Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JJQ59_RS15735 Genome accession   NZ_CP068434
Coordinates   3403007..3404146 (-) Length   379 a.a.
NCBI ID   WP_013957931.1    Uniprot ID   G0EVU3
Organism   Cupriavidus necator strain C39.     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3398007..3409146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJQ59_RS15705 (JJQ59_15705) glcF 3398835..3400088 (+) 1254 WP_114133749.1 glycolate oxidase subunit GlcF -
  JJQ59_RS15710 (JJQ59_15710) - 3400168..3400545 (+) 378 WP_010815044.1 CidA/LrgA family protein -
  JJQ59_RS15715 (JJQ59_15715) - 3400542..3401267 (+) 726 WP_013957927.1 LrgB family protein -
  JJQ59_RS15720 (JJQ59_15720) - 3401353..3401724 (+) 372 WP_189374426.1 VOC family protein -
  JJQ59_RS15725 (JJQ59_15725) - 3401747..3402436 (+) 690 WP_114133751.1 helix-turn-helix transcriptional regulator -
  JJQ59_RS15730 (JJQ59_15730) - 3402440..3402934 (-) 495 WP_114133752.1 glutathione peroxidase -
  JJQ59_RS15735 (JJQ59_15735) pilU 3403007..3404146 (-) 1140 WP_013957931.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JJQ59_RS15740 (JJQ59_15740) pilT 3404173..3405216 (-) 1044 WP_013957933.1 type IV pilus twitching motility protein PilT Machinery gene
  JJQ59_RS15745 (JJQ59_15745) - 3405281..3405976 (+) 696 WP_114133753.1 YggS family pyridoxal phosphate-dependent enzyme -
  JJQ59_RS15750 (JJQ59_15750) proC 3406034..3406870 (+) 837 WP_114133754.1 pyrroline-5-carboxylate reductase -
  JJQ59_RS15755 (JJQ59_15755) ubiA 3406940..3407797 (-) 858 WP_114133755.1 4-hydroxybenzoate octaprenyltransferase -
  JJQ59_RS15760 (JJQ59_15760) - 3407939..3408424 (-) 486 WP_010815054.1 Dps family protein -
  JJQ59_RS15765 (JJQ59_15765) - 3408517..3408714 (-) 198 WP_013957937.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42097.35 Da        Isoelectric Point: 6.6384

>NTDB_id=529049 JJQ59_RS15735 WP_013957931.1 3403007..3404146(-) (pilU) [Cupriavidus necator strain C39.]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKIKPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPATLHEVVMAKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMVSFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSASAKDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=529049 JJQ59_RS15735 WP_013957931.1 3403007..3404146(-) (pilU) [Cupriavidus necator strain C39.]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCTGACCTGTT
CATCACTTCTGACTTCCCGCCTGCGATCAAGGTCGACGGCAAGATCAAGCCGGTGTCGCAGCAGCCGCTGAACCCGACCC
AGGCACTGGGCCTGGTGCGCTCGGTGATGAACGAGCGCCAGGTGCAGGACTTCGACACCAGCCGCGAATGCAATTTCGCG
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTTTCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGCACCAT
CAATACGCGCATCCCCTCGGTGGCCGACCTGGACCTGCCGGCGACGCTGCATGAGGTGGTGATGGCCAAGCGCGGGCTGG
TGATCGTGACCGGCGCCACCGGCTCGGGCAAGTCGACCACGCTGGCGGCAATGCTGGATCACCGCAACGCGCATTCGTAC
GGCCATATCATCACCATCGAGGATCCGATCGAGTACGTGCATGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGAGTCCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GTGACCGCGAGACCATGGAGTACGCGATGCAGTACGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGATCTCGCAGCGCCTGTTGCCGCGCGCCGGCCGCAAGGGGCGGGTGCCGGCGGTGGAGATCATGATCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAAGGCAAGATCACCTACGAGGACGCGCTGCGCAACGC
CGACTCGCTCAACGACCTGCGCCTGATGATCAAGCTGCACAGCGCGAGCGCCAAGGACACCGACCTTGGCGCCGGTACCG
AGCATCTCAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0EVU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.571

92.348

0.578
  pilU Acinetobacter baylyi ADP1

56.825

94.723

0.538
  pilU Vibrio cholerae strain A1552

53.444

95.778

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

43.323

88.918

0.385
  pilT Acinetobacter baumannii D1279779

42.943

87.863

0.377
  pilT Acinetobacter nosocomialis M2

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.943

87.863

0.377
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Legionella pneumophila strain Lp02

42.342

87.863

0.372
  pilT Legionella pneumophila strain ERS1305867

42.342

87.863

0.372
  pilT Neisseria meningitidis 8013

40.294

89.71

0.361