Detailed information
Bioinformatically predicted
Overview
| Name | pilA/pilAI | Type | Machinery gene |
| Locus tag | JH296_RS05615 | Genome accession | NZ_CP066986 |
| Coordinates | 1327500..1327907 (+) | Length | 135 a.a. |
| NCBI ID | WP_129588891.1 | Uniprot ID | - |
| Organism | Xanthomonas campestris pv. campestris strain 15-1 | ||
| Function | assembly of type IV pilus (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Genomic island | 1308097..1326730 | 1327500..1327907 | flank | 770 |
Gene organization within MGE regions
Location: 1308097..1327907
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JH296_RS05530 (JH296_05520) | - | 1308097..1308447 (+) | 351 | WP_274316879.1 | hypothetical protein | - |
| JH296_RS05535 (JH296_05525) | - | 1308737..1308967 (-) | 231 | WP_165349436.1 | hypothetical protein | - |
| JH296_RS05540 (JH296_05530) | - | 1309031..1309186 (+) | 156 | WP_129588884.1 | hypothetical protein | - |
| JH296_RS05545 (JH296_05535) | - | 1309363..1309758 (+) | 396 | WP_016945003.1 | hypothetical protein | - |
| JH296_RS05550 (JH296_05540) | - | 1309849..1310241 (+) | 393 | WP_129588885.1 | H-NS family nucleoid-associated regulatory protein | - |
| JH296_RS05555 (JH296_05545) | glgX | 1310776..1312905 (-) | 2130 | WP_129588886.1 | glycogen debranching protein GlgX | - |
| JH296_RS05560 (JH296_05550) | rimK | 1313398..1314273 (+) | 876 | WP_011038222.1 | 30S ribosomal protein S6--L-glutamate ligase | - |
| JH296_RS05565 (JH296_05555) | - | 1314923..1315600 (+) | 678 | WP_003490678.1 | response regulator transcription factor | - |
| JH296_RS05570 (JH296_05560) | - | 1315593..1316927 (+) | 1335 | WP_162808569.1 | HAMP domain-containing sensor histidine kinase | - |
| JH296_RS05575 (JH296_05565) | - | 1317070..1317561 (-) | 492 | WP_011038220.1 | GNAT family N-acetyltransferase | - |
| JH296_RS05580 (JH296_05570) | - | 1317558..1317848 (-) | 291 | WP_014508712.1 | DUF1778 domain-containing protein | - |
| JH296_RS05585 (JH296_05575) | - | 1317924..1318351 (-) | 428 | Protein_1076 | SymE family type I addiction module toxin | - |
| JH296_RS05590 (JH296_05580) | - | 1318990..1323750 (-) | 4761 | WP_274497101.1 | RHS repeat-associated core domain-containing protein | - |
| JH296_RS05595 (JH296_05585) | coaE | 1323895..1324515 (-) | 621 | WP_129588888.1 | dephospho-CoA kinase | - |
| JH296_RS05600 (JH296_05590) | - | 1324529..1325392 (-) | 864 | WP_129588889.1 | A24 family peptidase | - |
| JH296_RS05605 (JH296_05595) | pilC | 1325399..1326655 (-) | 1257 | WP_129589289.1 | type II secretion system F family protein | Machinery gene |
