Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L35_RS13465 Genome accession   NZ_CP077311
Coordinates   2883020..2884159 (-) Length   379 a.a.
NCBI ID   WP_216978444.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2878020..2889159
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L35_RS13445 (I6L35_13445) oppF 2878768..2879763 (+) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  I6L35_RS13450 (I6L35_13450) - 2880067..2881011 (+) 945 WP_005337664.1 TerC family protein -
  I6L35_RS13455 (I6L35_13455) alr 2881076..2882302 (-) 1227 WP_103422279.1 alanine racemase -
  I6L35_RS13460 (I6L35_13460) - 2882476..2882925 (+) 450 WP_100646207.1 isoprenylcysteine carboxylmethyltransferase family protein -
  I6L35_RS13465 (I6L35_13465) pilU 2883020..2884159 (-) 1140 WP_216978444.1 type IVa pilus ATPase TapW Machinery gene
  I6L35_RS13470 (I6L35_13470) - 2884219..2884575 (-) 357 WP_005353120.1 DMT family protein -
  I6L35_RS13475 (I6L35_13475) - 2884688..2885419 (+) 732 WP_216961916.1 ABC transporter substrate-binding protein -
  I6L35_RS13480 (I6L35_13480) btuC 2885517..2886524 (+) 1008 WP_216961918.1 vitamin B12 ABC transporter permease BtuC -
  I6L35_RS13485 (I6L35_13485) - 2886590..2887387 (+) 798 WP_216963807.1 ATP-binding cassette domain-containing protein -
  I6L35_RS13490 (I6L35_13490) aac(6') 2887410..2887868 (-) 459 WP_216961920.1 aminoglycoside 6'-N-acetyltransferase -
  I6L35_RS13495 (I6L35_13495) - 2887875..2888939 (-) 1065 WP_213386391.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42297.35 Da        Isoelectric Point: 6.4290

>NTDB_id=580759 I6L35_RS13465 WP_216978444.1 2883020..2884159(-) (pilU) [Aeromonas sp. FDAARGOS 1405]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTDIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=580759 I6L35_RS13465 WP_216978444.1 2883020..2884159(-) (pilU) [Aeromonas sp. FDAARGOS 1405]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGAGTTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCGCGCAACATCAAGACCGACATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGTGGCCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCATCGCCATCGCAAGAGCATCATCAACCAGCGGGAAGTGGGCGTGGATACCCGCAGCTTCCATGC
GGCCCTGAAAAACACCCTGCGTCAGGCGCCAGACGTCATTCTTATCGGCGAGATCCGTGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCAATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGTGAATTTGGCGGCCTCAAAGAGGTGATGGAAAAATCCAAAGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTTGATCTGGTGGTAGAAGGCGTGATCGACGAGGAGGAGGCCGTGAAGAACGCCGATTCTGCCAACAACCTGCGTCTCAA
GATCAAACTGTGGAAGGAGAAGGGGCAGATCGCCAGCAGCAGTGATGCCACCGGCTGGAGTCTCGAACCCACCAAGGACG
AGAAAGACGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.36

93.14

0.385
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

87.863

0.369
  pilT Vibrio cholerae strain A1552

42.042

87.863

0.369
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367