Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   J4854_RS01135 Genome accession   NZ_CP072329
Coordinates   194641..195582 (+) Length   313 a.a.
NCBI ID   WP_200772863.1    Uniprot ID   -
Organism   Streptococcus lactarius strain CCUG 66490     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 189641..200582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4854_RS01110 (J4854_01110) - 190584..191372 (+) 789 WP_200772860.1 hypothetical protein -
  J4854_RS01115 (J4854_01115) - 191382..191522 (+) 141 WP_187539697.1 hypothetical protein -
  J4854_RS01120 (J4854_01120) - 191515..192825 (+) 1311 WP_200772861.1 glycosyltransferase family 2 protein -
  J4854_RS01125 (J4854_01125) - 192834..193937 (+) 1104 WP_200772862.1 glycosyl hydrolase family 8 -
  J4854_RS01130 (J4854_01130) - 194166..194555 (+) 390 WP_200772958.1 DUF1033 family protein -
  J4854_RS01135 (J4854_01135) comYA 194641..195582 (+) 942 WP_200772863.1 competence type IV pilus ATPase ComGA Machinery gene
  J4854_RS01140 (J4854_01140) comYB 195515..196546 (+) 1032 WP_244967046.1 competence type IV pilus assembly protein ComGB Machinery gene
  J4854_RS01145 (J4854_01145) comYC 196543..196860 (+) 318 WP_187539705.1 competence type IV pilus major pilin ComGC Machinery gene
  J4854_RS01150 (J4854_01150) comYD 196850..197254 (+) 405 WP_234700114.1 competence type IV pilus minor pilin ComGD Machinery gene
  J4854_RS01155 (J4854_01155) comGE 197295..197507 (+) 213 WP_234700115.1 competence type IV pilus minor pilin ComGE -
  J4854_RS01160 (J4854_01160) comGF/cglF 197497..197949 (+) 453 WP_200772864.1 competence type IV pilus minor pilin ComGF Machinery gene
  J4854_RS01165 (J4854_01165) comGG 197915..198382 (+) 468 WP_200772865.1 competence type IV pilus minor pilin ComGG -
  J4854_RS01170 (J4854_01170) comYH 198413..199366 (+) 954 WP_200772866.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35741.09 Da        Isoelectric Point: 6.5074

>NTDB_id=552017 J4854_RS01135 WP_200772863.1 194641..195582(+) (comYA) [Streptococcus lactarius strain CCUG 66490]
MVQEIAKKLIRMGKKEEAQDLYFIPRSEGYQVFMRVGDERRFVQTFPFEEMTAIISHFKFVAGMNVGEKRRSQLGSCDYP
LEEGVVSIRLSTVGDYRGYESLVIRLLHDEERELRFWFDQLPELQKKLAGRGLYLFAGPVGSGKTTLMHALAQARFADQQ
VMSIEDPVEIKQDNMLQLQLNDQIGMTYDNLIKLSLRHRPDLLIIGEIRDKETARAVVRASLTGVTVFSTIHAKSVRGVY
ERLLELGVSEDELKIVLQGICYQRLIAGGGVVDFATENYQEHKASRWNQQMDLLAQSGYIQLAQAQAEKIIYN

Nucleotide


Download         Length: 942 bp        

>NTDB_id=552017 J4854_RS01135 WP_200772863.1 194641..195582(+) (comYA) [Streptococcus lactarius strain CCUG 66490]
ATGGTTCAAGAAATTGCAAAAAAATTGATTCGCATGGGAAAGAAGGAAGAAGCCCAGGATCTTTATTTTATCCCTCGAAG
TGAGGGATACCAAGTCTTTATGAGGGTGGGAGATGAGAGGCGCTTTGTACAGACCTTTCCTTTTGAGGAGATGACAGCGA
TCATCAGCCACTTTAAATTCGTAGCAGGGATGAATGTGGGTGAAAAAAGACGCAGTCAATTGGGCTCGTGTGACTACCCT
TTGGAAGAGGGGGTGGTCTCGATCCGTTTGTCAACGGTTGGGGATTATAGAGGCTATGAAAGCTTGGTAATTCGTCTCCT
ACATGATGAGGAGCGGGAGTTGCGCTTCTGGTTTGACCAGTTGCCGGAATTGCAGAAAAAGTTAGCAGGTCGGGGGCTCT
ATCTTTTTGCGGGGCCTGTTGGTTCGGGCAAGACCACCCTCATGCATGCTTTAGCGCAGGCCCGTTTTGCGGACCAGCAA
GTCATGTCTATTGAAGATCCTGTTGAGATCAAGCAGGACAATATGCTCCAGCTCCAGCTCAATGATCAGATTGGCATGAC
CTATGACAACTTGATTAAACTCTCCCTACGCCATCGACCGGATCTTCTCATCATTGGGGAGATCCGAGATAAGGAAACCG
CTCGTGCAGTGGTACGAGCTAGTTTGACAGGTGTGACGGTCTTCTCCACTATTCACGCCAAGAGTGTTAGAGGAGTCTAT
GAGCGCCTCTTGGAGCTTGGGGTGAGTGAGGATGAGCTAAAGATTGTCTTACAAGGAATTTGCTACCAACGATTAATTGC
AGGAGGAGGTGTTGTTGATTTTGCAACGGAAAACTACCAAGAACACAAAGCCAGCCGCTGGAACCAGCAAATGGATCTCT
TGGCTCAATCAGGATATATCCAGCTGGCTCAGGCACAAGCCGAAAAAATTATCTACAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

73.871

99.042

0.732
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

71.565

100

0.716
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

70.927

100

0.709
  comGA/cglA/cilD Streptococcus pneumoniae D39

70.927

100

0.709
  comGA/cglA/cilD Streptococcus pneumoniae R6

70.927

100

0.709
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

70.927

100

0.709
  comYA Streptococcus mutans UA159

64.309

99.361

0.639
  comYA Streptococcus mutans UA140

64.309

99.361

0.639
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

54.14

100

0.543
  comGA Latilactobacillus sakei subsp. sakei 23K

42.446

88.818

0.377