGGCGCCACCGATT
GCGGCAAGTCAACCTCCCTCGCGGCGCTGATCGATTACCGCAACCGCCACAGCACTGGCCATATCGTCACCATCGAGGAC
CCGATCGAGTACATCCATCGGCACAAGCAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTTCATGC
TGCCCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTACTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGATACGGGCCATCTGGTGATCTCGACGTTGCACGCGCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAATTTCTTTCCCGAAGAGCGGCGGACGCAGTTGCTGCATGACTTGAGCAACAATCTGAAAGCGTTTGTTTCCCAACG
ATTGGTGCGGACCCTTGACGGTCAGCGCAGGGCGGCAGTGGAAGTGATGTTGGGGACACCCACGATCGGTGACTTGATAA
GGCGCAATCAACTGGACGAACTTAAAGACATCATGGAAAAGTCCGCAGAGTTCGGTATGCAGACGTTCGATGGCGCACTG
TATGCACTGGTGATGGAAGGCGCAATCAGTGAGGAAGAGGCGCTAAAACATGCGGACTCGCTGAGCAATCTGAAGTTGCG
CCTCAAGCTGTATGGCGGTGGGCCTTCGACCGTCAATGCGCCCAATGGGGACTGGGGGTTAGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.556	95.122	0.528
pilU	Acinetobacter baylyi ADP1	51.989	95.393	0.496
pilU	Vibrio cholerae strain A1552	50.833	97.561	0.496
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.741	95.122	0.388
pilT	Vibrio cholerae strain A1552	40.741	95.122	0.388
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Pseudomonas stutzeri DSM 10701	39.826	93.225	0.371
pilT	Legionella pneumophila strain Lp02	41.018	90.515	0.371
pilT	Legionella pneumophila strain ERS1305867	41.018	90.515	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36
pilT	Acinetobacter baumannii D1279779	39.82	90.515	0.36
pilT	Acinetobacter baumannii strain A118	39.82	90.515	0.36
pilT	Acinetobacter nosocomialis M2	39.82	90.515	0.36