Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JYB88_RS04785 Genome accession   NZ_CP071504
Coordinates   1119281..1120393 (+) Length   370 a.a.
NCBI ID   WP_207322300.1    Uniprot ID   A0A975ALT3
Organism   Shewanella cyperi strain FJAT-53726     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1114281..1125393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JYB88_RS04750 (JYB88_04750) rdgB 1114434..1115033 (-) 600 WP_207325654.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JYB88_RS04755 (JYB88_04755) - 1115132..1115566 (-) 435 WP_207325655.1 DUF4426 domain-containing protein -
  JYB88_RS04760 (JYB88_04760) yggU 1115644..1115931 (-) 288 WP_207325656.1 DUF167 family protein YggU -
  JYB88_RS04765 (JYB88_04765) - 1115931..1116479 (-) 549 WP_207322296.1 YggT family protein -
  JYB88_RS04770 (JYB88_04770) proC 1116520..1117335 (-) 816 WP_207325657.1 pyrroline-5-carboxylate reductase -
  JYB88_RS04775 (JYB88_04775) - 1117478..1118185 (-) 708 WP_207325658.1 YggS family pyridoxal phosphate-dependent enzyme -
  JYB88_RS04780 (JYB88_04780) pilT 1118221..1119258 (+) 1038 WP_207322299.1 type IV pilus twitching motility protein PilT Machinery gene
  JYB88_RS04785 (JYB88_04785) pilU 1119281..1120393 (+) 1113 WP_207322300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JYB88_RS04790 (JYB88_04790) - 1120546..1121043 (+) 498 WP_410176168.1 glutathione peroxidase -
  JYB88_RS04795 (JYB88_04795) hemH 1121049..1122053 (+) 1005 WP_228289692.1 ferrochelatase -
  JYB88_RS04800 (JYB88_04800) ruvX 1122147..1122569 (-) 423 WP_207325660.1 Holliday junction resolvase RuvX -
  JYB88_RS04805 (JYB88_04805) - 1122577..1123140 (-) 564 WP_207325661.1 YqgE/AlgH family protein -
  JYB88_RS04810 (JYB88_04810) yciH 1123157..1123486 (-) 330 WP_207322304.1 stress response translation initiation inhibitor YciH -
  JYB88_RS04815 (JYB88_04815) - 1123648..1123989 (-) 342 WP_207325662.1 hypothetical protein -
  JYB88_RS04820 (JYB88_04820) - 1124209..1124766 (+) 558 WP_207326226.1 DUF4136 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41080.03 Da        Isoelectric Point: 6.1894

>NTDB_id=545761 JYB88_RS04785 WP_207322300.1 1119281..1120393(+) (pilU) [Shewanella cyperi strain FJAT-53726]
MDVRPFLKVMVERKASDLFITAGFPPSAKIDGELRPLAENAFNPAQALDFVESLMSDAQKKEFHETRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPEMDDLRLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAAMVGYRNGHARGHILTIED
PVEFVHDHRQSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKADGTGRRAAIEILINTPRVSSIIQKNELHLLKDTMAKSNEQGMQTFDQA
LFNLYQEGEISYADALHHADSPNDLRLMIKLASKDSGGSGFLEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=545761 JYB88_RS04785 WP_207322300.1 1119281..1120393(+) (pilU) [Shewanella cyperi strain FJAT-53726]
ATGGATGTACGTCCGTTTCTGAAGGTGATGGTCGAGCGCAAGGCCTCGGATCTGTTCATCACCGCAGGTTTCCCGCCCAG
CGCCAAGATAGACGGTGAGCTGCGGCCGCTGGCGGAAAATGCCTTCAACCCAGCTCAGGCGCTGGACTTTGTCGAGTCCT
TGATGTCTGACGCCCAGAAAAAAGAATTCCACGAGACCCGTGAATGTAACTTCGCCTTTGCGGCCAAGGAGCTGGGACGT
TTTCGTGTCAGCGCCTTCTGGCAGAGGGAATCTCCAGGTTGCGTGATGCGCCGTATCGAAACCAAGATCCCCGAGATGGA
CGATCTCAGGCTGCCGGCCATCCTCAAGGATCTGGTGATGAGCAAGCGCGGTCTTATCATAATGGTCGGTGGTACCGGCA
CGGGTAAGTCCACCTCGCTCGCCGCCATGGTGGGTTATCGCAACGGTCATGCCCGCGGTCATATTCTCACCATTGAAGAT
CCGGTGGAATTTGTTCACGACCACAGGCAAAGCATCATTACCCAGCGTGAGGTGGGCATAGACACCGACTCCTTCGACGC
GGCGCTGAAAAGCTCCCTGCGCCAGGCGCCGGATGTGATCCTTATCGGTGAGATCCGCAGCCAGGAAACCATGGAGTTCG
CCCTGTCGTTTGCCGAAACCGGTCACCTGTGTATGGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATGCACCTGGTGCCTGAAAGCAAACATCATCAATTGCTGTTTGACCTGTCGCTGAACCTGCGCGGCATTGTGGCTCAGCA
GCTGGTACCCAAGGCCGACGGCACCGGCCGCCGGGCAGCCATCGAAATTCTGATCAATACCCCCAGGGTGTCGAGCATCA
TCCAGAAGAACGAGCTGCACCTGCTGAAAGATACCATGGCCAAGTCCAATGAACAGGGAATGCAGACCTTCGACCAGGCG
CTGTTCAACCTGTATCAGGAAGGTGAAATCAGTTACGCCGATGCGCTGCACCATGCAGACTCGCCCAACGACCTGCGCCT
GATGATCAAGCTCGCCAGCAAGGATTCCGGCGGTTCGGGCTTTTTGGAAGGTGTGACCCTGGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Acinetobacter baylyi ADP1

60.845

95.946

0.584
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.436

97.838

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Acinetobacter baumannii strain A118

40.058

92.432

0.37
  pilT Acinetobacter nosocomialis M2

40.058

92.432

0.37
  pilT Acinetobacter baumannii D1279779

40.058

92.432

0.37
  pilT Legionella pneumophila strain Lp02

41.088

89.459

0.368
  pilT Legionella pneumophila strain ERS1305867

41.088

89.459

0.368
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362