Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KM579_RS16000 Genome accession   NZ_CP076252
Coordinates   3601055..3602791 (-) Length   578 a.a.
NCBI ID   WP_009580420.1    Uniprot ID   A0A1M4J5J2
Organism   Xanthomonas translucens pv. graminis strain ART-Xtg9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3596055..3607791
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM579_RS15980 (KM579_15865) - 3597194..3598807 (+) 1614 WP_009580428.1 sensor histidine kinase -
  KM579_RS15985 (KM579_15870) pilR 3598882..3600270 (+) 1389 WP_009580426.1 sigma-54-dependent transcriptional regulator Regulator
  KM579_RS15990 (KM579_15875) - 3600435..3600731 (+) 297 WP_009580424.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KM579_RS15995 (KM579_15880) - 3600734..3601027 (+) 294 WP_009580422.1 addiction module antidote protein -
  KM579_RS16000 (KM579_15885) pilB 3601055..3602791 (-) 1737 WP_009580420.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KM579_RS16005 (KM579_15890) - 3602998..3603288 (+) 291 WP_009580418.1 nucleotidyltransferase family protein -
  KM579_RS16010 (KM579_15895) - 3603285..3603605 (+) 321 WP_009580416.1 DUF86 domain-containing protein -
  KM579_RS16015 - 3603705..3603956 (+) 252 WP_009580414.1 transposase -
  KM579_RS16020 - 3603957..3604262 (+) 306 WP_231103532.1 hypothetical protein -
  KM579_RS16025 (KM579_15905) pilA/pilAI 3604924..3605334 (-) 411 WP_039955568.1 pilin Machinery gene
  KM579_RS16030 (KM579_15910) pilC 3605724..3606983 (+) 1260 WP_074379066.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62918.20 Da        Isoelectric Point: 6.1466

>NTDB_id=574059 KM579_RS16000 WP_009580420.1 3601055..3602791(-) (pilB) [Xanthomonas translucens pv. graminis strain ART-Xtg9]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELLHKYQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRVLDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=574059 KM579_RS16000 WP_009580420.1 3601055..3602791(-) (pilB) [Xanthomonas translucens pv. graminis strain ART-Xtg9]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGTTGCACAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATTTGACGGTCGAGCCGA
TCCTGGTCGATGAGGACCAGATCCGCCGCACCCTGGAACAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGTGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCGTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCCGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTGTCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAGATCCGTGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACACTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGCCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCTGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATTGCGGCGATCGTAT
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGATCTGCGCCAGTCGGCGCTGCTC
AAGGCACGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1M4J5J2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Acinetobacter baumannii D1279779

53.927

99.135

0.535
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.047

99.827

0.49
  pilF Neisseria gonorrhoeae MS11

48.592

98.27

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

97.924

0.458
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.421

90.311

0.365
  pilF Thermus thermophilus HB27

40.385

89.965

0.363