Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L40_RS15705 Genome accession   NZ_CP077401
Coordinates   3383560..3384699 (+) Length   379 a.a.
NCBI ID   WP_071911119.1    Uniprot ID   A0AAQ0E809
Organism   Aeromonas sp. FDAARGOS 1410     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3378560..3389699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L40_RS15680 (I6L40_15680) - 3378923..3379987 (+) 1065 WP_041208564.1 3-deoxy-7-phosphoheptulonate synthase -
  I6L40_RS15685 (I6L40_15685) - 3380415..3381200 (-) 786 WP_216994539.1 ATP-binding cassette domain-containing protein -
  I6L40_RS15690 (I6L40_15690) btuC 3381187..3382194 (-) 1008 WP_201929531.1 vitamin B12 ABC transporter permease BtuC -
  I6L40_RS15695 (I6L40_15695) - 3382297..3383031 (-) 735 WP_216994540.1 ABC transporter substrate-binding protein -
  I6L40_RS15700 (I6L40_15700) - 3383144..3383500 (+) 357 WP_005302630.1 DMT family protein -
  I6L40_RS15705 (I6L40_15705) pilU 3383560..3384699 (+) 1140 WP_071911119.1 type IVa pilus ATPase TapW Machinery gene
  I6L40_RS15710 (I6L40_15710) - 3384766..3385215 (-) 450 WP_071911117.1 isoprenylcysteine carboxylmethyltransferase family protein -
  I6L40_RS15715 (I6L40_15715) alr 3385391..3386617 (+) 1227 WP_201929528.1 alanine racemase -
  I6L40_RS15720 (I6L40_15720) - 3386674..3387618 (-) 945 WP_041208571.1 TerC family protein -
  I6L40_RS15725 (I6L40_15725) oppF 3387921..3388916 (-) 996 WP_216994541.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42259.30 Da        Isoelectric Point: 6.6720

>NTDB_id=581260 I6L40_RS15705 WP_071911119.1 3383560..3384699(+) (pilU) [Aeromonas sp. FDAARGOS 1410]
MELRDMLQTLAKQDGSDLYLSTGAPPCAKFNGVLRPLNETPLNPGDVAAIAEAIMDKEQKEQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDDKGDHF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=581260 I6L40_RS15705 WP_071911119.1 3383560..3384699(+) (pilU) [Aeromonas sp. FDAARGOS 1410]
ATGGAATTGCGAGACATGTTACAGACCCTGGCCAAACAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAATTCAACGGGGTACTGCGCCCCCTGAATGAGACACCGCTCAACCCGGGCGATGTGGCGGCCATTGCCGAGGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCGCAGATCGGTCGT
TTCCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTAGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
AGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGACTGGTGCTATTCGTTGGCGGTACCGGCT
CGGGTAAATCGACCTCGCTGGCCGCCCTCATCGACCATCGCAACCGCAATAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAAGTTGGCGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATCCTTATCGGGGAAATTCGCGACCGCGAGACCATGGAGCATG
CACTGGCCTTCTCCGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAGACCACCGATGGCGGCCGCCGGGCCGCGGTAGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACTTTCGACAGCGCCCTG
TTCGACTTGGTGGTGGAGGGAGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTATGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCTACCGGCTGGAGCCTCGAACCAACCAAGGACG
ACAAGGGCGACCATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

50.857

92.348

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.36

93.14

0.385
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361