Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L46_RS09475 Genome accession   NZ_CP077301
Coordinates   2151516..2152655 (+) Length   379 a.a.
NCBI ID   WP_005361600.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1416     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2146516..2157655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L46_RS09445 (I6L46_09445) - 2146738..2147802 (+) 1065 WP_213386391.1 3-deoxy-7-phosphoheptulonate synthase -
  I6L46_RS09450 (I6L46_09450) aac(6') 2147809..2148267 (+) 459 WP_216977954.1 aminoglycoside 6'-N-acetyltransferase -
  I6L46_RS09455 (I6L46_09455) - 2148290..2149087 (-) 798 WP_216978037.1 ATP-binding cassette domain-containing protein -
  I6L46_RS09460 (I6L46_09460) btuC 2149153..2150160 (-) 1008 WP_216977955.1 vitamin B12 ABC transporter permease BtuC -
  I6L46_RS09465 (I6L46_09465) - 2150258..2150989 (-) 732 WP_216977956.1 ABC transporter substrate-binding protein -
  I6L46_RS09470 (I6L46_09470) - 2151102..2151458 (+) 357 WP_005337656.1 DMT family protein -
  I6L46_RS09475 (I6L46_09475) pilU 2151516..2152655 (+) 1140 WP_005361600.1 type IVa pilus ATPase TapW Machinery gene
  I6L46_RS09480 (I6L46_09480) - 2152785..2153234 (-) 450 WP_005361602.1 isoprenylcysteine carboxylmethyltransferase family protein -
  I6L46_RS09485 (I6L46_09485) alr 2153408..2154634 (+) 1227 WP_216977957.1 alanine racemase -
  I6L46_RS09490 (I6L46_09490) - 2154700..2155644 (-) 945 WP_005337664.1 TerC family protein -
  I6L46_RS09495 (I6L46_09495) oppF 2155947..2156942 (-) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42239.31 Da        Isoelectric Point: 6.6009

>NTDB_id=580650 I6L46_RS09475 WP_005361600.1 2151516..2152655(+) (pilU) [Aeromonas sp. FDAARGOS 1416]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTDIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=580650 I6L46_RS09475 WP_005361600.1 2151516..2152655(+) (pilU) [Aeromonas sp. FDAARGOS 1416]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAGTTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGACATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTACTGTTTGTCGGCGGTACCGGCT
CGGGTAAATCCACTTCGCTGGCGGCGCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTTCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
AGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGTGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATT
ATCAACTTCTTCCCCGAGGAGCGCCGCCCGCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ATTGGTGAAAACCACCGATGGTGGCCGCCGGGCCGCGGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGTGAATTTGGCGGCCTCAAAGAGGTGATGGAAAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTTGATCTGGTGGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367