Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JH286_RS05370 Genome accession   NZ_CP066939
Coordinates   1217472..1217900 (+) Length   142 a.a.
NCBI ID   WP_057671660.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain 12112     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1212472..1222900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH286_RS05340 (JH286_05345) - 1212575..1213066 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH286_RS05345 (JH286_05350) - 1213063..1213353 (-) 291 WP_011038219.1 DUF1778 domain-containing protein -
  JH286_RS05350 (JH286_05355) - 1213428..1213829 (-) 402 WP_040940795.1 SymE family type I addiction module toxin -
  JH286_RS05355 (JH286_05360) coaE 1214361..1214984 (-) 624 WP_011038217.1 dephospho-CoA kinase -
  JH286_RS05360 (JH286_05365) - 1214998..1215861 (-) 864 WP_057671659.1 A24 family peptidase -
  JH286_RS05365 (JH286_05370) pilC 1215868..1217130 (-) 1263 WP_057671824.1 type II secretion system F family protein Machinery gene
  JH286_RS05370 (JH286_05375) pilA 1217472..1217900 (+) 429 WP_057671660.1 pilin Machinery gene
  JH286_RS05375 (JH286_05380) pilB 1218036..1219766 (+) 1731 WP_057671826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH286_RS05380 (JH286_05385) - 1219954..1221321 (-) 1368 WP_011035783.1 IS5-like element IS1478 family transposase -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14272.35 Da        Isoelectric Point: 9.1206

>NTDB_id=523428 JH286_RS05370 WP_057671660.1 1217472..1217900(+) (pilA) [Xanthomonas campestris pv. campestris strain 12112]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTVRGRVSEAMVAASAAKTVVAENAANGSALDSGWTAPTATNNVASVA
VATATGNITVTTTAKAGGGTIIFAPSANGAALASGTVPTDRISWDCKGGTLAAKYRPAECRT

Nucleotide


Download         Length: 429 bp        

>NTDB_id=523428 JH286_RS05370 WP_057671660.1 1217472..1217900(+) (pilA) [Xanthomonas campestris pv. campestris strain 12112]
ATGAAAAAGCAACAAGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGCAATCCTTGCCGCCATCGCGCT
GCCGGCTTATCAGGACTACACCGTTCGCGGTCGTGTCTCTGAAGCAATGGTTGCTGCCTCTGCTGCCAAGACGGTTGTGG
CCGAGAATGCTGCGAACGGCTCCGCCCTAGACAGTGGTTGGACCGCTCCTACGGCAACTAACAATGTCGCCAGCGTCGCT
GTTGCTACCGCTACGGGCAATATCACTGTTACTACCACTGCCAAGGCCGGTGGCGGCACGATAATCTTTGCTCCCTCGGC
TAACGGTGCTGCACTGGCTTCCGGTACTGTTCCAACCGATCGTATTTCTTGGGATTGCAAGGGCGGCACCTTGGCTGCCA
AGTACCGTCCAGCTGAATGCCGCACCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

52.201

100

0.585
  pilA2 Legionella pneumophila str. Paris

54.795

100

0.563
  pilA2 Legionella pneumophila strain ERS1305867

54.795

100

0.563
  comP Acinetobacter baylyi ADP1

47.771

100

0.528
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

39.247

100

0.514
  pilA/pilA1 Eikenella corrodens VA1

40

100

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.541

100

0.423
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.136

100

0.415
  pilA Acinetobacter baumannii strain A118

40.426

99.296

0.401
  pilA Vibrio cholerae C6706

33.766

100

0.366
  pilA Vibrio cholerae strain A1552

33.766

100

0.366
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

33.766

100

0.366