Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   K5A80_RS23665 Genome accession   NZ_CP081477
Coordinates   4939375..4939848 (-) Length   157 a.a.
NCBI ID   WP_217950248.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain P8W     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4934375..4944848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5A80_RS23645 (K5A80_24405) ampD 4935116..4935682 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5A80_RS23650 (K5A80_24410) - 4935833..4938088 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  K5A80_RS23655 (K5A80_24415) nadC 4938374..4939222 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5A80_RS23665 (K5A80_24425) pilE 4939375..4939848 (-) 474 WP_217950248.1 pilin Machinery gene
  K5A80_RS23670 (K5A80_24430) pilB 4940079..4941779 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5A80_RS23675 (K5A80_24435) pilC 4941783..4943000 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  K5A80_RS23680 (K5A80_24440) pilD 4943003..4943875 (+) 873 WP_023911898.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K5A80_RS23685 (K5A80_24445) coaE 4943872..4944483 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  K5A80_RS23690 (K5A80_24450) yacG 4944480..4944680 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 15991.31 Da        Isoelectric Point: 8.0955

>NTDB_id=522610 K5A80_RS23665 WP_217950248.1 4939375..4939848(-) (pilE) [Pseudomonas aeruginosa strain P8W]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLGGKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=522610 K5A80_RS23665 WP_217950248.1 4939375..4939848(-) (pilE) [Pseudomonas aeruginosa strain P8W]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGGGGGGGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

47.059

97.452

0.459
  pilE Neisseria gonorrhoeae MS11

42.515

100

0.452
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.176

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

42.593

100

0.439
  pilA2 Legionella pneumophila str. Paris

46.259

93.631

0.433
  pilA2 Legionella pneumophila strain ERS1305867

44.898

93.631

0.42
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.42
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.333

95.541

0.414
  pilA Pseudomonas aeruginosa PAK

41.722

96.178

0.401
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

96.815

0.363