NTDB

Overview

Name	comYB	Type	Machinery gene
Locus tag	J4854_RS01140	Genome accession	NZ_CP072329
Coordinates	195515..196546 (+)	Length	343 a.a.
NCBI ID	WP_244967046.1	Uniprot ID	-
Organism	Streptococcus lactarius strain CCUG 66490
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 190515..201546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J4854_RS01110 (J4854_01110)	-	190584..191372 (+)	789	WP_200772860.1	hypothetical protein	-
J4854_RS01115 (J4854_01115)	-	191382..191522 (+)	141	WP_187539697.1	hypothetical protein	-
J4854_RS01120 (J4854_01120)	-	191515..192825 (+)	1311	WP_200772861.1	glycosyltransferase family 2 protein	-
J4854_RS01125 (J4854_01125)	-	192834..193937 (+)	1104	WP_200772862.1	glycosyl hydrolase family 8	-
J4854_RS01130 (J4854_01130)	-	194166..194555 (+)	390	WP_200772958.1	DUF1033 family protein	-
J4854_RS01135 (J4854_01135)	comYA	194641..195582 (+)	942	WP_200772863.1	competence type IV pilus ATPase ComGA	Machinery gene
J4854_RS01140 (J4854_01140)	comYB	195515..196546 (+)	1032	WP_244967046.1	competence type IV pilus assembly protein ComGB	Machinery gene
J4854_RS01145 (J4854_01145)	comYC	196543..196860 (+)	318	WP_187539705.1	competence type IV pilus major pilin ComGC	Machinery gene
J4854_RS01150 (J4854_01150)	comYD	196850..197254 (+)	405	WP_234700114.1	competence type IV pilus minor pilin ComGD	Machinery gene
J4854_RS01155 (J4854_01155)	comGE	197295..197507 (+)	213	WP_234700115.1	competence type IV pilus minor pilin ComGE	-
J4854_RS01160 (J4854_01160)	comGF/cglF	197497..197949 (+)	453	WP_200772864.1	competence type IV pilus minor pilin ComGF	Machinery gene
J4854_RS01165 (J4854_01165)	comGG	197915..198382 (+)	468	WP_200772865.1	competence type IV pilus minor pilin ComGG	-
J4854_RS01170 (J4854_01170)	comYH	198413..199366 (+)	954	WP_200772866.1	class I SAM-dependent methyltransferase	Machinery gene
J4854_RS01175 (J4854_01175)	-	199418..200611 (+)	1194	WP_200772867.1	acetate kinase	-

Sequence

Protein

Download Length: 343 a.a. Molecular weight: 39083.97 Da Isoelectric Point: 9.1834

                                >NTDB_id=552018 J4854_RS01140 WP_244967046.1 195515..196546(+) (comYB) [Streptococcus lactarius strain CCUG 66490]

MSWLNQDISSWLRHKPKKLSTTKQKQIIELFSNLYSSGFHLAEIVDFLDRSHLVDGRLVAKMREDLAQGRRFSEMMAGIG

FSDAVTTQLSLAEHHGNIELSLEKIGAYLENMRKVKKKLIEVSTYPLLLVGFLILIMLGLRNYLLPQMDSQNIGTQLISL

FPQIFLALVAGSGILVLLGVAYYRKSGKIKVFSALSHLPFGKGMIQAYLTAYYAREWGNMIGQGLELSQIFSMMQEQKSQ

LFQEIGVDLVLSLDRGQSFSETVGHYPFFKKELPLMIEYGEVKSKLGSELEIYAEKTWEDFFRRVHKAMNVIQPLVFVFV

ALVIVLLYAAMLLPIYQNMEVHL

Nucleotide

Download Length: 1032 bp

                                >NTDB_id=552018 J4854_RS01140 WP_244967046.1 195515..196546(+) (comYB) [Streptococcus lactarius strain CCUG 66490]

ATCTCTTGGCTCAATCAGGATATATCCAGCTGGCTCAGGCACAAGCCGAAAAAATTATCTACAACTAAACAAAAGCAAAT

CATTGAATTGTTTTCCAATCTTTACTCGAGTGGTTTTCACCTAGCTGAAATTGTGGATTTCCTAGACCGTTCCCATCTGG

TGGATGGGCGCTTGGTAGCTAAGATGCGGGAGGATCTTGCTCAAGGACGACGCTTCTCTGAGATGATGGCGGGAATTGGT

TTTTCAGATGCGGTGACCACACAGTTATCTTTAGCAGAGCACCACGGGAACATTGAATTGAGCTTAGAGAAAATCGGAGC

TTATTTGGAAAATATGCGCAAGGTCAAGAAAAAATTAATTGAGGTAAGTACCTATCCTCTTCTTTTAGTCGGATTTTTAA

TCCTCATTATGTTGGGCTTGCGCAATTATTTACTTCCTCAGATGGATAGCCAAAATATAGGAACGCAATTGATCAGCCTG

TTTCCGCAAATCTTTTTGGCTCTCGTCGCGGGCAGTGGGATTTTGGTCCTACTAGGAGTGGCTTATTACCGGAAATCTGG

CAAAATCAAAGTATTTAGTGCCCTGTCTCACCTGCCTTTTGGGAAAGGCATGATTCAAGCTTATTTGACGGCTTATTATG

CCAGAGAATGGGGCAATATGATCGGCCAGGGATTAGAGTTATCCCAAATTTTTAGCATGATGCAAGAACAAAAATCTCAG

CTCTTTCAAGAGATTGGAGTGGACTTGGTGCTGTCCTTGGATCGGGGTCAATCTTTTTCAGAGACAGTTGGCCACTATCC

CTTTTTCAAAAAAGAGTTGCCTCTTATGATTGAGTATGGCGAAGTAAAATCAAAACTCGGAAGTGAGCTAGAGATTTATG

CTGAGAAAACCTGGGAGGATTTCTTCCGCCGGGTTCACAAGGCTATGAATGTGATTCAACCCTTGGTCTTTGTCTTTGTG

GCTCTTGTAATTGTATTACTGTATGCAGCTATGTTGCTGCCAATTTATCAAAATATGGAGGTTCATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comYB	Streptococcus gordonii str. Challis substr. CH1	66.377	100	0.668
comGB/cglB	Streptococcus mitis SK321	66.667	97.959	0.653
comGB/cglB	Streptococcus mitis NCTC 12261	66.172	98.251	0.65
comGB/cglB	Streptococcus pneumoniae TIGR4	65.875	98.251	0.647
comGB/cglB	Streptococcus pneumoniae R6	65.875	98.251	0.647
comGB/cglB	Streptococcus pneumoniae Rx1	65.875	98.251	0.647
comGB/cglB	Streptococcus pneumoniae D39	65.875	98.251	0.647
comYB	Streptococcus mutans UA159	57.434	100	0.574
comYB	Streptococcus mutans UA140	57.434	100	0.574
comGB	Lactococcus lactis subsp. cremoris KW2	47.478	98.251	0.466

Detailed information NTG0552018