Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   KX728_RS00810 Genome accession   NZ_CP079724
Coordinates   156576..157517 (+) Length   313 a.a.
NCBI ID   WP_215805168.1    Uniprot ID   -
Organism   Streptococcus oralis strain 34     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151576..162517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KX728_RS00790 (KX728_00790) tgt 151610..152752 (+) 1143 WP_001285249.1 tRNA guanosine(34) transglycosylase Tgt -
  KX728_RS00795 (KX728_00795) - 152886..154703 (+) 1818 WP_215805170.1 acyltransferase family protein -
  KX728_RS00800 (KX728_00800) nagA 154844..155995 (+) 1152 WP_215805169.1 N-acetylglucosamine-6-phosphate deacetylase -
  KX728_RS00805 (KX728_00805) - 156147..156512 (+) 366 WP_000286399.1 DUF1033 family protein -
  KX728_RS00810 (KX728_00810) comGA/cglA/cilD 156576..157517 (+) 942 WP_215805168.1 competence type IV pilus ATPase ComGA Machinery gene
  KX728_RS00815 (KX728_00815) comGB/cglB 157465..158481 (+) 1017 WP_215805178.1 competence type IV pilus assembly protein ComGB Machinery gene
  KX728_RS00820 (KX728_00820) comGC/cglC 158483..158806 (+) 324 WP_215805167.1 competence type IV pilus major pilin ComGC Machinery gene
  KX728_RS00825 (KX728_00825) comGD/cglD 158769..159203 (+) 435 WP_215805166.1 competence type IV pilus minor pilin ComGD Machinery gene
  KX728_RS00830 (KX728_00830) comGE/cglE 159166..159468 (+) 303 WP_000413364.1 competence type IV pilus minor pilin ComGE Machinery gene
  KX728_RS00835 (KX728_00835) comGF/cglF 159431..159892 (+) 462 WP_215805165.1 competence type IV pilus minor pilin ComGF Machinery gene
  KX728_RS00840 (KX728_00840) comGG/cglG 159870..160283 (+) 414 WP_215805164.1 competence type IV pilus minor pilin ComGG Machinery gene
  KX728_RS00845 (KX728_00845) - 160316..160906 (+) 591 Protein_149 class I SAM-dependent methyltransferase -
  KX728_RS00850 (KX728_00850) comYH 160965..161918 (+) 954 WP_215805163.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35485.42 Da        Isoelectric Point: 5.5992

>NTDB_id=588947 KX728_RS00810 WP_215805168.1 156576..157517(+) (comGA/cglA/cilD) [Streptococcus oralis strain 34]
MVQEIAQKIIATAKEKKAQDIYFIPKEKSYELHMRVGDERCLVDSYEFDVLAAVISHFKFVAGMNVGEKRRSQLGSCDYQ
HGEKVSSLRLSTVGDYRGHESLVIRLLHDEEQELHFWFQDMNELGEQYRQRGLYLFAGPVGSGKTTLMHELAKPLFKGQQ
VMSIEDPVEIKQEDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVTEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQPTSWNAQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=588947 KX728_RS00810 WP_215805168.1 156576..157517(+) (comGA/cglA/cilD) [Streptococcus oralis strain 34]
ATGGTACAAGAAATTGCACAAAAAATTATTGCTACTGCGAAAGAAAAGAAGGCCCAGGATATCTATTTTATCCCCAAGGA
AAAGTCCTACGAACTTCACATGCGGGTTGGAGACGAACGGTGTCTCGTTGACTCCTATGAGTTTGATGTTTTAGCTGCAG
TGATTAGTCATTTTAAGTTTGTGGCGGGTATGAACGTAGGAGAGAAGAGACGTAGTCAGCTGGGCTCTTGCGACTATCAG
CATGGGGAGAAGGTGTCTTCTCTGCGTTTGTCTACCGTAGGAGATTATCGGGGACATGAGAGTTTGGTCATTCGTTTGTT
GCACGATGAGGAGCAGGAGCTGCATTTCTGGTTTCAGGATATGAACGAACTGGGTGAGCAGTACAGGCAACGGGGACTCT
ATCTCTTTGCAGGTCCAGTCGGCAGTGGCAAGACGACTCTGATGCACGAATTAGCTAAGCCTCTTTTTAAGGGGCAGCAG
GTCATGTCCATTGAAGATCCTGTTGAGATTAAGCAGGAAGACATGCTCCAGTTGCAGTTAAATGAGGCGATTGGCTTGAC
CTATGAAAATCTGATCAAACTGTCTCTCCGGCATCGTCCTGATCTCTTGATTATTGGCGAAATTCGGGATAGCGAGACAG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTTTTTTCAACCATTCATGCCAAGAGTATCCGAGGAGTTTAT
GAACGCCTTCTGGAGTTGGGTGTGACGGAGGAGGAATTAGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGCCAACTAGCTGGAATGCGCAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

89.457

100

0.895
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae D39

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae R6

87.859

100

0.879
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

87.859

100

0.879
  comYA Streptococcus gordonii str. Challis substr. CH1

78.71

99.042

0.78
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.645

99.042

0.601
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

44.061

83.387

0.367