NTDB

Overview

Name	pilA/pilAI	Type	Machinery gene
Locus tag	KM579_RS16025	Genome accession	NZ_CP076252
Coordinates	3604924..3605334 (-)	Length	136 a.a.
NCBI ID	WP_039955568.1	Uniprot ID	A0A1M4L1M0
Organism	Xanthomonas translucens pv. graminis strain ART-Xtg9
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3599924..3610334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KM579_RS15990 (KM579_15875)	-	3600435..3600731 (+)	297	WP_009580424.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KM579_RS15995 (KM579_15880)	-	3600734..3601027 (+)	294	WP_009580422.1	addiction module antidote protein	-
KM579_RS16000 (KM579_15885)	pilB	3601055..3602791 (-)	1737	WP_009580420.1	type IV-A pilus assembly ATPase PilB	Machinery gene
KM579_RS16005 (KM579_15890)	-	3602998..3603288 (+)	291	WP_009580418.1	nucleotidyltransferase family protein	-
KM579_RS16010 (KM579_15895)	-	3603285..3603605 (+)	321	WP_009580416.1	DUF86 domain-containing protein	-
KM579_RS16015	-	3603705..3603956 (+)	252	WP_009580414.1	transposase	-
KM579_RS16020	-	3603957..3604262 (+)	306	WP_231103532.1	hypothetical protein	-
KM579_RS16025 (KM579_15905)	pilA/pilAI	3604924..3605334 (-)	411	WP_039955568.1	pilin	Machinery gene
KM579_RS16030 (KM579_15910)	pilC	3605724..3606983 (+)	1260	WP_074379066.1	type II secretion system F family protein	Machinery gene
KM579_RS16035 (KM579_15915)	-	3606990..3607853 (+)	864	WP_009580408.1	prepilin peptidase	-
KM579_RS16040 (KM579_15920)	coaE	3607867..3608487 (+)	621	WP_074379065.1	dephospho-CoA kinase	-
KM579_RS16045 (KM579_15925)	-	3608610..3609944 (-)	1335	WP_009580404.1	sensor histidine kinase	-

Sequence

Protein

Download Length: 136 a.a. Molecular weight: 14145.58 Da Isoelectric Point: 8.4919

                                >NTDB_id=574060 KM579_RS16025 WP_039955568.1 3604924..3605334(-) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain ART-Xtg9]

MKKQQGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVTAGLAEINPGKAQYEVALNEGKTAITGIGDLGLKSPTDRCT

ITDITALSPTGSIVCTLKGNTQVLGKTVTLKRNTDGSWTCATTVLAKHAPAGCPGV

Nucleotide

Download Length: 411 bp

                                >NTDB_id=574060 KM579_RS16025 WP_039955568.1 3604924..3605334(-) (pilA/pilAI) [Xanthomonas translucens pv. graminis strain ART-Xtg9]

ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTCATTGCGATCATCGCCATTTTGGCCGCCATTGCGCT

GCCGATGTACCAGGATTATGTCGCCAAGTCGCAAGTAACCGCTGGTCTTGCTGAGATCAACCCGGGTAAGGCCCAGTACG

AAGTTGCATTGAACGAAGGTAAGACCGCTATTACCGGCATTGGCGATCTTGGTTTGAAGTCTCCTACTGACCGTTGCACT

ATCACGGACATTACTGCGCTGAGTCCCACGGGAAGCATCGTTTGTACTTTGAAGGGTAACACCCAGGTTCTCGGCAAGAC

GGTTACCCTGAAACGTAATACTGATGGTTCCTGGACTTGTGCTACCACTGTTTTGGCGAAGCACGCTCCGGCCGGTTGCC

CGGGTGTTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A1M4L1M0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	52.593	99.265	0.522
pilA	Acinetobacter baumannii strain A118	51.095	100	0.515
pilA	Pseudomonas aeruginosa PAK	40.94	100	0.449
pilA/pilAII	Pseudomonas stutzeri DSM 10701	45.522	98.529	0.449
pilA2	Legionella pneumophila str. Paris	43.796	100	0.441
pilA2	Legionella pneumophila strain ERS1305867	43.066	100	0.434
pilA	Vibrio cholerae C6706	41.429	100	0.426
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	41.429	100	0.426
pilA	Vibrio cholerae strain A1552	41.429	100	0.426
pilE	Neisseria gonorrhoeae MS11	40.741	99.265	0.404
comP	Acinetobacter baylyi ADP1	35.57	100	0.39

Detailed information NTG0574060