Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA2   Type   Machinery gene
Locus tag   AZKH_RS19430 Genome accession   NC_020516
Coordinates   4348237..4348662 (+) Length   141 a.a.
NCBI ID   WP_015437506.1    Uniprot ID   H0PUD0
Organism   Azoarcus sp. KH32C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 4343237..4353662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AZKH_RS19425 (AZKH_3919) - 4344307..4347471 (+) 3165 WP_015437505.1 efflux RND transporter permease subunit -
  AZKH_RS19430 (AZKH_3920) pilA2 4348237..4348662 (+) 426 WP_015437506.1 pilin Machinery gene
  AZKH_RS19435 (AZKH_3921) - 4348727..4349062 (+) 336 WP_015437507.1 hypothetical protein -
  AZKH_RS26415 (AZKH_3922) - 4349127..4352069 (+) 2943 WP_083903104.1 glycosyltransferase -
  AZKH_RS19445 (AZKH_3923) - 4352178..4352957 (+) 780 WP_015437509.1 TylF/MycF/NovP-related O-methyltransferase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 14044.28 Da        Isoelectric Point: 8.4883

>NTDB_id=56724 AZKH_RS19430 WP_015437506.1 4348237..4348662(+) (pilA2) [Azoarcus sp. KH32C]
MKKIQQGFTLIELMIVVAIIGILAAVALPAYQNYTIRAKVSELVLAASGARTCITEVYQSVGGATIPTTVAADCAVSTVG
KVTGATVSTGGVVTVTGTTATSSVGAAVTITLTPTVQSAAGTLTWACSGSDTKYVPASCRG

Nucleotide


Download         Length: 426 bp        

>NTDB_id=56724 AZKH_RS19430 WP_015437506.1 4348237..4348662(+) (pilA2) [Azoarcus sp. KH32C]
ATGAAAAAGATCCAACAAGGCTTTACCCTCATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCCGCCGTCGC
GCTGCCGGCTTACCAGAACTACACGATCCGGGCCAAGGTTTCGGAACTGGTGCTGGCCGCCAGCGGCGCGCGCACCTGCA
TCACCGAGGTTTACCAGTCGGTCGGGGGAGCGACGATCCCAACGACGGTTGCAGCGGACTGCGCAGTCTCGACCGTCGGC
AAGGTAACCGGTGCGACGGTGTCGACTGGTGGGGTCGTCACGGTCACGGGCACTACTGCCACAAGCTCCGTGGGCGCGGC
CGTGACCATCACCCTGACGCCGACAGTCCAGTCGGCCGCAGGCACCCTGACCTGGGCTTGCTCGGGCTCCGACACCAAGT
ACGTCCCGGCCTCCTGCCGCGGCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB H0PUD0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

48.571

99.291

0.482

  comP Acinetobacter baylyi ADP1

44.667

100

0.475

  pilA2 Legionella pneumophila strain ERS1305867

47.857

99.291

0.475

  pilA/pilA1 Eikenella corrodens VA1

40.26

100

0.44

  pilE Neisseria gonorrhoeae strain FA1090

38.75

100

0.44

  pilE Neisseria gonorrhoeae MS11

38.509

100

0.44

  pilA Ralstonia pseudosolanacearum GMI1000

35.503

100

0.426

  pilA Acinetobacter baumannii strain A118

38.514

100

0.404

  pilA Vibrio parahaemolyticus RIMD 2210633

42.636

91.489

0.39

  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.242

100

0.383

  pilA Glaesserella parasuis strain SC1401

35.526

100

0.383

  pilA/pilAII Pseudomonas stutzeri DSM 10701

34.211

100

0.369

  pilA Pseudomonas aeruginosa PAK

33.548

100

0.369


Multiple sequence alignment