Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   JH296_RS05610 Genome accession   NZ_CP066986
Coordinates   1327015..1327425 (+) Length   136 a.a.
NCBI ID   WP_274497102.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 15-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1308097..1326730 1327015..1327425 flank 285


Gene organization within MGE regions


Location: 1308097..1327425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH296_RS05530 (JH296_05520) - 1308097..1308447 (+) 351 WP_274316879.1 hypothetical protein -
  JH296_RS05535 (JH296_05525) - 1308737..1308967 (-) 231 WP_165349436.1 hypothetical protein -
  JH296_RS05540 (JH296_05530) - 1309031..1309186 (+) 156 WP_129588884.1 hypothetical protein -
  JH296_RS05545 (JH296_05535) - 1309363..1309758 (+) 396 WP_016945003.1 hypothetical protein -
  JH296_RS05550 (JH296_05540) - 1309849..1310241 (+) 393 WP_129588885.1 H-NS family nucleoid-associated regulatory protein -
  JH296_RS05555 (JH296_05545) glgX 1310776..1312905 (-) 2130 WP_129588886.1 glycogen debranching protein GlgX -
  JH296_RS05560 (JH296_05550) rimK 1313398..1314273 (+) 876 WP_011038222.1 30S ribosomal protein S6--L-glutamate ligase -
  JH296_RS05565 (JH296_05555) - 1314923..1315600 (+) 678 WP_003490678.1 response regulator transcription factor -
  JH296_RS05570 (JH296_05560) - 1315593..1316927 (+) 1335 WP_162808569.1 HAMP domain-containing sensor histidine kinase -
  JH296_RS05575 (JH296_05565) - 1317070..1317561 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH296_RS05580 (JH296_05570) - 1317558..1317848 (-) 291 WP_014508712.1 DUF1778 domain-containing protein -
  JH296_RS05585 (JH296_05575) - 1317924..1318351 (-) 428 Protein_1076 SymE family type I addiction module toxin -
  JH296_RS05590 (JH296_05580) - 1318990..1323750 (-) 4761 WP_274497101.1 RHS repeat-associated core domain-containing protein -
  JH296_RS05595 (JH296_05585) coaE 1323895..1324515 (-) 621 WP_129588888.1 dephospho-CoA kinase -
  JH296_RS05600 (JH296_05590) - 1324529..1325392 (-) 864 WP_129588889.1 A24 family peptidase -
  JH296_RS05605 (JH296_05595) pilC 1325399..1326655 (-) 1257 WP_129589289.1 type II secretion system F family protein Machinery gene
  JH296_RS05610 (JH296_05600) pilA/pilAI 1327015..1327425 (+) 411 WP_274497102.1 pilin Machinery gene

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14020.37 Da        Isoelectric Point: 8.4937

>NTDB_id=523904 JH296_RS05610 WP_274497102.1 1327015..1327425(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 15-1]
MKKQNGFTLIELMIVIAIIAILAAIALPMYQDYVAKSQVTAGLAEINPGKTQYEVALNEGKTTVTGIGDLGLKSPTERCT
ISPITALSATGTIVCTLKGNTQVVGKTVTLTRATDGTWTCATNAAAKHAPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=523904 JH296_RS05610 WP_274497102.1 1327015..1327425(+) (pilA/pilAI) [Xanthomonas campestris pv. campestris strain 15-1]
ATGAAGAAGCAGAACGGTTTTACGCTGATCGAACTGATGATTGTTATTGCGATCATTGCAATCCTGGCCGCCATCGCGCT
CCCGATGTACCAGGACTACGTCGCCAAGTCGCAGGTGACTGCGGGCCTGGCTGAAATCAATCCTGGCAAGACGCAGTACG
AAGTTGCACTGAACGAAGGCAAGACCACGGTCACCGGCATCGGCGATCTCGGCCTGAAGTCGCCGACCGAGCGCTGCACC
ATCAGCCCCATCACTGCACTGAGCGCCACCGGCACCATCGTCTGCACGCTCAAGGGCAATACCCAGGTTGTCGGTAAGAC
CGTCACCCTGACCCGTGCTACTGATGGCACGTGGACCTGCGCGACCAATGCTGCTGCCAAGCATGCGCCAGCAGGCTGCC
CGGGCGTCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

53.333

99.265

0.529
  pilA Acinetobacter baumannii strain A118

51.825

100

0.522
  pilA Pseudomonas aeruginosa PAK

41.892

100

0.456
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.776

98.529

0.441
  pilA2 Legionella pneumophila str. Paris

41.429

100

0.426
  pilA Vibrio cholerae C6706

40.426

100

0.419
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.426

100

0.419
  pilA Vibrio cholerae strain A1552

40.426

100

0.419
  pilA2 Legionella pneumophila strain ERS1305867

40.714

100

0.419
  comP Acinetobacter baylyi ADP1

37.584

100

0.412