Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   J3S98_RS03215 Genome accession   NZ_CP071729
Coordinates   669340..669786 (+) Length   148 a.a.
NCBI ID   WP_011836043.1    Uniprot ID   A0A1V0PET3
Organism   Lactococcus cremoris strain FM-YL11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 670189..674649 669340..669786 flank 403


Gene organization within MGE regions


Location: 669340..674649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3S98_RS03215 (J3S98_03215) comGF 669340..669786 (+) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  J3S98_RS03220 (J3S98_03220) comGG 669882..670109 (+) 228 WP_228764408.1 competence protein ComGG Machinery gene
  J3S98_RS03225 (J3S98_03225) - 670189..670626 (+) 438 WP_011677180.1 zinc-dependent MarR family transcriptional regulator -
  J3S98_RS03230 (J3S98_03230) - 670623..671465 (+) 843 WP_021164979.1 zinc ABC transporter substrate-binding protein -
  J3S98_RS03235 (J3S98_03235) - 671644..672381 (+) 738 WP_011677178.1 metal ABC transporter ATP-binding protein -
  J3S98_RS03240 (J3S98_03240) - 672374..673183 (+) 810 WP_011677177.1 metal ABC transporter permease -
  J3S98_RS03245 (J3S98_03245) - 673256..674649 (+) 1394 WP_207873735.1 IS3 family transposase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17102.82 Da        Isoelectric Point: 8.2376

>NTDB_id=547287 J3S98_RS03215 WP_011836043.1 669340..669786(+) (comGF) [Lactococcus cremoris strain FM-YL11]
MERKFCDLKLKIRAFTLLECLVALLAISGSVLVISGLTRMIEEQMKISQNDSRKDWQIFCEQMRSELSGAKLDNVNQNFL
YVTKDKKLRFGLVGDDFRKSDDKGQGYQPMLYDLKGAKIQAEENLIKITIDFDNGGERVFIYRFTDTK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=547287 J3S98_RS03215 WP_011836043.1 669340..669786(+) (comGF) [Lactococcus cremoris strain FM-YL11]
ATGGAAAGGAAATTTTGCGACTTGAAGCTCAAAATTAGGGCATTTACTTTACTTGAATGTCTCGTAGCACTTTTGGCAAT
TTCTGGTTCAGTGCTAGTCATCTCAGGGCTGACTAGAATGATTGAAGAACAGATGAAAATAAGCCAGAATGATAGTAGGA
AAGATTGGCAAATATTTTGTGAACAAATGCGTTCAGAACTCTCCGGAGCAAAATTAGACAATGTGAATCAAAATTTTTTG
TATGTGACCAAAGATAAGAAGTTACGGTTCGGATTAGTAGGGGATGATTTTCGTAAAAGTGATGATAAAGGGCAAGGATA
CCAACCGATGCTTTATGATTTAAAAGGAGCAAAAATTCAGGCAGAAGAAAATTTGATAAAAATAACAATTGATTTTGATA
ATGGAGGTGAGCGAGTATTTATTTATCGATTTACTGATACAAAGTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

99.291

95.27

0.946
  comYF Streptococcus mutans UA140

44.681

95.27

0.426
  comYF Streptococcus mutans UA159

43.972

95.27

0.419
  comGF/cglF Streptococcus mitis SK321

39.286

94.595

0.372
  comGF/cglF Streptococcus mitis NCTC 12261

39.286

94.595

0.372