| JH296_RS05610 (JH296_05600) | pilA/pilAI | 1327015..1327425 (+) | 411 | WP_274497102.1 | pilin | Machinery gene |
| JH296_RS05615 (JH296_05605) | pilA/pilAI | 1327500..1327907 (+) | 408 | WP_129588891.1 | pilin | Machinery gene |
Sequence
Protein
Download Length: 135 a.a. Molecular weight: 14013.19 Da Isoelectric Point: 8.1079
>NTDB_id=523905 JH296_RS05615 WP_129588891.1 1327500..1327907(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 15-1]
MSGQKGFSLIELMIVIAIIAILAAIAFPAYQSSVAKAQLTAALAEIRPGKTTLETVVQDGRDPSLVDKSYVGLTVSARCS
SVSAILDSSGVGEISCTLQGSSLVSGRDLKLKRSAAGLWTCDASAFDQKYRPAGC
MSGQKGFSLIELMIVIAIIAILAAIAFPAYQSSVAKAQLTAALAEIRPGKTTLETVVQDGRDPSLVDKSYVGLTVSARCS
SVSAILDSSGVGEISCTLQGSSLVSGRDLKLKRSAAGLWTCDASAFDQKYRPAGC
Nucleotide
Download Length: 408 bp
>NTDB_id=523905 JH296_RS05615 WP_129588891.1 1327500..1327907(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 15-1]
ATGTCTGGGCAAAAAGGCTTTTCTCTAATAGAACTGATGATTGTTATAGCGATTATTGCCATATTGGCTGCTATAGCATT
CCCCGCTTATCAAAGTTCAGTTGCAAAAGCGCAGCTTACCGCTGCATTAGCCGAGATAAGGCCAGGGAAAACCACGCTCG
AAACAGTGGTCCAGGATGGTAGGGATCCGTCACTCGTTGATAAAAGTTACGTGGGTTTGACTGTATCCGCGAGGTGTTCC
AGCGTCAGTGCGATTTTGGATTCCTCAGGGGTTGGTGAGATTAGTTGTACCCTCCAAGGATCATCCCTGGTTTCAGGGCG
TGATTTAAAGTTGAAGCGTTCTGCCGCTGGTCTCTGGACCTGTGATGCTTCTGCTTTTGACCAAAAATATCGCCCTGCTG
GTTGTTGA
ATGTCTGGGCAAAAAGGCTTTTCTCTAATAGAACTGATGATTGTTATAGCGATTATTGCCATATTGGCTGCTATAGCATT
CCCCGCTTATCAAAGTTCAGTTGCAAAAGCGCAGCTTACCGCTGCATTAGCCGAGATAAGGCCAGGGAAAACCACGCTCG
AAACAGTGGTCCAGGATGGTAGGGATCCGTCACTCGTTGATAAAAGTTACGTGGGTTTGACTGTATCCGCGAGGTGTTCC
AGCGTCAGTGCGATTTTGGATTCCTCAGGGGTTGGTGAGATTAGTTGTACCCTCCAAGGATCATCCCTGGTTTCAGGGCG
TGATTTAAAGTTGAAGCGTTCTGCCGCTGGTCTCTGGACCTGTGATGCTTCTGCTTTTGACCAAAAATATCGCCCTGCTG
GTTGTTGA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| pilA/pilAI | Pseudomonas stutzeri DSM 10701 |
47.794 |
100 |
0.481 |
| pilA | Acinetobacter baumannii strain A118 |
43.885 |
100 |
0.452 |
| pilA | Pseudomonas aeruginosa PAK |
39.597 |
100 |
0.437 |
| pilA/pilAII | Pseudomonas stutzeri DSM 10701 |
41.481 |
100 |
0.415 |
| pilA | Ralstonia pseudosolanacearum GMI1000 |
33.951 |
100 |
0.407 |
| comP | Acinetobacter baylyi ADP1 |
36.735 |
100 |
0.4 |
| pilA | Vibrio cholerae O1 biovar El Tor strain E7946 |
37.063 |
100 |
0.393 |
| pilA | Vibrio cholerae strain A1552 |
37.063 |
100 |
0.393 |
| pilA | Vibrio cholerae C6706 |
37.063 |
100 |
0.393 |
| pilE | Neisseria gonorrhoeae MS11 |
32.121 |
100 |
0.393 |
| pilA2 | Legionella pneumophila str. Paris |
37.5 |
100 |
0.378 |
| pilA2 | Legionella pneumophila strain ERS1305867 |
36.765 |
100 |
0.37 |