NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	J4854_RS01160	Genome accession	NZ_CP072329
Coordinates	197497..197949 (+)	Length	150 a.a.
NCBI ID	WP_200772864.1	Uniprot ID	A0A9X1BCX6
Organism	Streptococcus lactarius strain CCUG 66490
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 192497..202949

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J4854_RS01125 (J4854_01125)	-	192834..193937 (+)	1104	WP_200772862.1	glycosyl hydrolase family 8	-
J4854_RS01130 (J4854_01130)	-	194166..194555 (+)	390	WP_200772958.1	DUF1033 family protein	-
J4854_RS01135 (J4854_01135)	comYA	194641..195582 (+)	942	WP_200772863.1	competence type IV pilus ATPase ComGA	Machinery gene
J4854_RS01140 (J4854_01140)	comYB	195515..196546 (+)	1032	WP_244967046.1	competence type IV pilus assembly protein ComGB	Machinery gene
J4854_RS01145 (J4854_01145)	comYC	196543..196860 (+)	318	WP_187539705.1	competence type IV pilus major pilin ComGC	Machinery gene
J4854_RS01150 (J4854_01150)	comYD	196850..197254 (+)	405	WP_234700114.1	competence type IV pilus minor pilin ComGD	Machinery gene
J4854_RS01155 (J4854_01155)	comGE	197295..197507 (+)	213	WP_234700115.1	competence type IV pilus minor pilin ComGE	-
J4854_RS01160 (J4854_01160)	comGF/cglF	197497..197949 (+)	453	WP_200772864.1	competence type IV pilus minor pilin ComGF	Machinery gene
J4854_RS01165 (J4854_01165)	comGG	197915..198382 (+)	468	WP_200772865.1	competence type IV pilus minor pilin ComGG	-
J4854_RS01170 (J4854_01170)	comYH	198413..199366 (+)	954	WP_200772866.1	class I SAM-dependent methyltransferase	Machinery gene
J4854_RS01175 (J4854_01175)	-	199418..200611 (+)	1194	WP_200772867.1	acetate kinase	-
J4854_RS01180 (J4854_01180)	folP	201028..201987 (+)	960	WP_200772868.1	dihydropteroate synthase	-

Sequence

Protein

Download Length: 150 a.a. Molecular weight: 17525.03 Da Isoelectric Point: 6.2304

                                >NTDB_id=552021 J4854_RS01160 WP_200772864.1 197497..197949(+) (comGF/cglF) [Streptococcus lactarius strain CCUG 66490]

MWSKRKVKAFTLLEALVALLVLSGGILIFQGLTKLLHSELDYQAHQKQEEWLLFQQQLQVELDRSHVEKVENNHIYLTQD

QKPIAIGQSKEDDIRKTDDRGRGYQPMIAGVRSSKIWQEEDLIHVQISFEEGLEREYIYHVVPAIQKEKS

Nucleotide

Download Length: 453 bp

                                >NTDB_id=552021 J4854_RS01160 WP_200772864.1 197497..197949(+) (comGF/cglF) [Streptococcus lactarius strain CCUG 66490]

ATGTGGAGTAAACGAAAGGTCAAAGCTTTCACCCTACTCGAAGCCTTGGTCGCTCTGTTGGTGCTTAGTGGCGGTATCCT

GATCTTTCAAGGATTGACCAAACTCTTGCATTCGGAGCTGGACTACCAAGCTCACCAAAAACAGGAAGAGTGGCTGTTGT

TTCAACAACAATTGCAGGTAGAATTGGATCGCAGTCATGTTGAAAAAGTGGAGAACAATCACATCTACTTGACTCAAGAT

CAAAAACCAATTGCGATTGGCCAGTCTAAGGAAGATGATATTCGGAAAACGGATGATCGGGGTAGGGGGTACCAACCCAT

GATTGCAGGTGTTCGCTCTAGTAAAATATGGCAGGAAGAAGATCTCATTCATGTGCAGATAAGCTTTGAAGAAGGCCTAG

AAAGGGAGTATATCTACCATGTGGTTCCAGCGATTCAAAAAGAAAAGAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(42-134)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	57.931	96.667	0.56
comGF/cglF	Streptococcus pneumoniae D39	57.931	96.667	0.56
comGF/cglF	Streptococcus pneumoniae R6	57.931	96.667	0.56
comGF/cglF	Streptococcus pneumoniae TIGR4	57.931	96.667	0.56
comGF/cglF	Streptococcus mitis NCTC 12261	56.115	92.667	0.52
comGF/cglF	Streptococcus mitis SK321	55.396	92.667	0.513
comGF	Lactococcus lactis subsp. cremoris KW2	39.716	94	0.373
comYF	Streptococcus mutans UA159	40.441	90.667	0.367
comYF	Streptococcus mutans UA140	40.441	90.667	0.367

Detailed information NTG0552021