Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A0J47_RS03575 Genome accession   NZ_CP070624
Coordinates   728956..730647 (-) Length   563 a.a.
NCBI ID   WP_068945539.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain RM-71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723956..735647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A0J47_RS03545 (A0J47_003545) mutT 724488..724895 (-) 408 WP_068945534.1 8-oxo-dGTP diphosphatase MutT -
  A0J47_RS03550 (A0J47_003550) yacG 724976..725185 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  A0J47_RS03555 (A0J47_003555) zapD 725194..725931 (-) 738 WP_068945535.1 cell division protein ZapD -
  A0J47_RS03560 (A0J47_003560) coaE 725999..726601 (-) 603 WP_068945536.1 dephospho-CoA kinase -
  A0J47_RS03565 (A0J47_003565) pilD 726603..727481 (-) 879 WP_068945537.1 prepilin peptidase Machinery gene
  A0J47_RS03570 (A0J47_003570) pilC 727664..728890 (-) 1227 WP_068945538.1 type II secretion system F family protein Machinery gene
  A0J47_RS03575 (A0J47_003575) pilB 728956..730647 (-) 1692 WP_068945539.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A0J47_RS03580 (A0J47_003580) pilA 730654..731034 (-) 381 WP_068945540.1 pilin Machinery gene
  A0J47_RS03585 (A0J47_003585) ampD 731420..731998 (+) 579 WP_068945541.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  A0J47_RS03590 (A0J47_003590) pdhR 732358..733131 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62510.09 Da        Isoelectric Point: 5.2594

>NTDB_id=540937 A0J47_RS03575 WP_068945539.1 728956..730647(-) (pilB) [Photobacterium damselae subsp. damselae strain RM-71]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDYEACCKAVYQRD
LVLRHRALPLAQNADTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=540937 A0J47_RS03575 WP_068945539.1 728956..730647(-) (pilB) [Photobacterium damselae subsp. damselae strain RM-71]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGCATTTTTAGCGATCGCGAGCTGGCCCAGCACCTTA
GCCATATTTTTGGTCTCACCCTCGTTTCAGTCCATGACTACGATTATGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGCGCACTGCCCCTCGCCCAAAATGCGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGCCGTAATATTGAACCGCTACTACTAGAAAACAAGCAGCTCGAATCAG
CTATCCGTCGTATTTATGGCCGTGAGTTAGGCGAAACCACCAATATGGCCAGTCGTGATGTCAGCGATGATGATCTGAAT
AACTTAGTCGATATTGGTGATGATGAGCTAGATCAAACCACAGATCTAGGCCAAGAAAACGAACCTGTTACTCGCTATAT
CAACCAAGTGCTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATTCATTTTGAACCTTATGAGACCAGTTATCGGATCC
GCTTTCGTTGTGATGGCATCTTGCACCAACACGCAACACCACCGGCCCACCTTTCTCGTCGTCTCTCAACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTGCTGCGTATTTTAGATAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAGCCGCAAGGGATG
ATCTTAATGACAGGGCCAACGGGCAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAAGATCCGGTAGAGATCAACTTACCCGGCATTAATCAGGTACACATTAACAATCAAGCAGGAC
TGGGATTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCCGATGTGGTGATGGTGGGGGAAATCCGCGATCTGGAA
ACCGCCTCTATTGCTGTTAAAGCTGCGCAAACTGGACACTTAGTACTATCGACTCTGCACACTAATTCTGCAGCCGAAAC
CATCACCCGCTTAACTAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGTAGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCGGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAAGT
GATGCCATTTAGTCGTCGGCTGGCTCAAGCTCTGGTTGATGGCGCGACTACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTGACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.167

100

0.622
  pilB Vibrio parahaemolyticus RIMD 2210633

61.279

100

0.613
  pilB Vibrio campbellii strain DS40M4

60.746

100

0.607
  pilB Legionella pneumophila strain ERS1305867

50.184

96.625

0.485
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50

94.494

0.472
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366