Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   J3S98_RS03190 Genome accession   NZ_CP071729
Coordinates   666419..667399 (+) Length   326 a.a.
NCBI ID   WP_021164974.1    Uniprot ID   O86279
Organism   Lactococcus cremoris strain FM-YL11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 661419..672399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3S98_RS03185 (J3S98_03185) - 662510..666304 (+) 3795 Protein_604 PolC-type DNA polymerase III -
  J3S98_RS03190 (J3S98_03190) comGA 666419..667399 (+) 981 WP_021164974.1 competence type IV pilus ATPase ComGA Machinery gene
  J3S98_RS03195 (J3S98_03195) comGB 667299..668324 (+) 1026 WP_050574185.1 competence type IV pilus assembly protein ComGB Machinery gene
  J3S98_RS03200 (J3S98_03200) comGC 668369..668719 (+) 351 WP_050574187.1 competence type IV pilus major pilin ComGC Machinery gene
  J3S98_RS12270 comGD 668921..669109 (+) 189 WP_014573336.1 hypothetical protein Machinery gene
  J3S98_RS03210 (J3S98_03210) comGE 669141..669377 (+) 237 WP_014573335.1 competence type IV pilus minor pilin ComGE Machinery gene
  J3S98_RS03215 (J3S98_03215) comGF 669340..669786 (+) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  J3S98_RS03220 (J3S98_03220) comGG 669882..670109 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  J3S98_RS03225 (J3S98_03225) - 670189..670626 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  J3S98_RS03230 (J3S98_03230) - 670623..671465 (+) 843 WP_021164979.1 zinc ABC transporter substrate-binding protein -
  J3S98_RS03235 (J3S98_03235) - 671644..672381 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 37057.37 Da        Isoelectric Point: 5.7372

>NTDB_id=547283 J3S98_RS03190 WP_021164974.1 666419..667399(+) (comGA) [Lactococcus cremoris strain FM-YL11]
MVQKKAQELIQKAIEMEASDIYLIASGNLYKIYIRQQLGRTLIEELNQEIGLALLTHFKFLAGMNTGERRRVQLSACWYE
LEGSSAKRLRLSTVGDFEGNESLVIRLLHDQKQELEFWFDDKLQDFRCKRGLYLFSGPVGSGKTSLMFDLAPRHFSNAQV
ITIEEPVELIESDFIQLQVNDVIGNSYDELIKLSLRHRPDLLIVGEIRDQQTARAVLRASLTGYTFFSTIHAASVKGVVQ
RLLELGLSDWELKNGLEAVVYQRLIAGKGVLDIAKSKFDTWSPKKWNEKIENLYANGHLTAIEAEREKISINQASKIDTT
DGESFE

Nucleotide


Download         Length: 981 bp        

>NTDB_id=547283 J3S98_RS03190 WP_021164974.1 666419..667399(+) (comGA) [Lactococcus cremoris strain FM-YL11]
ATGGTACAGAAAAAAGCACAAGAACTCATTCAAAAGGCAATTGAGATGGAGGCTTCTGATATTTATTTAATTGCTTCAGG
AAATCTTTATAAGATATATATTCGTCAACAATTAGGGCGAACTTTAATAGAGGAACTTAACCAAGAGATTGGTTTAGCAT
TACTTACTCATTTTAAATTTCTTGCTGGCATGAATACTGGTGAACGCCGGCGTGTTCAGCTGAGTGCTTGTTGGTATGAA
CTAGAGGGAAGTTCGGCAAAACGTTTGCGCCTTTCAACAGTGGGAGATTTTGAAGGAAATGAATCATTGGTTATCCGTCT
TTTGCATGACCAAAAACAAGAACTTGAGTTTTGGTTTGACGATAAACTTCAGGATTTTCGATGTAAACGAGGACTTTATT
TATTTTCTGGGCCAGTAGGGTCTGGGAAAACTTCACTAATGTTTGACTTAGCCCCCCGTCATTTTTCAAATGCACAGGTT
ATCACTATTGAGGAGCCTGTAGAATTAATTGAATCTGATTTTATTCAGTTACAAGTTAATGATGTAATTGGGAATAGTTA
TGATGAATTAATTAAACTTTCTTTAAGACACCGACCAGATTTACTAATTGTTGGAGAAATTCGAGATCAGCAGACAGCTC
GTGCTGTATTGCGTGCAAGTTTAACAGGCTATACATTTTTTTCTACTATCCATGCGGCTTCCGTGAAGGGAGTGGTTCAA
CGTTTGTTGGAATTGGGTTTGAGTGATTGGGAATTGAAAAATGGATTAGAGGCAGTTGTTTATCAACGGTTAATAGCGGG
AAAAGGAGTATTAGATATTGCGAAAAGTAAATTTGACACTTGGTCGCCGAAAAAATGGAATGAAAAGATTGAGAACTTAT
ATGCAAACGGACATCTTACGGCTATTGAGGCCGAAAGGGAAAAAATTAGCATTAATCAAGCAAGCAAAATTGATACAACT
GATGGGGAATCTTTTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O86279

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

98.16

100

0.982
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.806

95.092

0.531
  comYA Streptococcus gordonii str. Challis substr. CH1

53.797

96.933

0.521
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.846

95.706

0.515
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.846

95.706

0.515
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.846

95.706

0.515
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.846

95.706

0.515
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.846

95.706

0.515
  comYA Streptococcus mutans UA159

52.09

95.399

0.497
  comYA Streptococcus mutans UA140

52.09

95.399

0.497