Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L45_RS18820 Genome accession   NZ_CP077363
Coordinates   4070854..4071990 (+) Length   378 a.a.
NCBI ID   WP_043808178.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1415     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4065854..4076990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L45_RS18785 (I6L45_18785) - 4065891..4066271 (-) 381 WP_210550424.1 diacylglycerol kinase -
  I6L45_RS18790 (I6L45_18790) - 4066412..4067479 (+) 1068 WP_134696465.1 3-deoxy-7-phosphoheptulonate synthase -
  I6L45_RS18800 (I6L45_18800) - 4067720..4068505 (-) 786 WP_216982931.1 ATP-binding cassette domain-containing protein -
  I6L45_RS18805 (I6L45_18805) btuC 4068492..4069499 (-) 1008 WP_043759648.1 vitamin B12 ABC transporter permease BtuC -
  I6L45_RS18810 (I6L45_18810) - 4069585..4070334 (-) 750 WP_216982932.1 ABC transporter substrate-binding protein -
  I6L45_RS18815 (I6L45_18815) - 4070443..4070799 (+) 357 WP_025327402.1 DMT family protein -
  I6L45_RS18820 (I6L45_18820) pilU 4070854..4071990 (+) 1137 WP_043808178.1 type IVa pilus ATPase TapW Machinery gene
  I6L45_RS18825 (I6L45_18825) - 4072056..4072298 (-) 243 WP_216982933.1 FeoC-like transcriptional regulator -
  I6L45_RS18830 (I6L45_18830) feoB 4072333..4074603 (-) 2271 WP_216982934.1 Fe(2+) transporter permease subunit FeoB -
  I6L45_RS18835 (I6L45_18835) - 4074814..4075041 (-) 228 WP_216982935.1 FeoA family protein -
  I6L45_RS18840 (I6L45_18840) - 4075161..4075490 (+) 330 WP_043759639.1 translation initiation factor -
  I6L45_RS18845 (I6L45_18845) - 4075732..4076289 (+) 558 WP_043759638.1 TMEM165/GDT1 family protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42172.25 Da        Isoelectric Point: 6.5109

>NTDB_id=580906 I6L45_RS18820 WP_043808178.1 4070854..4071990(+) (pilU) [Aeromonas sp. FDAARGOS 1415]
MELRDMLQMLAKQDGSDLYLSTGAPPCAKFNGALRPLSQALLEPGEVARIADGIMDAEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHHKCIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDDEDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=580906 I6L45_RS18820 WP_043808178.1 4070854..4071990(+) (pilU) [Aeromonas sp. FDAARGOS 1415]
ATGGAATTGAGAGACATGCTACAGATGCTGGCCAAACAGGATGGCTCCGACCTCTATCTCTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCAGCCAGGCCCTGCTCGAACCCGGTGAAGTGGCCCGTATCGCAGACGGCA
TCATGGATGCCGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGC
TTTCGGGTCAATATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAGCTCCCCCCCGTGCTGCTCGATACCATTATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGCACCGGCT
CGGGCAAGTCCACGTCGCTGGCGGCCCTCATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCACAAGTGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTCATCCTCATCGGCGAAATTCGCGATCGCGAAACCATGGAGCATG
CCCTCGCCTTCTCCGAGACCGGGCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAATTTCTTCCCGGAGGAGCGCCGCCCCCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGACGACGGGCGGCGGTGGAGGTGATGCTCGGCACCCACACGGTGCGCGACCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTCGTGGTGGAGGGGGTCATCGACGAGGAGGAGGCGGTGAAGAACGCAGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGCGAGAAGGGGCAGATAGGCAACAGCGCGGCGGCCAGCGGCTGGAGCCTGGAACCCACCAAGGACG
ATGAAGATCTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.667

99.206

0.542
  pilU Acinetobacter baylyi ADP1

53.803

93.915

0.505
  pilU Vibrio cholerae strain A1552

50.142

93.386

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.741

92.857

0.378
  pilT Vibrio cholerae strain A1552

40.741

92.857

0.378
  pilT Pseudomonas aeruginosa PAK

40.698

91.005

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

89.683

0.37
  pilT Legionella pneumophila strain ERS1305867

41.916

88.36

0.37
  pilT Legionella pneumophila strain Lp02

41.916

88.36

0.37
  pilT Pseudomonas stutzeri DSM 10701

40.407

91.005

0.368
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365