Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KAF26_RS05235 Genome accession   NZ_CP074363
Coordinates   1208208..1208666 (+) Length   152 a.a.
NCBI ID   WP_003466389.1    Uniprot ID   -
Organism   Xanthomonas translucens pv. secalis strain CFBP 2539     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1203208..1213666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAF26_RS05215 (KAF26_05140) - 1203598..1204932 (+) 1335 WP_003477107.1 HAMP domain-containing sensor histidine kinase -
  KAF26_RS05220 (KAF26_05145) coaE 1205059..1205679 (-) 621 WP_003466399.1 dephospho-CoA kinase -
  KAF26_RS05225 (KAF26_05150) - 1205693..1206556 (-) 864 WP_003466396.1 prepilin peptidase -
  KAF26_RS05230 (KAF26_05155) pilC 1206563..1207822 (-) 1260 WP_003466392.1 type II secretion system F family protein Machinery gene
  KAF26_RS05235 (KAF26_05160) pilE 1208208..1208666 (+) 459 WP_003466389.1 pilin Machinery gene
  KAF26_RS05240 (KAF26_05165) pilB 1209398..1211134 (+) 1737 WP_003466384.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KAF26_RS05245 (KAF26_05170) - 1211309..1211761 (-) 453 WP_230811680.1 Fic family protein -
  KAF26_RS05250 (KAF26_05175) - 1211758..1211940 (-) 183 WP_003466380.1 YhfG family protein -
  KAF26_RS05255 (KAF26_05180) pilR 1212129..1213517 (-) 1389 WP_003466378.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15348.56 Da        Isoelectric Point: 8.4798

>NTDB_id=564264 KAF26_RS05235 WP_003466389.1 1208208..1208666(+) (pilE) [Xanthomonas translucens pv. secalis strain CFBP 2539]
MKKQQGFTLIELMIVVAIIAILAAIAIPAYQDYIARAQMSEALTLASGQKVGVSEVFSNKGTCPANGSDGFAADTDINGK
YVQKVTVGGTAAASGGCTIVATMKSTGVSTGVQGATLTLTLSNADKGSNVWTCTSSAKQKYLPTACTGSTGA

Nucleotide


Download         Length: 459 bp        

>NTDB_id=564264 KAF26_RS05235 WP_003466389.1 1208208..1208666(+) (pilE) [Xanthomonas translucens pv. secalis strain CFBP 2539]
ATGAAGAAGCAACAGGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATTGCAAT
CCCGGCTTATCAGGATTATATCGCTCGTGCACAAATGTCCGAGGCGCTGACTTTGGCGAGCGGCCAGAAGGTCGGTGTCT
CTGAGGTTTTTTCTAATAAGGGAACTTGCCCGGCCAATGGCAGCGACGGATTCGCCGCTGACACTGACATCAATGGCAAG
TATGTACAAAAGGTTACGGTGGGTGGTACTGCTGCAGCTTCCGGTGGGTGCACGATTGTTGCAACCATGAAGAGCACCGG
CGTTTCCACTGGTGTTCAGGGTGCAACTCTCACCTTGACCCTTTCGAACGCTGACAAGGGTTCTAATGTCTGGACATGCA
CGTCCAGCGCTAAGCAAAAATATCTGCCGACGGCTTGTACCGGCAGTACCGGCGCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

44.91

100

0.493
  pilE Neisseria gonorrhoeae MS11

45.062

100

0.48
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.881

100

0.428
  comP Acinetobacter baylyi ADP1

42.763

100

0.428
  pilA Ralstonia pseudosolanacearum GMI1000

39.264

100

0.421
  pilA Acinetobacter nosocomialis M2

50.82

80.263

0.408
  pilA2 Legionella pneumophila strain ERS1305867

42.466

96.053

0.408
  pilA Pseudomonas aeruginosa PAK

39.49

100

0.408
  pilA2 Legionella pneumophila str. Paris

42.069

95.395

0.401
  pilA Acinetobacter baumannii strain A118

39.474

100

0.395
  pilA/pilA1 Eikenella corrodens VA1

35.22

100

0.368
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

96.053

0.368