Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   A0J47_RS03580 Genome accession   NZ_CP070624
Coordinates   730654..731034 (-) Length   126 a.a.
NCBI ID   WP_068945540.1    Uniprot ID   A0AAD3WVX4
Organism   Photobacterium damselae subsp. damselae strain RM-71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725654..736034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0J47_RS03560 (A0J47_003560) coaE 725999..726601 (-) 603 WP_068945536.1 dephospho-CoA kinase -
  A0J47_RS03565 (A0J47_003565) pilD 726603..727481 (-) 879 WP_068945537.1 prepilin peptidase Machinery gene
  A0J47_RS03570 (A0J47_003570) pilC 727664..728890 (-) 1227 WP_068945538.1 type II secretion system F family protein Machinery gene
  A0J47_RS03575 (A0J47_003575) pilB 728956..730647 (-) 1692 WP_068945539.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A0J47_RS03580 (A0J47_003580) pilA 730654..731034 (-) 381 WP_068945540.1 pilin Machinery gene
  A0J47_RS03585 (A0J47_003585) ampD 731420..731998 (+) 579 WP_068945541.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A0J47_RS03590 (A0J47_003590) pdhR 732358..733131 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  A0J47_RS03595 (A0J47_003595) aceE 733212..735872 (+) 2661 WP_068945542.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 12572.49 Da        Isoelectric Point: 9.0498

>NTDB_id=540938 A0J47_RS03580 WP_068945540.1 730654..731034(-) (pilA) [Photobacterium damselae subsp. damselae strain RM-71]
MKGQKGFTLIELMIVVAVIGVLSAIAIPQYQKYVQKGALASALATATALKTNYEDYVAVSGAAPTTGTDIGAVSFALGSI
TVTSGSIAANITKGGGKGNSVVLTRKDGAWSCTLSGAADITINGCS

Nucleotide


Download         Length: 381 bp        

>NTDB_id=540938 A0J47_RS03580 WP_068945540.1 730654..731034(-) (pilA) [Photobacterium damselae subsp. damselae strain RM-71]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGGTTATTGGCGTGTTATCAGCGATTGCGAT
TCCACAATATCAGAAGTATGTACAAAAAGGAGCTCTTGCTTCTGCACTAGCTACCGCTACTGCACTCAAAACTAACTATG
AAGATTATGTTGCCGTAAGTGGTGCAGCCCCCACAACAGGTACTGACATTGGAGCCGTATCATTCGCTCTTGGCAGCATA
ACTGTAACATCAGGTTCAATCGCCGCTAATATTACTAAAGGTGGTGGTAAAGGTAATAGTGTAGTTTTAACCCGAAAAGA
TGGAGCTTGGTCCTGTACTCTAAGCGGGGCTGCTGACATTACTATTAATGGCTGTAGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-114)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.972

100

0.492
  pilA Vibrio cholerae strain A1552

43.972

100

0.492
  pilA Vibrio cholerae C6706

43.972

100

0.492
  pilA Pseudomonas aeruginosa PAK

41.611

100

0.492
  pilA Acinetobacter baumannii strain A118

43.885

100

0.484
  pilA Vibrio parahaemolyticus RIMD 2210633

42.446

100

0.468
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.4

99.206

0.381
  pilA2 Legionella pneumophila str. Paris

40

95.238

0.381
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.167

95.238

0.373
  pilA2 Legionella pneumophila strain ERS1305867

39.167

95.238

0.373
  comP Acinetobacter baylyi ADP1

35.338

100

0.373
  comF Acinetobacter baylyi ADP1

33.824

100

0.365