NTDB

Overview

Name	comGB/cglB	Type	Machinery gene
Locus tag	KX728_RS00815	Genome accession	NZ_CP079724
Coordinates	157465..158481 (+)	Length	338 a.a.
NCBI ID	WP_215805178.1	Uniprot ID	-
Organism	Streptococcus oralis strain 34
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 152465..163481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KX728_RS00795 (KX728_00795)	-	152886..154703 (+)	1818	WP_215805170.1	acyltransferase family protein	-
KX728_RS00800 (KX728_00800)	nagA	154844..155995 (+)	1152	WP_215805169.1	N-acetylglucosamine-6-phosphate deacetylase	-
KX728_RS00805 (KX728_00805)	-	156147..156512 (+)	366	WP_000286399.1	DUF1033 family protein	-
KX728_RS00810 (KX728_00810)	comGA/cglA/cilD	156576..157517 (+)	942	WP_215805168.1	competence type IV pilus ATPase ComGA	Machinery gene
KX728_RS00815 (KX728_00815)	comGB/cglB	157465..158481 (+)	1017	WP_215805178.1	competence type IV pilus assembly protein ComGB	Machinery gene
KX728_RS00820 (KX728_00820)	comGC/cglC	158483..158806 (+)	324	WP_215805167.1	competence type IV pilus major pilin ComGC	Machinery gene
KX728_RS00825 (KX728_00825)	comGD/cglD	158769..159203 (+)	435	WP_215805166.1	competence type IV pilus minor pilin ComGD	Machinery gene
KX728_RS00830 (KX728_00830)	comGE/cglE	159166..159468 (+)	303	WP_000413364.1	competence type IV pilus minor pilin ComGE	Machinery gene
KX728_RS00835 (KX728_00835)	comGF/cglF	159431..159892 (+)	462	WP_215805165.1	competence type IV pilus minor pilin ComGF	Machinery gene
KX728_RS00840 (KX728_00840)	comGG/cglG	159870..160283 (+)	414	WP_215805164.1	competence type IV pilus minor pilin ComGG	Machinery gene
KX728_RS00845 (KX728_00845)	-	160316..160906 (+)	591	Protein_149	class I SAM-dependent methyltransferase	-
KX728_RS00850 (KX728_00850)	comYH	160965..161918 (+)	954	WP_215805163.1	class I SAM-dependent methyltransferase	Machinery gene
KX728_RS00855 (KX728_00855)	-	161969..163159 (+)	1191	WP_215805162.1	acetate kinase	-

Sequence

Protein

Download Length: 338 a.a. Molecular weight: 38522.57 Da Isoelectric Point: 9.5636

                                >NTDB_id=588948 KX728_RS00815 WP_215805178.1 157465..158481(+) (comGB/cglB) [Streptococcus oralis strain 34]

MDISQVFRLKRKKLATAKQKKIITLFNNLFSSGFHLVEIISFLGRSALLEKDYVAQMHQGLSQGKSFSEMMNSLGFSSAI

VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNFATQIIGNMPQIF

LGLVLVCSLSLLLALTFYKRSSKMQVFSMLARVPFLGIFVQTYLTAYYGREWGNMISQGMELTQIFQIMQEQGSQLFKEI

GQDLAQALQNGREFSQTIATYHFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVTRTMNLVQPLVFIFVALIIV

LLYAAMLMPMYQNMEVNF

Nucleotide

Download Length: 1017 bp

                                >NTDB_id=588948 KX728_RS00815 WP_215805178.1 157465..158481(+) (comGB/cglB) [Streptococcus oralis strain 34]

ATGGACATATCACAAGTCTTCAGGCTGAAACGGAAAAAATTAGCTACAGCTAAGCAGAAGAAAATCATCACTTTGTTTAA

CAACCTCTTCTCCAGTGGCTTTCATTTGGTGGAAATTATTTCTTTCTTGGGAAGAAGTGCCCTGCTGGAAAAGGACTATG

TGGCCCAGATGCACCAAGGTTTGTCGCAGGGGAAATCCTTTTCAGAAATGATGAACAGCTTGGGTTTTTCAAGTGCCATC

GTGACCCAATTATCTCTAGCTGAAGTGCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGATAATTT

GGCCAAGGTCAAGAAAAAGTTAATCGAAGTGGCGACCTATCCCCTGATTTTGCTGGGATTTCTCTTGCTAATTATGTTGG

GGCTCAGGAATTATTTGCTCCCCCAACTGGACAGTAGCAATTTCGCCACTCAAATCATCGGCAATATGCCACAAATATTT

CTGGGACTAGTGCTGGTTTGCTCTCTATCTTTACTTTTAGCCCTCACTTTCTACAAAAGAAGTTCCAAGATGCAGGTTTT

CTCTATGTTGGCACGGGTTCCCTTTCTAGGAATCTTTGTCCAGACTTATCTGACAGCTTATTATGGGCGTGAATGGGGCA

ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAGATCATGCAGGAACAGGGCTCCCAGCTCTTTAAAGAAATC

GGTCAAGATCTGGCTCAAGCCCTGCAAAATGGCCGCGAATTTTCTCAAACCATAGCGACCTATCATTTCTTTAAAAAGGA

GTTGAGTCTTATCATCGAGTATGGGGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG

AAGCCTTTTTTACCCGAGTTACTCGTACCATGAACTTAGTACAGCCACTGGTTTTTATCTTTGTGGCTCTGATTATCGTT

TTACTTTATGCGGCAATGCTTATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(210-329)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGB/cglB	Streptococcus mitis SK321	92.012	100	0.92
comGB/cglB	Streptococcus mitis NCTC 12261	91.124	100	0.911
comGB/cglB	Streptococcus pneumoniae Rx1	89.941	100	0.899
comGB/cglB	Streptococcus pneumoniae D39	89.941	100	0.899
comGB/cglB	Streptococcus pneumoniae R6	89.941	100	0.899
comGB/cglB	Streptococcus pneumoniae TIGR4	89.941	100	0.899
comYB	Streptococcus gordonii str. Challis substr. CH1	72.917	99.408	0.725
comYB	Streptococcus mutans UA140	58.934	94.379	0.556
comYB	Streptococcus mutans UA159	58.934	94.379	0.556
comGB	Lactococcus lactis subsp. cremoris KW2	52.367	100	0.524

Detailed information NTG0588948