Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   J0W32_RS06490 Genome accession   NZ_CP071317
Coordinates   1352781..1353218 (+) Length   145 a.a.
NCBI ID   WP_206862897.1    Uniprot ID   -
Organism   Acinetobacter indicus strain GXNN15X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1337337..1363907 1352781..1353218 within 0


Gene organization within MGE regions


Location: 1337337..1363907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0W32_RS14670 (J0W32_06420) - 1337337..1337972 (-) 636 WP_180088588.1 type IV pilin protein -
  J0W32_RS06425 (J0W32_06425) - 1337977..1341828 (-) 3852 WP_228289524.1 hypothetical protein -
  J0W32_RS06430 (J0W32_06430) - 1341838..1342593 (-) 756 WP_206862893.1 hypothetical protein -
  J0W32_RS06435 (J0W32_06435) - 1342604..1343602 (-) 999 WP_206862894.1 PilW family protein -
  J0W32_RS06440 (J0W32_06440) pilV 1343604..1344107 (-) 504 WP_171431621.1 type IV pilus modification protein PilV -
  J0W32_RS06445 (J0W32_06445) - 1344104..1344640 (-) 537 WP_206862895.1 Tfp pilus assembly protein FimT/FimU -
  J0W32_RS06450 (J0W32_06450) ispH 1344804..1345754 (-) 951 WP_045795274.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  J0W32_RS06455 (J0W32_06455) gmk 1345890..1346513 (+) 624 WP_045795273.1 guanylate kinase -
  J0W32_RS06460 (J0W32_06460) rpoZ 1346590..1346871 (+) 282 WP_004809957.1 DNA-directed RNA polymerase subunit omega -
  J0W32_RS06465 (J0W32_06465) - 1347094..1349193 (+) 2100 WP_160240914.1 RelA/SpoT family protein -
  J0W32_RS06470 (J0W32_06470) - 1349265..1349648 (+) 384 WP_005180461.1 RidA family protein -
  J0W32_RS06475 (J0W32_06475) - 1349803..1349997 (+) 195 WP_034597991.1 bacterioferritin-associated ferredoxin -
  J0W32_RS06480 (J0W32_06480) bfr 1350224..1350688 (+) 465 WP_005180468.1 bacterioferritin -
  J0W32_RS06485 (J0W32_06485) - 1350800..1352440 (-) 1641 WP_206862896.1 PglL family O-oligosaccharyltransferase -
  J0W32_RS06490 (J0W32_06490) pilE 1352781..1353218 (+) 438 WP_206862897.1 pilin Machinery gene
  J0W32_RS06495 (J0W32_06495) - 1353275..1355239 (+) 1965 WP_206862898.1 hypothetical protein -
  J0W32_RS06500 (J0W32_06500) - 1355229..1356080 (+) 852 WP_206862899.1 glycosyltransferase family 2 protein -
  J0W32_RS06505 (J0W32_06505) - 1356169..1357395 (+) 1227 WP_206862900.1 lipopolysaccharide biosynthesis protein -
  J0W32_RS06510 (J0W32_06510) - 1357376..1358497 (-) 1122 WP_206862901.1 glycosyltransferase family 4 protein -
  J0W32_RS06515 (J0W32_06515) - 1358502..1359614 (-) 1113 WP_206862902.1 glycosyltransferase -
  J0W32_RS06520 (J0W32_06520) - 1359611..1360513 (-) 903 WP_206862903.1 FkbM family methyltransferase -
  J0W32_RS06525 (J0W32_06525) - 1360515..1361297 (-) 783 WP_206862904.1 class I SAM-dependent methyltransferase -
  J0W32_RS06530 (J0W32_06530) - 1361657..1362718 (-) 1062 WP_206862905.1 UDP-N-acetylglucosamine 2-epimerase -
  J0W32_RS06535 (J0W32_06535) - 1362975..1363907 (+) 933 WP_206862906.1 IS5-like element IS17 family transposase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15134.17 Da        Isoelectric Point: 6.4460

>NTDB_id=544905 J0W32_RS06490 WP_206862897.1 1352781..1353218(+) (pilE) [Acinetobacter indicus strain GXNN15X4]
MNTMQKGFTLIELMIVVAIIGILAAIAIPAYQNYTKRSHVSEGLSLASGAKAAVTEFYSSNGHWPTGSNASVGLATPASI
NGNSVQSVTVAGSAIVVKYTDKVQNNAELALKGADVDGGITWECNTTDTNITLAVDPKYLPSNCR

Nucleotide


Download         Length: 438 bp        

>NTDB_id=544905 J0W32_RS06490 WP_206862897.1 1352781..1353218(+) (pilE) [Acinetobacter indicus strain GXNN15X4]
ATGAATACAATGCAGAAGGGCTTCACGCTCATCGAGCTTATGATTGTTGTCGCTATTATCGGTATTCTTGCTGCAATTGC
GATTCCTGCTTATCAAAACTACACGAAGCGTTCTCACGTTTCGGAAGGTTTAAGCTTAGCATCTGGTGCTAAAGCAGCGG
TAACTGAGTTTTATTCTTCTAATGGTCATTGGCCAACTGGCTCAAATGCTTCTGTAGGATTAGCTACTCCTGCAAGTATC
AATGGTAATTCAGTACAATCAGTTACAGTTGCTGGAAGCGCAATTGTAGTGAAATATACTGATAAAGTACAAAACAATGC
AGAGCTTGCATTAAAAGGTGCTGATGTAGATGGCGGTATTACTTGGGAATGTAACACTACTGATACTAATATTACGTTAG
CTGTAGATCCTAAATACTTACCATCTAATTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-145)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

46.541

100

0.51
  pilA Ralstonia pseudosolanacearum GMI1000

42.515

100

0.49
  pilE Neisseria gonorrhoeae MS11

44.375

100

0.49
  pilA2 Legionella pneumophila strain ERS1305867

47.183

97.931

0.462
  comP Acinetobacter baylyi ADP1

43.709

100

0.455
  pilA2 Legionella pneumophila str. Paris

46.479

97.931

0.455
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.096

100

0.414
  pilA Acinetobacter baumannii strain A118

39.597

100

0.407
  pilA Vibrio campbellii strain DS40M4

38.514

100

0.393
  pilA Haemophilus influenzae 86-028NP

42.105

91.724

0.386
  pilA Vibrio cholerae strain A1552

37.931

100

0.379
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.931

100

0.379
  pilA Vibrio cholerae C6706

37.931

100

0.379