Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K3T18_RS25545 Genome accession   NZ_CP081287
Coordinates   5437620..5438072 (-) Length   150 a.a.
NCBI ID   WP_028681203.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F092021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5432620..5443072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3T18_RS25530 (K3T18_25530) - 5433774..5434922 (-) 1149 WP_172773772.1 DUF6531 domain-containing protein -
  K3T18_RS25535 (K3T18_25535) - 5437101..5437343 (+) 243 WP_023109353.1 hypothetical protein -
  K3T18_RS25545 (K3T18_25545) pilA 5437620..5438072 (-) 453 WP_028681203.1 pilin Machinery gene
  K3T18_RS25550 (K3T18_25550) pilB 5438304..5440004 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K3T18_RS25555 (K3T18_25555) pilC 5440008..5441228 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  K3T18_RS25560 (K3T18_25560) pilD 5441232..5442104 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  K3T18_RS25565 (K3T18_25565) coaE 5442101..5442712 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  K3T18_RS25570 (K3T18_25570) yacG 5442709..5442909 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15671.99 Da        Isoelectric Point: 7.7813

>NTDB_id=522005 K3T18_RS25545 WP_028681203.1 5437620..5438072(-) (pilA) [Pseudomonas aeruginosa strain F092021]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVDIKDTGDGTVKFTFATGQSSPKNAGTAITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=522005 K3T18_RS25545 WP_028681203.1 5437620..5438072(-) (pilA) [Pseudomonas aeruginosa strain F092021]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACTACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAGATATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCACGGCAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.789

100

0.667
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.958

95.333

0.4
  pilA2 Legionella pneumophila strain ERS1305867

38.926

99.333

0.387
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA Acinetobacter nosocomialis M2

42.537

89.333

0.38
  pilA Ralstonia pseudosolanacearum GMI1000

39.583

96

0.38